公开(公告)号：EP4437855A1

公开(公告)日：2024-10-02

申请号：EP22898622.0

申请日：2022-11-24

申请人： Amano Enzyme Inc.

发明人： TAKAYAMA Hiroyuki

IPC分类号： A23L5/00 ,  A23C11/10 ,  A23L2/38 ,  A23L11/60 ,  A23L11/65 ,  C12N9/04

CPC分类号： A23C11/10 ,  A23L2/38 ,  A23L5/00 ,  A23L11/60 ,  A23L11/65 ,  C12N9/0004

摘要： To provide a technique for reducing saccharides in plant-base drinks or foods.
The present technique provides a method for producing plant-base drinks or foods and a method for reducing saccharides in plant-base drinks or foods, which include a step for causing a maltotriose-generating enzyme to act on a plant-base raw material. In the production method according to the present technique, a step for causing a carbohydrate oxidase to act on a plant-base raw material can be further performed. The present technique also provides an enzymatic agent that is for reducing saccharides and that contains a maltotriose-generating enzyme. The enzymatic agent for reducing saccharides according to the present technique can further contain a carbohydrate oxidase.

公开(公告)号：EP2961402B1

公开(公告)日：2024-10-02

申请号：EP14757297.8

申请日：2014-02-27

IPC分类号： A61K31/352 ,  A61K9/127 ,  C12P17/06 ,  C12N9/02 ,  C12N9/10

CPC分类号： A61K31/352 ,  C12Y121/03007 ,  C12Y121/03008 ,  C12Y203/01206 ,  C12N9/0004 ,  C12N9/1029 ,  C12Y205/01102 ,  Y02A50/30

公开(公告)号：EP4368732A3

公开(公告)日：2024-08-28

申请号：EP24165738.6

申请日：2020-07-08

申请人： Asymchem Life Science (Tianjin) Co., Ltd.

发明人： HONG, Hao ,  JAMES, Gage ,  XIAO, Yi ,  ZHANG, Na ,  LIU, Fang ,  WANG, Zujian ,  SHI, Wanming ,  YAN, Junjie ,  ZHANG, Mujiao

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N15/70 ,  C12N1/21 ,  C12P7/02 ,  C12R1/19

CPC分类号： C12N9/0004 ,  C12N15/70 ,  C12P7/02 ,  C12Y101/01 ,  C12R2001/1920210501

摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.

公开(公告)号：EP4361274A1

公开(公告)日：2024-05-01

申请号：EP22828505.2

申请日：2022-06-23

申请人： Amano Enzyme Inc.

发明人： YACHI, Hiroyuki ,  SAKAI, Kiyota

IPC分类号： C12N15/53 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/63

CPC分类号： C12N9/0004 ,  C12N15/80 ,  C12N15/81 ,  C12N15/63 ,  C12N15/74 ,  C12N5/10

摘要： Provided is a laccase that exhibits excellent activity in pH ranges including the alkaline range. According to the present invention, a laccase having an amino acid sequence of a laccase derived from Paramyrothecium sp., and a laccase having a sequence that is similar thereto and that includes said amino acid sequence as the basic backbone exhibit excellent activity in pH ranges including the alkaline range.

公开(公告)号：EP4361260A1

公开(公告)日：2024-05-01

申请号：EP22827500.4

申请日：2022-06-20

申请人： Porton Pharma Solutions Ltd.

发明人： CHEN, Zecong

IPC分类号： C12N9/06 ,  C12N15/53 ,  C12N11/089 ,  C12N1/21 ,  C12P17/16

CPC分类号： C12P17/16 ,  C12N15/66 ,  C12N9/0004 ,  C12R2001/1920210501 ,  C12N15/70 ,  C12P41/00 ,  C12N15/74 ,  C12N11/089

摘要： Disclosed in the present invention are an imine reductase mutant, and a method for preparing (S)-nicotine using the imine reductase mutant. The first objective of the invention is to provide an imine reductase mutant, for solving the problems in the prior art that no nicotine of S configuration can be obtained by using an enzyme and the enzyme activity is low, and this mutant can implement catalytic reduction of a substrate myosmine or 4-(Methylamino)-1-(pyridin-3-yl)butanone to (S)-nicotine. The second objective of the present invention is to provide a coexpressed enzyme of the imine reductase and a glucose dehydrogenase, specifically being a coexpressed enzyme of an imine reductase mutant enzyme and the glucose dehydrogenase. The co-expressed enzyme is used to prepare (S)-nicotine, and the cost of enzymatically preparing (S)-nicotine is lowered.

