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    METHOD AND KIT FOR DIAGNOSTING IRRITABLE BOWEL SYNDROME AND FOR ITS RELIEF THROUGH DIETARY INTERVENTIONS
    5.
    发明公开
    METHOD AND KIT FOR DIAGNOSTING IRRITABLE BOWEL SYNDROME AND FOR ITS RELIEF THROUGH DIETARY INTERVENTIONS 审中-公开

    公开(公告)号：EP3913371A1

    公开(公告)日：2021-11-24

    申请号：EP21169908.7

    申请日：2021-04-22

    申请人： Neuroimmun GmbH

    发明人： KELLNER, Karl-Heinz

    IPC分类号： G01N33/68 ,  G01N33/94

    摘要： Method and kit for diagnosis of irritable bowel syndrome and irritable colon, comprising: a stool extraction system for preparation of a stool extract of substances of the gut-brain-axis, a derivatizing reagent for small biogenic amines; antibodies specific for derivatized tryptophan, serotonin, and GABA; and immunoassay reagents and buffers for combined determination of tryptophan, serotonin, and GABA. The method of in vitro diagnosis of irritable bowel syndrome (IBS) or irritable colon comprises a collecting a specimen of fresh stool; a transfer of the specimen into a stabilizing buffer; an extraction of the biogenic amines (neurotransmitters, neuroactive substances and tissue-active substances) and substances of the gut-brain axis; a combined quantitative analysis for tryptophan, serotonin and GABA, and optionally of histamine and kynurenic acid, to determine any imbalance of substances of the gut-brain-axis and for in vitro diagnosis of irritable bowel syndrome and/or irritable colon based on a comparison respective values and ranges in stools of healthy subjects.

    METHOD AND KIT OF PARTS FOR EXTRACTION OF NUCLEIC ACIDS
    10.
    发明授权
    METHOD AND KIT OF PARTS FOR EXTRACTION OF NUCLEIC ACIDS 有权
    用于提取核酸的部件的方法和试剂盒

    公开(公告)号：EP3114224B1

    公开(公告)日：2018-05-09

    申请号：EP15712533.7

    申请日：2015-03-03

    申请人： IFP Privates Institut für Produktqualität GmbH

    发明人： WEBER, Wolfgang ,  WERNER, Christine

    IPC分类号： C12N15/10

    CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  C12Q1/689

    摘要： Composition, method and kit of parts for a simplified and safe extraction of nucleic acids from biological samples, such as crude and processed food, and subsequent analysis by polymerase chain reaction to the presence of animal material, genetically modified organisms, allergens and pathogens. The method comprises the addition of extracting composition together with hot water for extraction and stabilization of nucleic acids as well as removal of substances interfering with DNA polymerase activity.