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公开(公告)号：EP4349958A1
公开(公告)日：2024-04-10
申请号：EP22811305.6
申请日：2022-05-24
IPC分类号： C12N1/00 , B01D61/14 , B01D61/58 , B01D63/02 , B01D69/00 , B01D69/02 , B01D69/08 , C07K1/34 , C12M1/12 , C12N5/0775
摘要： [Object]
To provide an isolation and purification method for isolating and purifying extracellular vesicles.
[Solution]
An isolation and purification method of extracellular vesicles is disclosed. A hollow fiber membrane has an inner diameter of 0.2 mm to 1.4 mm and a molecular weight cut-off of 100000 to 1000000. Filtering includes a first filtration process of press-fitting the culture supernatant of the mesenchymal stem cells from a first opening on one end side of the hollow fiber membrane and filtering the culture supernatant to separate the culture supernatant into a permeate and a first concentrate, and a second filtration process of press-fitting the first concentrate from a second opening on the other end side of the hollow fiber membrane and filtering the first concentrate to separate the first concentrate into a permeate and a second concentrate. A concentrate is produced in which a concentration of the extracellular vesicles is increased by alternating tangential flow filtration in which the first filtration process and the second filtration process are alternately performed a plurality of times. A membrane surface velocity in the first filtration process and the second filtration process is 0.3 m/sec to 2 m/sec.-
2.发明公开
公开(公告)号：EP1707538A1
公开(公告)日：2006-10-04
申请号：EP05703889.5
申请日：2005-01-20
发明人： MURAMOTO, Takahisa , YOSHIOKA, Yoshiyuki , MORIMITSU, Kozo , MACHIDA, Masashi , OGOSHI, Shingo , NISHII, Masahiro , MIYAZAKI, Yasumitsu , KAMEDA, Shigeru
CPC分类号： B01D61/02 , B01D61/022 , B01D61/04 , B01D61/14 , B01D61/142 , B01D61/16 , B01D61/58 , B01D65/02 , B01D2311/04 , B01D2317/025 , B01D2317/08 , B01D2321/04 , C02F1/28 , C02F1/44 , C02F1/722 , C02F9/00 , C02F2101/12 , C02F2101/305 , C02F2101/327 , C02F2101/36 , C02F2101/366 , C02F2303/16 , B01D2311/2626
摘要： Provided a method for treating hardly-decomposable-substance-containing water, in which hardly decomposable substances such as dioxins, contained in contaminated water (treatment raw water) are concentrated and rendered harmless by providing the steps of (B) adding an adsorbent to water containing a hardly decomposable substance (treatment raw water) to cause the hardly decomposable substance to be adsorbed on the adsorbent (adsorption treatment step), (C) separating a permeated liquid through a filter membrane to concentrate the adsorbent adsorbing the hardly decomposable substance (membrane filtering treatment step), and (D) chemically decomposing the hardly decomposable substance adsorbed on the concentrated adsorbent with a peroxide without any operation of desorption from the adsorbent (chemical decomposition step), and the method can be applied to water containing a reducing substance such as bisulfate that neutralize free chlorine and can render the hardly decomposable substances harmless efficiently at a low cost without being limited by properties of the hardly decomposable substances contained.
