公开(公告)号：EP0975794A4

公开(公告)日：2002-12-04

申请号：EP98914468

申请日：1998-04-10

申请人： GILVARG CHARLES

发明人： GILVARG CHARLES

IPC分类号： C12Q1/34 ,  C12Q1/37 ,  G01N33/574 ,  C12Q1/00

CPC分类号： C12Q1/37 ,  G01N33/57438 ,  Y10S435/963 ,  Y10S435/968

摘要： Methods of enhancing sensitivity and specificity of an assay measuring enzymatic activity in a sample by measuring enzymatic activity in the sample in the presence and absence of a specific inhibitor of the enzymatic activity are provided. Methods of measuring carboxypeptidase A levels and total carboxypeptidase A levels, wherein procarboxypeptidase A is converted to carboxypeptidase A by addition of clostripain, in a biological fluid with a carboxypeptidase A substrate, specificity of which is enhanced by addition of a carboxypeptidase A specific inhibitor are also provided. In addition, methods of diagnosing acute pancreatitis by measurement of carboxypeptidase A levels and pancreatic cancer by measurement of total carboxypeptidase A levels are also provided.

公开(公告)号：EP0975794B1

公开(公告)日：2006-02-22

申请号：EP98914468.8

申请日：1998-04-10

申请人： Gilvarg, Charles

发明人： Gilvarg, Charles

IPC分类号： C12Q1/37 ,  C12Q1/00

CPC分类号： C12Q1/37 ,  G01N33/57438 ,  Y10S435/963 ,  Y10S435/968

摘要： Methods of enhancing sensitivity and specificity of an assay measuring enzymatic activity in a sample by measuring enzymatic activity in the sample in the presence and absence of a specific inhibitor of the enzymatic activity are provided. Methods of measuring carboxypeptidase A levels and total carboxypeptidase A levels, wherein procarboxypeptidase A is converted to carboxypeptidase A by addition of clostripain, in a biological fluid with a carboxypeptidase A substrate, specificity of which is enhanced by addition of a carboxypeptidase A specific inhibitor are also provided. In addition, methods of diagnosing acute pancreatitis by measurement of carboxypeptidase A levels and pancreatic cancer by measurement of total carboxypeptidase A levels are also provided.