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    Use of R-genes as a selection marker in plant transformation and use of cisgenes in plant transformation
    81.
    发明公开
    Use of R-genes as a selection marker in plant transformation and use of cisgenes in plant transformation 审中-公开
    的R基因用作植物转化的选择标记并使用同源转基因的植物转化

    公开(公告)号：EP1949785A1

    公开(公告)日：2008-07-30

    申请号：EP07101275.1

    申请日：2007-01-26

    申请人： Coöperatie AVEBE U.A.

    发明人： The designation of the inventor has not yet been filed

    IPC分类号： A01H5/00 ,  C12N15/82

    CPC分类号： C12N15/821 ,  C12N15/8282

    摘要： The invention relates to the field of plant transformation, in particular plant transformation of a Solanaceae, preferably of potato. The present invention provides an alternative selection method in plant transformation processes. The invention further provides a plant that has been provided with additional nucleic acid sequences but which genetically modified plant essentially consists of cis plant sequences, for example a genetically modified potato plant that has been provided with additional (essentially) potato plant sequences. Such a transgenic plant is herein further referred to as a cis-gene plant.

    摘要翻译： 本发明涉及植物转化领域，计划优选马铃薯的在茄科的特定变换。 本发明提供了计划在转化过程的替代选择方法。 本发明提供了进一步的计划并已经设置有额外的核酸序列，但其中遗传修饰的本质besteht顺植物序列，例如遗传修饰的马铃薯植物并已经设置有额外的（基本上）马铃薯植物序列计划。 这样的转基因植物在此外，通过对被称为顺式发电设备。

    Promotion of somatic embryogenesis in plants by Wuschel gene expression
    82.
    发明公开
    Promotion of somatic embryogenesis in plants by Wuschel gene expression 有权
    Förderungder Somatische胚胎发育在Pflanzen durch die表达des Wuschel Gens

    公开(公告)号：EP1785481A1

    公开(公告)日：2007-05-16

    申请号：EP07075071.6

    申请日：2002-10-28

    申请人： THE ROCKEFELLER UNIVERSITY

    发明人： Zuo, Jianru ,  Niu, Qi-Wen ,  Frugis, Giovanna ,  Chua, Nam-Hai

    IPC分类号： C12N5/04 ,  C12N5/10 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  A01H4/00

    CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

    摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

    摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无剪切力。 在另一个实施方案中，这种过表达可用于产生可用于产生双倍体植物的单倍体植物。

    DNA CONSTRUCTS AND METHODS TO ENHANCE THE PRODUCTION OF COMMERCIALLY VIABLE TRANSGENIC PLANTS
    84.
    发明公开
    DNA CONSTRUCTS AND METHODS TO ENHANCE THE PRODUCTION OF COMMERCIALLY VIABLE TRANSGENIC PLANTS 有权
    DNA结构和方法提高生产商业变现转基因植物

    公开(公告)号：EP1613755A2

    公开(公告)日：2006-01-11

    申请号：EP04749936.3

    申请日：2004-04-09

    申请人： Monsanto Technology LLC

    发明人： GILBERTSON, Larry ,  KRIEGER, Elysia ,  ZHANG, Wanggen ,  YE, Xudong

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/821 ,  C12N15/8212

    摘要： The present invention has incorporated a non-lethal negative selectable marker gene into the vector backbone DNA of a DNA plasmid used to transform plant cells. These transgenes are designed to express a non-lethal gene product in plant cells that contain the vector backbone DNA of the DNA plasmid. The gene products of the non-lethal negative selectable marker gene are involved in plant hormone biosynthesis pathways, plant hormone substrate diversion, plant hormone degradation, plant hormone signaling or metabolic interference. The use of these DNA plasmids to transform plant cells provides for the enhanced production of commercially viable plants.

    COMPOSITIONS AND METHODS FOR THE MODULATION OF GENE EXPRESSION IN PLANTS
    88.
    发明公开
    COMPOSITIONS AND METHODS FOR THE MODULATION OF GENE EXPRESSION IN PLANTS 无效
    组合物和方法进行基因表达在植物调制

    公开(公告)号：EP1551967A2

    公开(公告)日：2005-07-13

    申请号：EP03765827.5

    申请日：2003-07-21

    申请人： UNIVERSITY OF SOUTH CAROLINA

    发明人： VANCE BOWMAN, Vicki ,  BOWMAN, Lewis, Howard ,  MALLORY, Allison

    IPC分类号： C12N15/09 ,  C12N15/11 ,  C12N15/63 ,  C12N15/82 ,  C12N15/86 ,  C12N15/87 ,  C12N15/90 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/821 ,  C12N15/8216 ,  C12N15/8218

    摘要： Compositions and methods for modulating nucleotide sequence expression, particularly for modulating gene expression in plants, are provided. The compositions comprise precursor RNA constructs for the expression of an RNA precursor. The precursor RNA construct comprises a promoter that is expressed in a plant cell driving the expression of a precursor RNA having a micrORNA. The mRNA is complementary or partially complementary to a portion of a target gene or nucleotide sequence and function to modulate expression of the target sequence or gene. In this manner, the RNA precursor construct can be designed to modulate expression of any nucleotide sequence of interest, either an endogenous plant gene or alternatively a transgene. The precursor RNA constructs may be used in combination with modulators to enhance the effect on gene expression. Expression of a modulator in the presence of the precursor RNA alters the accumulation of miRNAs and thus enhances the regulatory capabilities of miRNAs. The invention further comprises the use of a modulator to control gene expression via both the siRNA and the miRNA pathway. Transformed plants, tissues, cells and seeds are also provided.