摘要翻译： 提供了一种用于处理水含有难分解性物质 - ，其中难分解的物质：如二恶英，包含在污染的水（处理原水）中浓缩，并通过提供的增加与吸附剂对水的步骤（B）变成无害 含有难分解物质（处理原水），以使难分解物质被吸附在吸附剂（吸附处理步骤），（C）通过过滤器膜分离的渗透液以浓缩的吸附剂吸附难分解物质（膜 滤波处理工序），和（D）化学分解上用过氧化物浓缩的吸附剂吸附，而不从吸附剂（化学分解步骤解吸任何手术的难分解物质），并且该方法可应用于水含有还原物质的搜索 硫酸氢做中和游离氯，可以使难分解的物质 s的低成本高效无害而不受包含在难分解物质的性质的限制。
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公开(公告)号：EP4349849A1
公开(公告)日：2024-04-10
申请号：EP22811306.4
申请日：2022-05-24
发明人： NAKATSUKA Shuji
摘要： [Object] To provide a method for isolating and purifying a minute useful substance. [Solution] Disclosed is a method for isolating and purifying a minute useful substance. The method includes filtering a liquid containing a minute useful substance through a hollow fiber membrane. The hollow fiber membrane has an inner diameter of 0.2 mm to 1.4 mm and a molecular weight cut-off of 100000 to 1000000. The filtering includes a first filtration process of press-fitting the liquid containing the minute useful substance from a first opening on one end side of the hollow fiber membrane and filtering the liquid to separate the liquid into a permeate and a first concentrate, and a second filtration process of press-fitting the first concentrate from a second opening on the other end side of the hollow fiber membrane and filtering the first concentrate to separate the first concentrate into a permeate and a second concentrate. A concentrate is produced in which a concentration of the minute useful substance is increased by filtration in which the first filtration process and the second filtration process are alternately performed a plurality of times at a membrane surface velocity of 0.3 m/sec to 2 m/sec.
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公开(公告)号：EP3854430A1
公开(公告)日：2021-07-28
申请号：EP19862019.7
申请日：2019-09-18
摘要： A hollow fiber porous membrane including polyethersulfone or polysulfone, wherein the hollow fiber porous membrane has an inner diameter from 300 to 600 µm, a thickness from 70 to 200 µm, a molecular weight cut-off of 10000 or lower, and a plurality of pores having a pore diameter from 0.1 to 0.5 µm throughout an outer surface; and a swelling rate of less than 5% as defined below: Swelling Rate (%): for 20 or more of the hollow fiber porous membranes, after a membrane thickness in a cross section of each one of the hollow fiber porous membranes in the width direction is measured at randomly selected 10 or more locations, an average membrane thickness is calculated based on 200 or more locations in total, and the swelling rate is calculated by a formula below: Swelling Rate (%) = (location numbers where the membrane thickness as measured exceeded 1.3 times the average membrane thickness)/(membrane thickness measurement numbers) x 100
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5.发明公开
公开(公告)号：EP1707538A4
公开(公告)日：2008-05-21
申请号：EP05703889
申请日：2005-01-20
发明人： MURAMOTO TAKAHISA , YOSHIOKA YOSHIYUKI , MORIMITSU KOZO , MACHIDA MASASHI , OGOSHI SHINGO , NISHII MASAHIRO , MIYAZAKI YASUMITSU , KAMEDA SHIGERU
IPC分类号： C02F1/28 , B01D61/02 , B01D61/04 , B01D61/14 , B01D61/16 , B01D61/58 , B01D65/02 , C02F1/44 , C02F1/52 , C02F1/72 , C02F9/00 , C02F101/32 , C02F101/36
CPC分类号： B01D61/02 , B01D61/022 , B01D61/04 , B01D61/14 , B01D61/142 , B01D61/16 , B01D61/58 , B01D65/02 , B01D2311/04 , B01D2317/025 , B01D2317/08 , B01D2321/04 , C02F1/28 , C02F1/44 , C02F1/722 , C02F9/00 , C02F2101/12 , C02F2101/305 , C02F2101/327 , C02F2101/36 , C02F2101/366 , C02F2303/16 , B01D2311/2626
摘要： Provided a method for treating hardly-decomposable-substance-containing water, in which hardly decomposable substances such as dioxins, contained in contaminated water (treatment raw water) are concentrated and rendered harmless by providing the steps of (B) adding an adsorbent to water containing a hardly decomposable substance (treatment raw water) to cause the hardly decomposable substance to be adsorbed on the adsorbent (adsorption treatment step), (C) separating a permeated liquid through a filter membrane to concentrate the adsorbent adsorbing the hardly decomposable substance (membrane filtering treatment step), and (D) chemically decomposing the hardly decomposable substance adsorbed on the concentrated adsorbent with a peroxide without any operation of desorption from the adsorbent (chemical decomposition step), and the method can be applied to water containing a reducing substance such as bisulfate that neutralize free chlorine and can render the hardly decomposable substances harmless efficiently at a low cost without being limited by properties of the hardly decomposable substances contained.
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