公开(公告)号：EP4350355A3

公开(公告)日：2024-07-10

申请号：EP24158438.2

申请日：2017-02-24

申请人： The Binding Site Group Limited

发明人： HARDING, Stephen ,  ASHBY, Jamie

IPC分类号： G01N33/563

CPC分类号： G01N33/563

摘要： 1. An assay kit for use in a method of quantifying a multimeric free light chain in a sample the method comprising:
(i) treating the multimeric free light chain with a separating agent selected to convert at least a portion of the multimeric free light chain into monomeric free light chain;
(ii) binding the free light chain to a free light chain-specific binding agent;
(iii) comparing the amount of binding of the free light chain to the free light chain-specific binding agent to a calibration curve, wherein the calibration curve is obtained (i) by binding the free light chain-specific binding agent to one or more predetermined amounts of separating agent treated free light chain, prior to contacting with free light chain-specific binding agent or (ii) by binding the free light chain-specific binding agent to a predetermined amount of substantially monomeric free light chain; and
(iv) determining an amount of the multimeric analyte in the sample, the kit comprising a free light chain specific binding agent and a separating agent for converting at least a portion of a free light chain analyte into monomeric free light chain.

公开(公告)号：EP3362785A1

公开(公告)日：2018-08-22

申请号：EP16854672.9

申请日：2016-10-14

申请人： University Health Network (UHN) ,  The Governing Council of the University of Toronto

发明人： KESHAVJEE, Shafique ,  LIU, Mingyao ,  SAGE, Andrew ,  SARGENT, Edward ,  KELLEY, Shana

IPC分类号： G01N27/416 ,  G01N27/327 ,  G01N33/543 ,  G01N27/30

CPC分类号： G01N27/3276 ,  B82Y30/00 ,  G01N27/3278 ,  G01N33/5438 ,  G01N33/563 ,  G01N33/566 ,  G01N2800/56 ,  G01N2800/60

摘要： There is described herein a method for the electrochemical quantification of a protein analyte in sample, comprising: providing one or more electrode(s), each comprising at least one peptide attached to its surface, the peptide being the protein or a fragment thereof; contacting the sample and electrode with an antibody in the presence of a redox reporter, wherein the antibody is capable of binding to each of the protein analyte and the peptide on the electrode; measuring an electrochemical signal generated by the redox reporter when a potential is applied; quantifying the protein analyte by comparing the electrochemical signal generated with a control, wherein the electrochemical signal is indirectly proportional to the amount of protein analyte in the sample.

公开(公告)号：EP3114481A1

公开(公告)日：2017-01-11

申请号：EP15710227.8

申请日：2015-03-04

申请人： Mologic Limited

发明人： DAVIS, Paul ,  HEMMINGTON, Sandra

IPC分类号： G01N33/53

CPC分类号： G01N33/689 ,  G01N33/536 ,  G01N33/543 ,  G01N33/563 ,  G01N33/58 ,  G01N2333/59

摘要： Methods are provided for determining the presence of an analyte present in a liquid sample involving: contacting the liquid sample with a predetermined amount of an analogue of the analyte and an excess of a first binding moiety, wherein the first binding moiety is capable of binding each of the analyte and the analogue independently; contacting the mixture with a second binding moiety; and determining the level of a signal indicative of the presence of the analogue-first binding moiety complex bound to the second binding moiety, wherein if the level of the signal determined is lower than the level of a maximum signal determined when no analyte is present, then analyte is present in the sample. Corresponding kits are also provided.

摘要翻译： 提供了用于确定存在于液体样品中的分析物的存在的方法，包括：使液体样品与预定量的分析物类似物和过量的第一结合部分接触，其中第一结合部分能够结合 分析物和类似物的独立性; 使混合物与第二结合部分接触; 以及确定指示与第二结合部分结合的类似物优先结合部分复合物的存在的信号的水平，其中如果确定的信号的水平低于不存在分析物时确定的最大信号的水平， 那么分析物存在于样品中。 还提供了相应的套件。

公开(公告)号：EP2369345B1

公开(公告)日：2014-10-01

申请号：EP10011475.0

申请日：2004-07-02

申请人： Institut Pasteur ,  INSERM - Institut National de la Santé et de la Recherche Médicale ,  Centre National de la Recherche Scientifique (CNRS)

