公开(公告)号：EP0051985A3

公开(公告)日：1982-08-11

申请号：EP81305261

申请日：1981-11-05

申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)

发明人： Magnusson, Carl-Gustav M. ,  Masson, Pierre L. ,  Collet-Cassart, Daniel

IPC分类号： G01N33/54 ,  C12Q01/34

CPC分类号： G01N33/563 ,  Y10S436/825

摘要： In the immunoassay of a particular protein in a biological fluid, there is frequently interference in the assay by other proteins present in the fluid, e.g. by complement factors or antibodies in human serum.The interference so caused can be avoided by subjecting the fluid to protein-digestion, using for example an enzyme such as pepsin, as a result of which the particular protein of interest, or a fragment thereof, can be assayed without interference by the other proteins. Also, radioallergosorbent tests for particular IgE antibodies can be improved in sensitivity and accuracy, by subjecting the absorbed IgE to enzymic digestion, and then assaying a fragment thereof.