公开(公告)号：EP4419888A1

公开(公告)日：2024-08-28

申请号：EP22761945.9

申请日：2022-08-17

申请人： Beckman Coulter Biotechnology (Suzhou) Co., Ltd.

发明人： LIU, Lifeng ,  WU, Fei ,  SHI, Liang ,  TANG, Linqun

IPC分类号： G01N15/14

CPC分类号： B01L13/02 ,  G01N15/1425 ,  B01L2200/14120130101

公开(公告)号：EP4360759A1

公开(公告)日：2024-05-01

申请号：EP23168003.4

申请日：2023-04-14

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  YE, Guang-Ci

IPC分类号： B01L3/02 ,  B01L3/00 ,  G01N1/30 ,  G01N15/14 ,  G01N1/31 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N1/30 ,  G01N2015/001920130101 ,  G01N2015/100620130101 ,  G01N2015/141520130101 ,  G01N2015/14220130101 ,  G01N15/1404 ,  G01N2015/148120130101 ,  G01N15/1459 ,  G01N15/1425 ,  G01N15/1484 ,  G01N1/312 ,  B01L3/0268 ,  B01L3/5088 ,  B01L2400/08620130101 ,  G01N2015/01220240101 ,  G01N15/1433 ,  G01N15/1492

摘要： A biological particle enrichment apparatus (2) and a pico-droplet generator (21) thereof are provided. The pico-droplet generator (21) includes a container (211), a hollow needle (212) connected to the container (211), a first piezoelectric member (213) disposed on the container (211), and a second piezoelectric member (214) disposed on the hollow needle (212). The container (211) can receive a liquid specimen (200) having biological particles (201). The hollow needle (212) and the container (211) are fluid communicated with each other, and an inner diameter (D211) of the container (211) is within a range from 5 times to 30 times of an inner diameter (D212) of the hollow needle (212). The first piezoelectric member (213) is annularly disposed on a surrounding lateral side (2112) of the container (211), and enables the biological particles (201) in the container (211) to move along a direction away from the surrounding lateral side (2112) by vibrating the container (211). The second piezoelectric member (214) can squeeze the hollow needle (212), so that the liquid specimen (200) flows outwardly to form a pico-droplet (202).

公开(公告)号：EP3382373A1

公开(公告)日：2018-10-03

申请号：EP18164218.2

申请日：2018-03-27

申请人： SYSMEX CORPORATION

发明人： KINISHI, Motoi ,  KAMURA, Shohei ,  TATSUTANI, Hiroo ,  YOSHIOKA, Takashi ,  NAKAJIMA, Takayuki

IPC分类号： G01N15/14 ,  G01N35/00

CPC分类号： G01N15/1404 ,  G01N15/1425 ,  G01N15/1434 ,  G01N35/00871 ,  G01N2035/00891

摘要： A flow cytometer capable of easily and reliably providing flow cytometry results without requiring a special trained technician is provided.
The flow cytometer 10 includes an input unit 60 that receives an input of information for specifying a measurement item, a condition selection unit 71 for selecting a measurement condition from among one or a plurality of measurement conditions corresponding to information for specifying the measurement item received by the input unit 60, and a measurement unit 65 having a flow cell 20 through which a particle-containing liquid containing particles in the sample passes, wherein the measurement unit 65 optically measures particles of the particle-containing liquid flowing through the flow cell 20 in accordance with the measurement condition selected by the condition selection unit 71.

公开(公告)号：EP3220129A1

公开(公告)日：2017-09-20

申请号：EP15858201.5

申请日：2015-11-10

申请人： Sysmex Corporation

发明人： MATSUI, Kunihiko ,  TAMURA, Akiko

IPC分类号： G01N15/14 ,  G01N21/64

CPC分类号： G01N15/1425 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N33/4915 ,  G01N33/493 ,  G01N2015/0065 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2201/0612 ,  G01N2201/0697 ,  H01S5/0428

摘要： A sample measuring apparatus 10 includes: a laser diode 16a that applies laser light to a measurement specimen prepared from a sample; a detection unit 120 that acquires optical information from a particle in the measurement specimen to which the laser light is applied; a drive circuit 13 that supplies a direct-current drive signal to the laser diode 16a; and a high-frequency conversion circuit 14 that generates a potential that switches between a high level and a low level in a predetermined cycle to guide the drive signal outputted from the drive circuit 13 to a second signal path 103 which is different from a first signal path 102 connected to the laser diode16a in the predetermined cycle, thereby converting the drive signal to be supplied to the laser diode 16a into a high-frequency signal.

