公开(公告)号：EP0528877A1

公开(公告)日：1993-03-03

申请号：EP91909082.0

申请日：1991-05-06

申请人： BIOMETIC APS, c/o Dansk Udviklingsfinansiering A/S

发明人： SPINELL, Max

IPC分类号： G01N15 ,  G01N33 ,  G01N1 ,  G01N21

CPC分类号： G01N15/10 ,  G01N1/38 ,  G01N15/1404 ,  G01N21/64 ,  G01N2015/1037 ,  G01N2015/1254 ,  G01N2015/1409 ,  G01N2015/1418 ,  Y10S436/805 ,  Y10S436/806

摘要： L'appareil comprend une cuvette comportant un premier élément ainsi qu'un second élément dotés chacun d'une surface plane. Les premier et second éléments sont mobiles l'un par rapport à l'autre entre une première position dans laquelle les surfaces sont en contact étanche au fluide l'une avec l'autre, et une seconde position dans laquelle les surfaces sont espacées. Dans la première position, une zone d'écoulement allongée est définie par une gorge située dans une des surfaces. Une admission permettant l'alimentation en liquide de la zone d'écoulement et une sortie permettant la décharge du liquide de ladite zone d'écoulement sont ménagées dans un des éléments. Les éléments sont transparents à la lumière à laquelle les particules sont exposées, et ils sont transparents au rayonnement émis par les particules. On a également prévu deux électrodes permettant de procéder à un choix de particules en suspension dans le liquide.

公开(公告)号：EP2397836B1

公开(公告)日：2018-01-10

申请号：EP11003575.5

申请日：2011-05-02

申请人： Sony Corporation

发明人： Shinoda, Masataka ,  Matsui, Takeshi ,  Tsuji, Akiko ,  Yamasaki, Takeshi ,  Akiyama, Shoji

IPC分类号： G01N15/14

CPC分类号： B01L3/502761 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/502776 ,  B01L9/527 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/049 ,  G01N15/1404 ,  G01N15/1484 ,  G01N2015/1418 ,  G01N2015/149

摘要： Disclosed herein is a microchip including a substrate and a sample flow path within the substrate. The sample flow path includes a changing flow path and a microtube connected to the changing flow path. The changing flow path is configured to change a cross sectional shape of the sample flow path from a quadrangular shape at a first end to a circular shape at a second end. The microtube is connected to the second end of the changing flow path and is disposed within the substrate.

公开(公告)号：EP3087194A1

公开(公告)日：2016-11-02

申请号：EP14874631.6

申请日：2014-12-11

申请人： Becton, Dickinson and Company

发明人： WARNER, Brian David ,  YU, Liping ,  GHANEKAR, Smita ,  CAO, Jianying

IPC分类号： C12Q1/02 ,  G01N15/14 ,  G01N21/64

CPC分类号： G01N1/286 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0487 ,  G01N2001/4094 ,  G01N2015/1006 ,  G01N2015/1418

摘要： Aspects of the present disclosure include methods for processing a biological sample. Methods according to certain embodiments include disrupting a biological sample to produce a disrupted biological sample and acoustically separating larger components from smaller components in the disrupted biological sample. In certain embodiments, methods may include monitoring aggregation while the biological sample is being processed. Methods, in certain instances, also include acoustically separating cells from cellular debris and non-cellular macromolecules as well as magnetically separating magnetically labelled moieties from unlabeled moieties. Systems, including a disrupter, one or more acoustic concentrator devices, feedback monitors and magnetic separation devices suitable for practicing the subject methods are also described.

公开(公告)号：EP0528877B1

公开(公告)日：1995-03-08

申请号：EP91909082.9

申请日：1991-05-06

申请人： BIOMETIC APS, c/o Dansk Udviklingsfinansiering A/S

发明人： SPINELL, Max

IPC分类号： G01N15/14 ,  G01N21/05

CPC分类号： G01N15/10 ,  G01N1/38 ,  G01N15/1404 ,  G01N21/64 ,  G01N2015/1037 ,  G01N2015/1254 ,  G01N2015/1409 ,  G01N2015/1418 ,  Y10S436/805 ,  Y10S436/806

摘要： The apparatus comprises a cuvette comprising a first body member and a second body member each having a planar facial surface. The first and the second body members are movable relative to one another between a first position, in which the surfaces are in fluid-tight contact with one another, and a second position, in which the surfaces are spaced apart. In the first position, an elongated flow area is defined by a groove provided in one of the surfaces. An inlet for feeding the liquid to the flow area and an outlet for discharging the liquid from the flow area are provided in one of the body members. The body members are transparent to light to which the particles are exposed and further transparent to the radiation emitted by the particles. Two electrodes are further provided for carrying out an election of particles suspended in the liquid.