发明人： Phalipon, Armelle ,  Nato, Farida ,  Mulard, Laurence ,  Sansonetti, Philippe ,  Baleux, Francoise ,  Belot, Frédéric ,  Grandjean, Cyrille

IPC分类号： G01N33/569 ,  C07K16/12 ,  A61K47/48 ,  C07H3/06 ,  G01N33/563 ,  A61K39/112

CPC分类号： A61K39/0283 ,  A61K47/557 ,  A61K47/61 ,  A61K47/6415 ,  A61K47/646 ,  A61K2039/6031 ,  A61K2039/6037 ,  A61K2039/625 ,  C07H3/06 ,  C07K16/1228 ,  C07K2317/565 ,  C08B37/006 ,  G01N33/563 ,  G01N33/56916 ,  Y02A50/476

摘要： The present invention relates to a saccharide selected from the group consisting of: €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ{B(E)CD} €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ{(E)CDAB}n €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ{AB(E)CD}n Wherein A, B, C, E, D and n are as defined in claim 1. The invention also relates to derivatives thereof, pharmaceutical compositions comprising said derivatives, and kit for the diagnostic of Shigella flexneri type 2a infection comprising said derivatives.

公开(公告)号：EP1746162A2

公开(公告)日：2007-01-24

申请号：EP05077801.8

申请日：1994-01-31

申请人： Bristol-Myers Squibb Company

发明人： Ledbetter, Jeffrey A. ,  Gilliland, Lisa K. ,  Hayden, Martha S. ,  Linsley, Peter S. ,  Bajorath, Jurgen, Rhein. Friedrich-Wilhelms Univ. ,  Fell, Perry H.

IPC分类号： C12N15/79 ,  C12N15/62 ,  C07K16/46 ,  C12N5/10 ,  C07K16/28 ,  C07K16/30

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7155 ,  C07K16/00 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/2827 ,  C07K16/30 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/74 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/30 ,  C07K2319/43 ,  C12N15/62 ,  C12N2740/16122 ,  G01N33/563 ,  G01N33/56972 ,  G01N33/57492 ,  G01N2333/705

摘要： The present invention provides an expression vector encoding monospecific or bispecific fusion protein.
In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen.
In another embodiment the expression vector encodes a. bispecific fusion protein, which vector comprises a recombinant bispecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen and a DNA sequence encoding a second binding domain capable of binding a cell surface antigen, each domain capable of binding a different antigen.
The present invention also provides a method for producing a biologically active monospecific or bispecific fusion protein in a mammalian cell. This method comprises: (a) transfecting the mammalian cell with the recombinant expression vector of the invention; (b) culturing the mammalian cell so transfected in step (a); and (c) recovering the biologically active bispecific fusion protein so as produced by the cultured mammalian cell.

摘要翻译： 本发明提供了对表达矢量编码双特异性融合蛋白。 在一个实施例中的表达载体编码一个单特异性融合蛋白，载体，其包含重组单特异性单链盒，其包含编码能够结合细胞表面抗原的第一结合结构域的DNA序列。 该表达载体编码的双特异性融合蛋白，其载体包含重组双特异性单链盒，其包含编码能够结合细胞表面抗原和DNA序列编码能够结合细胞表面抗原的第二结合域的第一结合结构域的DNA序列 ，能够结合不同抗原的每个域。 本发明因此提供了用于在哺乳动物细胞中生产的生物活性的双特异性融合蛋白的方法。 此方法包括：（a）用本发明的重组表达载体的哺乳动物细胞; （B）培养在步骤如此转染的哺乳动物细胞（a）的 和（c）回收所述的生物活性的双特异性融合蛋白，以便通过培养的哺乳动物细胞中产生。

公开(公告)号：EP1592445A4

公开(公告)日：2007-01-10

申请号：EP01989275

申请日：2001-10-24

申请人： AGNELLO VINCENT

发明人： AGNELLO VINCENT

IPC分类号： A61K45/00 ,  A61K38/00 ,  A61K38/17 ,  A61K38/46 ,  A61K39/395 ,  A61P31/12 ,  C07K14/705 ,  C07K16/28 ,  C12Q1/44 ,  G01N33/563 ,  G01N33/569 ,  G01N33/92 ,  A61K39/42 ,  C07K1/00 ,  C12N5/06 ,  C12Q1/70

