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公开(公告)号：EP0069450A1
公开(公告)日：1983-01-12
申请号：EP82302523.4
申请日：1982-05-18
摘要： Vitamin B 12 in liquid samples, such as human serum, is assayed by a competitive binding technique using intrinsic factor and certain labelled vitamin B 12 derivatives. The labelled derivatives are formed from the (d)-monocarboxylic acid isomer of vitamin B 12 , which isomer is free from other monocarboxylic acid isomers (and derivatives thereof) of vitamin B 12 , by binding to the (d)-isomer, via the carboxylic group, a compound which is itself a label (e.g. an enzyme) or which comprises a label (e.g. a fluorophore), or to which a label is attached (e.g. a histidine ester to which 125| is attached). The labelled derivatives of the (d)-monocarboxylic acid of vitamin B 12 , free from other isomeric monocarboxylic acids and derivatives, are novel and constitute one aspect of the invention.
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公开(公告)号：EP0051985A3
公开(公告)日：1982-08-11
申请号：EP81305261
申请日：1981-11-05
CPC分类号： G01N33/563 , Y10S436/825
摘要： In the immunoassay of a particular protein in a biological fluid, there is frequently interference in the assay by other proteins present in the fluid, e.g. by complement factors or antibodies in human serum.The interference so caused can be avoided by subjecting the fluid to protein-digestion, using for example an enzyme such as pepsin, as a result of which the particular protein of interest, or a fragment thereof, can be assayed without interference by the other proteins. Also, radioallergosorbent tests for particular IgE antibodies can be improved in sensitivity and accuracy, by subjecting the absorbed IgE to enzymic digestion, and then assaying a fragment thereof.
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公开(公告)号：EP0010932B1
公开(公告)日：1982-02-17
申请号：EP79302342.5
申请日：1979-10-26
IPC分类号： G01N33/54
CPC分类号： G01N33/53 , G01N33/564 , Y10S435/962 , Y10S435/968 , Y10S436/821
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84.发明公开Electrochemical sensor for analysis of analytes in liquids 失效在Flüssigkeiten分析von Bestandteilen的ElektrochemischerFühlerzur。
公开(公告)号：EP0012035A1
公开(公告)日：1980-06-11
申请号：EP79302760.8
申请日：1979-12-03
发明人： Pace, Salvatore J.
CPC分类号： G01N33/492 , Y10S435/817
摘要： Sensors for the analysis of analytes in liquids are known. Conventionally many such sensors require an external reference cell. The invention provides an electrochemical sensor with a built-in reference. In particular, a sensor of the invention includes a pair of electrodes (95a, 95b) the first electrode being electrically connected to a permeable layer (91a) containing a given concentration of the species to be measured, and the second electrode being electrically connected to a permeable layer (916) which is either free from species, or contains an amount thereof different from that in the first layer, the second layer being accessible (in use of the sensor) to the liquid sample under assay. The invention also provides an array of sensors for simultaneous analysis of a multiplicity of analytes in a liquid sample, at least one sensor in the array being as defined above.
摘要翻译： 用于分析液体中分析物的传感器是已知的。 通常许多这样的传感器需要外部参考单元。 本发明提供具有内置参考的电化学传感器。 特别地，本发明的传感器包括一对电极（95a，95b），第一电极电连接到含有给定浓度的被测物种的可渗透层（91a），第二电极电连接到 不含物质的渗透层（91b）或含有与第一层不同的量的可渗透层（91b），第二层在测定时可接触（使用传感器）至液体样品。 本发明还提供了用于同时分析液体样品中多种分析物的传感器阵列，阵列中的至少一个传感器如上所述。
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公开(公告)号：EP0010932A1
公开(公告)日：1980-05-14
申请号：EP79302342.5
申请日：1979-10-26
IPC分类号： G01N33/54
CPC分类号： G01N33/53 , G01N33/564 , Y10S435/962 , Y10S435/968 , Y10S436/821
摘要： A liquid, particularly but not exclusively a liquid of human origin such as blood serum or urine, is immunoas- sayed for antibodies, antigens or antibody: antigen complexes, using as a reagent in the analysis an active fraction from mouse ascitic fluid. This active fraction is a euglobulin and has the ability, like human C1q, to combine with antibody: antigen complexes but not with free antibody or antigen. Unlike human Clq, however, it remains active at high pH's and its activity is not destroyed by 0.1M putrescine or 0.1M hydrazine, so analyses on human body fluids can be carried out at high pH's or in the presence of putrescine and hydrazine, without interference from endogenous human Clq. The active fraction is a very broadly applicable reagent in immunoassays and particularly useful in techniques involving agglutination of latex particles.
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