摘要:
In a method for preparing clonogenic fibroblasts, tissue is taken from the donor and the cells isolated from the tissue, the cell suspension so obtained is sieved, the cells contained in the cell suspension are grown and the cells are transferred to a tissue culture; alternatively, the cells are isolated by mechanical comminution followed by enzymatic treatment with collagenase alone. As a result of the transfection into the fibroblasts, at least one gene which codes for a biologically active protein, preferably a therapeutically active protein such as a growth factor, a hormone, an enzyme, a clotting factor or a clotting inhibitor, is inwardly transferred.
摘要:
A surgical instrument (1) is used to clamp a vessel (6), hollow organ or the like. The instrument has essentially a plumb (3) with a thread holder (4) and a shaft-like plumb holder (5) that can be coupled with the plumb. The plumb holder can be coupled with the plumb (3) and has actuating members (7) for opening and closing the plumb thread holder. One thread end (12) is connected to the plumb (3) and can be grasped with forceps (25) that are extended through the instrument and can be drawn through using two mutally spring-actuated jaws (9). A loop is formed around the vessel (6) and the thread drawn to clamp off this vessel. After plumb (3) and plumb holder (5) have been detached from each other, the drawn-through thread end is clamped and the plumb holder (5) as well as the forceps (25) can then be removed. The thread (2) can be varied in tension as required and can be untied again by opening the clamping device, preferably using the plumb holder (5).