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公开(公告)号：EP1070141A1
公开(公告)日：2001-01-24
申请号：EP99905756.5
申请日：1999-02-05
发明人： WORLEY, Paul, F. , LANAHAN, Anthony , GOETZ, Bernard , HIEMISCH, Holger , KUNER, Rohini , SCHEEK, Sigrid , NIKOLICH, Karoly , ZHUKOVSKI, Eugene
CPC分类号： C12Q1/6883 , A61K38/00 , C07K14/47 , C12Q1/6876 , C12Q2600/136 , C12Q2600/158
摘要： The present invention provides methods and materials related to immediate early genes. Specifically, the invention provides isolated immediate early gene nucleic acid, cells that contain isolated immediate early gene nucleic acid, substantially pure polypeptides encoded by immediate early gene nucleic acid, and antibodies having specific binding affinity for a polypeptide encoded by immediate early gene nucleic acid. In addition, the invention provides cDNA libraries enriched for immediate early genes cDNAs, isolated nucleic acid derived from such cDNA libraries, and methods for treating conditions related to a deficiency in a neuron's immediate early gene responsiveness to a stimulus.
摘要翻译： 本发明提供了与即时早期基因有关的方法和材料。 具体而言，本发明提供了分离的立即早期基因核酸，含有分离的立即早期基因核酸的细胞，由立即早期基因核酸编码的基本上纯的多肽和对由立即早期基因核酸编码的多肽具有特异性结合亲和力的抗体。 另外，本发明提供了富含立即早期基因cDNA的cDNA文库，源自这种cDNA文库的分离的核酸，以及用于治疗与神经元对刺激的即时早期基因响应缺陷有关的病症的方法。
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公开(公告)号：EP1062366A1
公开(公告)日：2000-12-27
申请号：EP99911289.9
申请日：1999-03-10
发明人： SIDRANSKY, David
CPC分类号： C07K14/4703 , A61B10/0051 , C07K14/82 , C12Q1/6827 , C12Q1/6886 , C12Q2600/156
摘要： Methods for detection of a cell proliferative disorder, such as cancer, are provided utilizing analysis of target mutant nucleic acids in saliva specimens are described. The presence of target mutant nucleic acids is indicative of a neoplastic disorder of the lung or the head and neck.
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公开(公告)号：EP1056881A1
公开(公告)日：2000-12-06
申请号：EP99908441.1
申请日：1999-02-25
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2830/38
摘要： Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. This invention describes a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production can be conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system expedites the process of generating and testing recombinant adenoviruses.
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公开(公告)号：EP1011657A1
公开(公告)日：2000-06-28
申请号：EP98934479.1
申请日：1998-07-10
发明人： SANDERS, Scherer, P. , PROUD, David
IPC分类号： A61K31/195
CPC分类号： A61K31/15 , A61K9/0043 , A61K9/0073 , A61K31/00 , A61K31/198
摘要： Nitric oxide generating compounds or compounds which induce in situ synthesis of nitric oxide can be used to inhibit rhinovirus infection. Nitric oxide has the ability to inhibit both viral replication as well as the synthesis of cytokines, in particular the proinflammatory cytokines. Thus the symptoms of rhinovirus infections can be ameliorated by treatments to increase nitric oxide in the respiratory tract.
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公开(公告)号：EP0989849A2
公开(公告)日：2000-04-05
申请号：EP98928941.8
申请日：1998-06-11
发明人： WALSH, Scott , RUBENSTEIN, Ronald , ZEITLIN, Pamela , LEONG, Kam
CPC分类号： A61K9/5169 , A61K31/192 , A61K45/06 , A61K47/62 , A61K47/6435 , A61K47/6929 , A61K48/00 , B82Y5/00 , Y10S977/884 , Y10S977/906 , Y10S977/915 , Y10S977/92 , Y10S977/923
摘要： 4-Phenylbutyrate exerts many beneficial biological effects. It appears to induce the transcription of certain promoters, as well as having a remedial effect on proteins which are aberrantly localized within the cell. In addition, it appears to cause cells to developmentally differentiate. The present invention provides nanosphere formulations of 4-phenylbutyrate and other drugs which remediate defective protein localization intracellularly. These formulations permit lower concentrations of drugs to be administered, providing both cost and safety benefits.