CPC分类号： G01N33/92 ,  A61K38/177 ,  A61K38/465 ,  A61K2039/505 ,  C07K16/28 ,  C07K2317/54 ,  C07K2317/76 ,  C07K2317/77 ,  C12Q1/44 ,  G01N33/563 ,  G01N33/56983

公开(公告)号：EP0610046B1

公开(公告)日：2005-12-21

申请号：EP94300692.4

申请日：1994-01-31

申请人： Bristol-Myers Squibb Company

发明人： Ledbetter, Jeffrey A. ,  Gilliland, Lisa K. ,  Hayden, Martha S. ,  Linsley, Peter S. ,  Bajorath, Jurgen ,  Fell, Perry H.

IPC分类号： C12N15/62 ,  C12N5/10 ,  C12P21/00 ,  C07K19/00 ,  A61K39/395 ,  G01N33/563

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K2039/505 ,  C07K14/70521 ,  C07K14/7155 ,  C07K16/00 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/2827 ,  C07K16/30 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/74 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/30 ,  C07K2319/43 ,  C12N15/62 ,  C12N2740/16122 ,  G01N33/563 ,  G01N33/56972 ,  G01N33/57492 ,  G01N2333/705

公开(公告)号：EP1088227A1

公开(公告)日：2001-04-04

申请号：EP99928306.2

申请日：1999-05-14

申请人： Amersham Pharmacia Biotech AB

发明人： STEINDL, Franz

IPC分类号： G01N33/53

CPC分类号： G01N33/68 ,  G01N33/563 ,  Y10S435/814 ,  Y10S435/961 ,  Y10S436/825 ,  Y10S436/826 ,  Y10T436/25 ,  Y10T436/25125

摘要： The present invention relates to a method for the quantitative release of natural or recombinant proteins, polypeptides or peptides (thermostable immunoligands) able to bind to the Fc-part of immunoglobulins (antibodies, in particular of the IgG class and primarily becoming bound outside the paratope) from complexes in various sample matrixes in order to make these released natural or recombinant proteins, polypeptides or peptides quantitatively available in immunochemical assays and to keep them quantitatively available. The method is characterized by mixing the sample with reagent compound that is able to bind non-specifically to immunoglobulins, and thereafter subjecting the sample to a heat treatment step followed by a cooling step.

公开(公告)号：EP0630475B1

公开(公告)日：2000-09-06

申请号：EP93906293.1

申请日：1993-03-05

申请人： QUIDEL CORPORATION

发明人： BURD, John ,  MILLER, Steven ,  ROWLEY, Gerald ,  PRONOVOST, Allan

IPC分类号： G01N31/22 ,  G01N33/53 ,  G01N33/543 ,  G01N33/563

CPC分类号： G01N33/558 ,  G01N33/54366 ,  G01N33/556 ,  G01N33/563 ,  G01N33/80 ,  Y10S435/962 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/825

摘要： A method for preparing a blood fluid sample substantially free from red blood cells (RBCs) is disclosed. The method involves applying whole blood to a solid support matrix which contains reagent such as antibodies or their immunologically reactive fragments that are directed against RBCs or their components. The invention further comprises a method and device for the testing of analytes in blood. Whole blood is applied to the device, which then separates the RBCs from the plasma. The device further contains zones and and reagents for analysis of the plasma.

公开(公告)号：EP0444561B1

公开(公告)日：1996-11-20

申请号：EP91102712.6

申请日：1991-02-25

申请人： BOEHRINGER MANNHEIM GMBH

发明人： Deger, Arno, Dr. ,  Schenk, Roland, Dr. ,  Bienhaus, Gerhard, Dr.

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/563 ,  G01N33/58 ,  C12Q1/68

CPC分类号： G01N33/58 ,  G01N33/53 ,  G01N33/54306 ,  G01N33/563 ,  G01N33/78