公开(公告)号：EP1962093A1

公开(公告)日：2008-08-27

申请号：EP06833421.8

申请日：2006-11-27

申请人： Japan Science and Technology Agency ,  Japan Advanced Institute of Science and Technology ,  Bio Device Technologies Co., Ltd.

发明人： NAGATANI, Naoki ,  YUI, Teruko ,  TAMIYA, Eiichi ,  ENDOU, Tatsurou ,  TANAKA, Ryou

IPC分类号： G01N33/543

CPC分类号： G01N33/54393 ,  Y10S435/97

摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR
SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.

摘要翻译： [问题]为了减少例如捕获抗体的使用量而不降低检测灵敏度。 同时，即使在仅积聚少量标记抗体的情况下，也能够在判定区域中实现强烈的显色或发光。 降低夹心法中的检测限。 在比赛方法中扩大动态范围。 解决问题的手段本发明提供一种使用受试物质通过免疫学分析方法分析受试物质的方法，具有测定区域的载体，其上选自能够与测试物质特异性结合的捕获抗体和 已经固定了捕获能够与被检物质特异性结合的抗原，以及能够与被检物质特异性结合的标记抗体，其中具有增敏作用的标记已经被固定在支持物的测定区域上。

公开(公告)号：EP1962093B1

公开(公告)日：2013-06-12

申请号：EP06833421.8

申请日：2006-11-27

申请人： Japan Science and Technology Agency ,  Japan Advanced Institute of Science and Technology ,  Bio Device Technologies Co., Ltd.

发明人： NAGATANI, Naoki ,  YUI, Teruko ,  TAMIYA, Eiichi ,  ENDOU, Tatsurou c/o Tokyo Institute of Technology ,  TANAKA, Ryou

IPC分类号： G01N33/543

CPC分类号： G01N33/54393 ,  Y10S435/97

公开(公告)号：EP1962093A4

公开(公告)日：2009-04-29

申请号：EP06833421

申请日：2006-11-27

申请人： JAPAN SCIENCE & TECH AGENCY ,  JAPAN ADV INST SCIENCE & TECH ,  BIO DEVICE TECHNOLOGIES CO LTD

发明人： NAGATANI NAOKI ,  YUI TERUKO ,  TAMIYA EIICHI ,  ENDOU TATSUROU ,  TANAKA RYOU

IPC分类号： G01N33/543

CPC分类号： G01N33/54393 ,  Y10S435/97

摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.