公开(公告)号：EP4353820A1

公开(公告)日：2024-04-17

申请号：EP22820531.6

申请日：2022-06-07

申请人： CJ Cheiljedang Corporation

发明人： IM, Yeong Eun ,  HA, Cheol Woong ,  YANG, Eun Bin ,  KIM, Yeonsoo

IPC分类号： C12N9/02 ,  C12N15/81 ,  C12P21/02

CPC分类号： C12N9/0004 ,  C12P21/02 ,  C12N15/81

摘要： The present application relates to a superoxide dismutase 1 variant and a method for producing glutathione or derivatives thereof using the same.

公开(公告)号：EP2998393B1

公开(公告)日：2018-03-07

申请号：EP15179791.7

申请日：2009-05-18

申请人： REG Life Sciences, LLC

发明人： Schirmer, Andreas ,  Brubaker, Shane ,  RUDE, Mathew

IPC分类号： C12N9/02 ,  C12N9/88 ,  C12P7/64 ,  C10L1/02 ,  C12P5/02 ,  C12P7/24 ,  C12P7/04

CPC分类号： C12P7/64 ,  C10L1/02 ,  C10L2200/0469 ,  C10L2290/26 ,  C12N9/0004 ,  C12N9/0008 ,  C12N9/88 ,  C12P5/02 ,  C12P5/026 ,  C12P7/04 ,  C12P7/24 ,  C12P7/6409 ,  C12P7/6436 ,  C12P7/649 ,  C12Y102/0108 ,  C12Y401/99005 ,  Y02E50/13 ,  Y02P20/52 ,  Y02T50/678

摘要： Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels.

公开(公告)号：EP2022852B8

公开(公告)日：2017-08-23

申请号：EP07744240.8

申请日：2007-05-28

申请人： Kaneka Corporation

发明人： KAWANO, Shigeru ,  ITO, Noriyuki ,  YASOHARA, Yoshihiko

IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12P13/00 ,  C12P17/10

CPC分类号： C12P13/008 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1096 ,  C12P13/001 ,  C12P13/002 ,  C12P17/10

公开(公告)号：EP3106522A4

公开(公告)日：2017-08-16

申请号：EP15746275

申请日：2015-02-09

申请人： JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECH

发明人： OHTA YUKARI ,  HATADA YUJI ,  HASEGAWA RYOICHI ,  NISHI SHINRO

IPC分类号： C12P7/22 ,  C12N1/20 ,  C12N9/02 ,  C12N15/09 ,  C12P7/24

CPC分类号： C12P7/22 ,  C12N1/20 ,  C12N9/0004 ,  C12N9/13 ,  C12P7/24 ,  C12P7/26 ,  C12R1/01

摘要： The object of the invention is to provide a method which, if compared with prior art, more specifically and efficiently produces a compound having a phenol propane structure from natural biomass containing lignins by causing enzymes to act on the biomass. The object is achieved by a method for producing a phenyl propane-based compound comprising a step of producing a phenyl propane-based compound by causing enzymes derived from microorganisms of the genus Novosphingobium to act on biomass containing lignins and/or lignin-related substances in the presence of NAD and reduction type glutathione.

摘要翻译： 本发明的目的是提供一种方法，如果与现有技术相比，通过使酶作用于生物质上，从含有木质素的天然生物质更具体且高效地生产具有苯酚丙烷结构的化合物。 该目的通过用于制备苯基丙烷类化合物的方法来实现，所述方法包括通过使源自Novosphingobium属的微生物的酶作用于含有木质素和/或木质素相关物质的生物质来制备苯基丙烷类化合物的步骤 NAD和还原型谷胱甘肽的存在。

公开(公告)号：EP3181687A1

公开(公告)日：2017-06-21

申请号：EP17154777.1

申请日：2010-05-03

申请人： Promega Corporation

发明人： Encell, Lance P. ,  Wood, Keith V. ,  Wood, Monika G. ,  Hall, Mary ,  Otto, Paul ,  Vidugiris, Gediminas ,  Zimmerman, Kristopher

IPC分类号： C12N9/02 ,  C12Q1/66

CPC分类号： C12Q1/66 ,  C12N9/0004

摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

摘要翻译： 本发明公开了修饰的萤光素酶多肽。 修饰的萤光素酶多肽与野生型Oplophorus萤光素酶具有至少60％的氨基酸序列同一性，并且在对应于SEQ ID NO：1的野生型Oplophorus萤光素酶中的氨基酸的位置上包含至少一个氨基酸取代。 相对于野生型Oplophorus萤光素酶，经修饰的萤光素酶多肽具有增强的发光，增强的信号稳定性和增强的蛋白质稳定性中的至少一种。