摘要翻译： 样本测量设备10包括：激光二极管16a，其将激光施加到由样本制备的测量样本; 检测单元120，其从被施加激光的测定试样中的微粒取得光学信息; 向激光二极管16a供给直流驱动信号的驱动电路13， 以及产生在预定周期中在高电平和低电平之间切换的电势以将从驱动电路13输出的驱动信号引导到不同于第一信号的第二信号路径103的高频转换电路14 路径102以预定周期连接到激光二极管16a，由此将要提供给激光二极管16a的驱动信号转换成高频信号。

公开(公告)号：EP2950079A1

公开(公告)日：2015-12-02

申请号：EP13872550.2

申请日：2013-11-19

申请人： Sony Corporation

发明人： OTSUKA Fumitaka ,  MURAKI Yosuke

IPC分类号： G01N15/14

CPC分类号： G01N15/1425 ,  B07C5/3422 ,  G01N15/1427 ,  G01N15/1459 ,  G01N15/1463 ,  G01N21/27 ,  G01N2015/1006 ,  G01N2015/1406 ,  G01N2015/1481 ,  G01N2015/149

摘要： Provided are a microparticle sorting device, and a method and a program for sorting microparticles capable of stabilizing sorting performance over a prolonged period of time.
The microparticle sorting device includes an imaging element and a controller. The imaging element obtains an image of fluid and fluid droplets at a position where the fluid discharged from an orifice which generates a fluid stream is converted into the fluid droplets. The controller controls driving voltage of an oscillation element which gives oscillation to the orifice and/or controls a position of the imaging element based on a state of the fluid in the image and/or a state of a satellite fluid droplet. The satellite fluid droplet does not include microparticles and exists between the position, where the fluid is converted into the fluid droplets, and a fluid droplet, among fluid droplets including the microparticles, which is closest to the position where the fluid is converted into the fluid droplets.

摘要翻译： 本发明提供一种微粒分选装置以及能够长期稳定分选性能的微粒分选方法及程序。 微粒分拣装置包括成像元件和控制器。 成像元件在从产生流体流的孔口排出的流体被转换成流体液滴的位置处获得流体和流体液滴的图像。 控制器基于图像中的流体的状态和/或卫星流体液滴的状态来控制振荡元件的驱动电压，该振荡元件给出节流孔振荡和/或控制成像元件的位置。 卫星流体液滴不包括微粒，并且存在于流体转变成流体液滴的位置和包含微粒的流体液滴之中的流体液滴之间，流体液滴最接近流体转换成流体的位置 液滴。

公开(公告)号：EP2053381A3

公开(公告)日：2014-10-22

申请号：EP08167349.3

申请日：2008-10-23

申请人： Sony Corporation

发明人： Shinoda, Masataka ,  Imanishi, Shingo

IPC分类号： G01N15/14 ,  G01N15/10

CPC分类号： G01N15/1425 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1438

摘要： Disclosed herein is an optical detection method and optical detection apparatus, the apparatus including: a light irradiation section (4) configured to irradiate a laser beam (5) upon one of fine particles (3) which are successively fed in a flow path (2); and a light detection section (9) configured to detect fluorescent light and/or scattered light (10) generated from any of the fine particles (3) upon which the laser beam (5) is irradiated; the method including the steps of: irradiating a laser beam (5) upon one of fine particles (3) which are successively fed in a flow path (2); and detecting fluorescent light and/or scattered light (10) generated from the fine particle (3); wherein the laser beam (5) being formed as a pulse laser beam whose pulse intensity is modulated such that one laser beam or two or more laser beams (5) having different wavelengths are irradiated by a plural number of times upon one fine particle (3) with the intensity varied.

公开(公告)号：EP2717035A2

公开(公告)日：2014-04-09

申请号：EP13004627.9

申请日：2013-09-24

申请人： Palas GmbH Partikel-und Lasermesstechnik

发明人： Weiß, Maximilian ,  Mölter, Leander

IPC分类号： G01N21/27 ,  G01N21/53

CPC分类号： G01J3/18 ,  G01N15/1425 ,  G01N15/1459 ,  G01N21/274 ,  G01N21/53 ,  G01N2015/0046

摘要： Zur Erfassung einer Dekalibrierung einer Vorrichtung zum Untersuchen kleiner Partikel sieht die Erfindung ein Verfahren vor, bei dem die Partikel (5) mit Licht (4) bestrahlt werden, von den Partikeln gestreutes Licht (6) detektiert (8), das gewonnene elektrische Signal verstärkt (9), digitalisiert (10) und entsprechend der die Partikelgröße repräsentierenden Intensität in einer Vielzahl von digitalen Kanälen erfasst wird und darüber hinaus, dass das Auftreten des Mie-Peaks in der gemessenen größenabhängigen Häufigkeitsverteilung überwacht wird und bei Abweichung des Mie-Peaks in einem anderen als dem ihm aufgrund der Messeinstellungen zukommenden digitalen Normkanal eine Meldung ausgegeben wird.
Hierzu ist bei einer Vorrichtung zur Erfassung der Konzentration kleiner Partikel in Gas, mit einem Proberohr (1), einer dieses bestrahlenden Lichtquelle (2), einem Detektor (8) zur Erfassung von an den Teilchen (5) gestreutem Streulicht (6), einem Analogverstärker (9), einem Analog-Digital-Wandler (10), einer Auswerteelektronik (11) und einer Anzeige- und Bedieneinheit (12), die Auswertelektronik zur Erfassung eines Mie-Peaks in der gemessenen Partikelanzahlverteilung und Zuordnung zu einem digitalen Erfassungskanal ausgebildet.