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公开(公告)号：EP0956365A1
公开(公告)日：1999-11-17
申请号：EP97942383.0
申请日：1997-08-28
发明人： SIDRANSKY, David
IPC分类号： C12Q1
CPC分类号： C12Q1/6883 , C12Q1/6886 , C12Q2600/112 , C12Q2600/118 , C12Q2600/156 , C12Q2600/158 , C12Q2600/172
摘要： The present invention relates to the detection of a cell proliferative disorder associated with alterations of microsatellite DNA in a sample. The microsatellite DNA can be contained within any of a variety of samples, such as urine, sputum, bile, stool, cervical tissue, saliva, tears or cerebral spinal fluid. The invention is a method to detect an allelic imbalance by assaying microsatellite DNA. Allelic imbalance is detected by observing an abnormality in an allele, such as an increase or decrease in microsatellite DNA which is at or corresponds to an allele. An increase can be detected as the appearance of a new allele. In practicing the invention, DNA amplification methods, particularly polymerase chain reactions, are useful for amplifying the DNA. DNA analysis methods can be used to detect such a decrease or increase. The invention is also a method to detect genetic instability of microsatellite DNA. Genetic instability is detected by observing an amplification or deletion of the small, tandem repeat DNA sequences in the microsatellite DNA which is at or corresponds to an allele. The invention is also a kit for practicing these methods.
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公开(公告)号：EP0954608A1
公开(公告)日：1999-11-10
申请号：EP97927933.0
申请日：1997-06-03
CPC分类号： C12Q1/6827 , C12Q1/6886 , C12Q2600/154 , C12Q2600/156 , C12Q2523/125 , C12Q2531/113 , C12Q2525/185 , C12Q2535/125 , C12Q2521/331
摘要： The present invention provides a method of PCR, methylation specific PCR (MSP), for rapid identification of DNA methylation patterns in a CpG-containing nucleic acid. MSP uses the PCR reaction itself to distinguish between methylated and unmethylated DNA, which adds an improved sensitivity of methylation detection.
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公开(公告)号：EP0948651A1
公开(公告)日：1999-10-13
申请号：EP97954272.0
申请日：1997-12-23
发明人： CHATTERJEE, Subroto
CPC分类号： C12N9/16 , A61K38/00 , C07K2319/00
摘要： Isolated nucleic acids are provided that encode human neutral sphingomyelinase (N-SMase) and N-SMase fragments and derivatives capable of hybridizing to such N-SMase-encoding nucleic acids. The invention also includes isolated recombinant human neutral sphingomyelinase (N-SMase) and N-SMase fragments and derivatives are also provided. Novel assays are also provided to identify compounds useful in the diagnosis or treatment of human neutral sphingomyelinase related disorders.
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公开(公告)号：EP0927046A1
公开(公告)日：1999-07-07
申请号：EP96937646.0
申请日：1996-10-01
发明人： DIETZ, Harry, C.
IPC分类号： C12N15 , A01K67 , A61K31 , A61K48 , A61P3 , A61P35 , A61P43 , C07K14 , C12N1 , C12N5 , C12N9 , C12P21 , C12Q1 , G01N33 , A61K38
CPC分类号： G01N33/5008 , A61K38/00 , A61K48/00 , C07K2319/00 , C12N9/14 , G01N33/5091 , G01N33/68
摘要： All eukaryotes that have been studied to date possess the ability to detect and degrade transcripts that contain a premature signal for the determination of translation. This process of nonsense-mediated RNA decay (NMRD) has been most comprehensively studied in the yeast Saccharomyces cerevisiae where at least three trans-acting factors (Upf1p through Upf3p) are required. The present invention provides cDNAs encoding human and murine RENT1(regulator of nonsense transcripts). RENT1 is the first identified mammalian protein that contains all of the putative functional elements in Upf1p including zinc finger-like motifs and NTPase domains as well as all motifs common to members of helicase superfamily I. Moreover, expression of a chimeric protein, containing the central region of RENT1 flanked by the extreme N- and C-termini of Upf1p, complements the Upf1p-deficient phenotype in yeast.
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公开(公告)号：EP0904292A1
公开(公告)日：1999-03-31
申请号：EP97917735.0
申请日：1997-03-31
发明人： LEE, Se-Jin , RANKIN, Christopher
IPC分类号： C12N15 , A61K31 , A61K38 , A61K39 , A61K48 , A61K49 , A61P37 , C07K14 , C07K16 , C12N1 , C12N5 , C12P21 , C12Q1 , G01N33
CPC分类号： C07K14/475 , A61K38/00 , C07K14/495 , C07K14/52
摘要： Growth differentiation factor-14 (GDF-14) is disclosed along with its polynucleotide sequence and amino acid sequence. Also disclosed are diagnostic and therapeutic methods of using the GDF-14 polypeptide and polynucleotide sequences.
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