公开(公告)号：EP3087194A4

公开(公告)日：2017-11-01

申请号：EP14874631

申请日：2014-12-11

申请人： BECTON DICKINSON CO

发明人： WARNER BRIAN DAVID ,  YU LIPING ,  GHANEKAR SMITA ,  CAO JIANYING

IPC分类号： G01N1/28 ,  B01L3/00 ,  C12Q1/02 ,  G01N1/40 ,  G01N15/10 ,  G01N21/64

CPC分类号： G01N1/286 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0487 ,  G01N2001/4094 ,  G01N2015/1006 ,  G01N2015/1418

摘要： Aspects of the present disclosure include methods for processing a biological sample. Methods according to certain embodiments include disrupting a biological sample to produce a disrupted biological sample and acoustically separating larger components from smaller components in the disrupted biological sample. In certain embodiments, methods may include monitoring aggregation while the biological sample is being processed. Methods, in certain instances, also include acoustically separating cells from cellular debris and non-cellular macromolecules as well as magnetically separating magnetically labelled moieties from unlabeled moieties. Systems, including a disrupter, one or more acoustic concentrator devices, feedback monitors and magnetic separation devices suitable for practicing the subject methods are also described.

公开(公告)号：EP1062494A4

公开(公告)日：2010-10-06

申请号：EP99934338

申请日：1999-02-19

申请人： XY LLC ,  CYTOMATION INC

发明人： BUCHANAN KRISTOPHER S ,  HERICKHOFF LISA ,  MALACHOWSKI GEORGE C ,  OTTENBERG MATTHIAS J G ,  SEIDEL GEORGE ,  FERGUSON DOUGLAS H

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N2015/1418 ,  G01N2015/149

公开(公告)号：EP1062494B1

公开(公告)日：2015-06-24

申请号：EP99934338.7

申请日：1999-02-19

申请人： XY, LLC ,  Cytomation, Inc.

发明人： BUCHANAN, Kristopher, S. ,  HERICKHOFF, Lisa ,  MALACHOWSKI, George, C. ,  OTTENBERG, Matthias, J., G. ,  SEIDEL, George ,  FERGUSON, Douglas, H.

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N2015/1418 ,  G01N2015/149

公开(公告)号：EP2397836A1

公开(公告)日：2011-12-21

申请号：EP11003575.5

申请日：2011-05-02

申请人： Sony Corporation

发明人： Shinoda, Masataka ,  Matsui, Takeshi ,  Tsuji, Akiko ,  Yamasaki, Takeshi ,  Akiyama, Shoji

IPC分类号： G01N15/14

CPC分类号： B01L3/502761 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/502776 ,  B01L9/527 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/049 ,  G01N15/1404 ,  G01N15/1484 ,  G01N2015/1418 ,  G01N2015/149

摘要： Disclosed herein is a microchip including a substrate and a sample flow path within the substrate. The sample flow path includes a changing flow path and a microtube connected to the changing flow path. The changing flow path is configured to change a cross sectional shape of the sample flow path from a quadrangular shape at a first end to a circular shape at a second end. The microtube is connected to the second end of the changing flow path and is disposed within the substrate.

摘要翻译： 本文公开了一种微芯片，其包括衬底和衬底内的样品流动路径。 样品流动路径包括变化的流动路径和连接到变化的​​流动路径的微管。 变化流路构造成将样品流路的截面形状从第一端的四边形变为第二端的圆形。 微管连接到变化流路的第二端并设置在衬底内。

公开(公告)号：EP1623206A1

公开(公告)日：2006-02-08

申请号：EP04731920.7

申请日：2004-05-10

申请人： Evotec Technologies GmbH

发明人： MÜLLER, Torsten ,  HUMMEL, Stefan ,  PFENNIG, Annette

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N2015/1409 ,  G01N2015/1413 ,  G01N2015/1418

摘要： The invention relates to a particle injector (15) for introducing particles into a carrier flow of a microfluidic system, especially for injecting biological cells into the carrier flow of a cell sorter. The particle injector comprises an inlet for receiving the carrier flow, an outlet for discharging the carrier flow comprising the introduced particles, a carrier flow channel which connects the inlet to the outlet, and an injection channel flowing into the carrier flow channel for introducing the particles into the carrier flow. The inventive particle injector is characterized in that the carrier flow channel has substantially no dead volume.

公开(公告)号：EP1062494A1

公开(公告)日：2000-12-27

申请号：EP99934338.7

申请日：1999-02-19

申请人： XY, Inc. ,  Cytomation, Inc. ,  C.S.U.R.F.

发明人： BUCHANAN, Kristopher, S. ,  HERICKHOFF, Lisa ,  MALACHOWSKI, George, C. ,  OTTENBERG, Matthias, J., G. ,  SEIDEL, George ,  FERGUSON, Douglas, H.

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N2015/1418 ,  G01N2015/149

摘要： A flow cytometry system is disclosed for oscillating the exterior of a flow cytometer nozzle. Oscillating the exterior of a flow cytometer nozzle at selected oscillations or a sweeping range of oscillations disintegrates particles and gas entrapped within the flow cytometer nozzle. Adherents absorbed to the exterior of the flow cytometer nozzler may also be removed. A energy source (1) serves to generate a selected oscillation or range of oscillations. The energy source (1) is connected to a flexible transfer element (2) which conducts the electrical signals to an oscillation system (3). The oscillation system (3) turns the electrical energy from the energy source (1) into mechanical oscillations and is connected to a portable device (4) which disintegrates particles, dislocates gas within the flow cytometer nozzle and cleans the exterior of the flow cytometer aperture.