摘要翻译： 该方法包括用光照射颗粒（5）并检测由颗粒散射的光（6）。 一组数字通道中的电信号被放大，数字化并且被检测为对应于表示粒度的强度的测量的依赖于尺寸的频率分布。 监测测量尺寸依赖频率分布中峰值的出现，如果峰值出现在基于测量设置的峰值标准通道以外的数字通道中，峰值对应于米氏峰值，则提供报告。 还包括用于分析气体中的小颗粒的装置的独立权利要求。

公开(公告)号：EP2693192A1

公开(公告)日：2014-02-05

申请号：EP13769335.4

申请日：2013-02-04

申请人： Sony Corporation

发明人： HASHIMOTO Gakuji ,  TSUJI Akiko ,  MURAKI Yosuke ,  HATAMOTO Kouhei

IPC分类号： G01N15/14 ,  G01N21/27

CPC分类号： G01N15/1425 ,  B01L3/502761 ,  G01N15/14 ,  G01N15/1459 ,  G01N2015/149 ,  Y10S209/908

摘要： A microparticle sorting device that automatically and accurately adjusts the positions of a fluid stream and a collection container is provided.
The microparticle sorting device including a pair of deflecting plates that face each other with a passage area of a fluid stream therebetween, a camera that captures the image of the fluid stream, and a fluid stream detection light source that emits light parallel to a direction in which the deflecting plates face each other and that is movable in a direction perpendicular to the fluid stream and the light is provided. In the microparticle sorting device, the collection container that receives the fluid stream is mounted so as to be movable in the direction perpendicular to the fluid stream and the light.

摘要翻译： 的微粒分选装置并自动地和精确地设定bestimmt流体流，并且设置一个收集容器的位置。 包括一对偏转板并面对海誓山盟与流体流的通路面积在它们之间，一个照相机那样的微粒分选装置捕获的流体流的图像，以及流体流检测用光源没有发射光平行的方向上 其中偏转板面对海誓山盟并做垂直于流体流是在一个方向上移动，被设置在光。 在该微粒分选装置中，收集容器确实接收流体流被安装以便可在垂直的方向上的流体流和光移动。

公开(公告)号：EP2606337A2

公开(公告)日：2013-06-26

申请号：EP11818894.5

申请日：2011-08-22

申请人： Stratedigm Inc.

发明人： JAVADI, Shervin ,  KARUNARATNE, Arjuna

IPC分类号： G01N21/39 ,  G01N21/64

CPC分类号： G01N15/1434 ,  G01N15/1425 ,  G01N15/1459 ,  G01N2015/1438

摘要： Systems and methods for regarding a cytometry system are disclosed. The system can include a plurality lasers which are spatially separated from each other. Each laser can be assigned to a single detector. The single detector can process multiple events from each laser by digital switching of signal processing circuitry. Additional detectors can be assigned to receive light in a similar manner.

公开(公告)号：EP2182050A2

公开(公告)日：2010-05-05

申请号：EP09174448.2

申请日：2009-10-29

申请人： SYSMEX CORPORATION

发明人： Fujimoto, Yukie

IPC分类号： C12M1/34

CPC分类号： G01N33/56911 ,  G01N15/1425 ,  G01N15/1459 ,  G01N33/493 ,  G01N2015/1486 ,  Y10S435/808

摘要： A bacteria analyzer comprising: a sample preparing section for preparing a measurement sample from a specimen; a detector for detecting bacteria contained in the measurement sample prepared by the sample preparing section; and a controller including a memory under control of a processor, the memory storing instructions enabling the processor to carry out operations, comprising: obtaining the distribution state of bacteria detected by the detector; setting a bacteria count region according to the obtained bacteria distribution state; and counting the number of bacteria contained in the set bacteria count region is disclosed.
Method for analyzing bacteria, and a computer program product for bacteria analyzer are also disclosed.

摘要翻译： 1.一种细菌分析装置，其特征在于，包括：样本制备部，其从样本制备测定样品; 用于检测由样品制备部件制备的测量样品中所含的细菌的检测器; 以及控制器，其包括处理器控制下的存储器，所述存储器存储使所述处理器执行操作的指令，包括：获得由所述检测器检测到的细菌的分布状态; 根据获得的细菌分布状态设置细菌数区域; 并且公开了计数细菌计数区域中包含的细菌数量。 还公开了细菌分析方法和细菌分析仪的计算机程序产品。