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    Novel reagents for transfection of eukaryotic cells
    2.
    发明公开
    Novel reagents for transfection of eukaryotic cells 有权
    Neuartige Reagenzien zur Transfektion von eukaryotischen Zellen

    公开(公告)号：EP2604255A1

    公开(公告)日：2013-06-19

    申请号：EP12008260.7

    申请日：2006-05-17

    申请人： Molecular Transfer, Inc.

    发明人： Jessee, Joel ,  Gebeyehu, Gulilat

    IPC分类号： A61K9/127 ,  C12N15/88

    CPC分类号： A61K9/1272 ,  C12N15/88 ,  C12N2720/12022 ,  C12N2810/6063

    摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic o lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

    摘要翻译： 提供了将大分子递送到真核细胞中的组合物和方法。 来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。 这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。 还提供了新的阳离子型o脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。 脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

    NOVEL REAGENTS FOR TRANSFECTION OF EUKARYOTIC CELLS
    5.
    发明公开
    NOVEL REAGENTS FOR TRANSFECTION OF EUKARYOTIC CELLS 有权
    新试剂做了真核细胞的转染

    公开(公告)号：EP2015780A2

    公开(公告)日：2009-01-21

    申请号：EP06784437.3

    申请日：2006-05-17

    申请人： Molecular Transfer, Inc.

    发明人： JESSEE, Joel ,  GEBEYEHU, Gulilat

    IPC分类号： A61K48/00

    CPC分类号： A61K9/1272 ,  C12N15/88 ,  C12N2720/12022 ,  C12N2810/6063

    摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

    READY-TO-USE ELECTROPORATION CUVETTE INCLUDING FROZEN ELECTROCOMPETENT CELLS
    6.
    发明公开
    READY-TO-USE ELECTROPORATION CUVETTE INCLUDING FROZEN ELECTROCOMPETENT CELLS 审中-公开
    冷冻电感受细胞含READY电

    公开(公告)号：EP1727889A1

    公开(公告)日：2006-12-06

    申请号：EP05725836.0

    申请日：2005-03-18

    申请人： Molecular Transfer, Inc.

    发明人： BEBEE, Robert, L.

    IPC分类号： C12M1/42 ,  C12N13/00

    CPC分类号： C12M35/02

    摘要： A ready-to-use electroporation cuvette is provided that includes a cuvette, first and second electrodes positioned within the cuvette and electroporation competent cells frozen in a suspension solution within the cuvette, wherein the electroporation cuvette is configured to permit electroporation of the cells when the cells are thawed. The electroporation cuvette may be sealed with a cap that may be color coded to aid the user.

    AGENTS FOR IMPROVED DELIVERY OF NUCLEIC ACIDS TO EUKARYOTIC CELLS
    10.
    发明公开
    AGENTS FOR IMPROVED DELIVERY OF NUCLEIC ACIDS TO EUKARYOTIC CELLS 有权
    用于改善向真核细胞递送核酸的药剂

    公开(公告)号：EP2696678A1

    公开(公告)日：2014-02-19

    申请号：EP12771294.1

    申请日：2012-04-16

    申请人： Molecular Transfer, Inc.

    发明人： JESSEE, Joel, A. ,  GEBEYEHU, Gulilat

    IPC分类号： A01N37/12 ,  A01N37/44 ,  A61K31/195

    摘要： New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.

    摘要翻译： 提供了用于将诸如核酸的大分子递送到真核细胞中的新型阳离子脂质。 脂质可以单独使用，与其他脂质组合使用和/或与其他转染增强剂组合使用以制备转染复合物。