利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

156 条记录 (耗时 0.024 秒)

    METHOD OF DETECTING PROCARBOXYPEPTIDASE A AND CARBOXYPEPTIDASE A LEVELS IN BIOLOGICAL FLUIDS
    5.
    发明公开
    METHOD OF DETECTING PROCARBOXYPEPTIDASE A AND CARBOXYPEPTIDASE A LEVELS IN BIOLOGICAL FLUIDS 失效
    程序用于确定PROCARBOXYPETIDASE-A和羧一面镜子，生物液体

    公开(公告)号：EP0975794A4

    公开(公告)日：2002-12-04

    申请号：EP98914468

    申请日：1998-04-10

    申请人： GILVARG CHARLES

    发明人： GILVARG CHARLES

    IPC分类号： C12Q1/34 ,  C12Q1/37 ,  G01N33/574 ,  C12Q1/00

    CPC分类号： C12Q1/37 ,  G01N33/57438 ,  Y10S435/963 ,  Y10S435/968

    摘要： Methods of enhancing sensitivity and specificity of an assay measuring enzymatic activity in a sample by measuring enzymatic activity in the sample in the presence and absence of a specific inhibitor of the enzymatic activity are provided. Methods of measuring carboxypeptidase A levels and total carboxypeptidase A levels, wherein procarboxypeptidase A is converted to carboxypeptidase A by addition of clostripain, in a biological fluid with a carboxypeptidase A substrate, specificity of which is enhanced by addition of a carboxypeptidase A specific inhibitor are also provided. In addition, methods of diagnosing acute pancreatitis by measurement of carboxypeptidase A levels and pancreatic cancer by measurement of total carboxypeptidase A levels are also provided.

    Improved immunohistochemical staining method and reagents therefor
    7.
    发明公开
    Improved immunohistochemical staining method and reagents therefor 失效
    免疫组化免疫组化Färbeverfahrenund Reagenziendafür

    公开(公告)号：EP1030178A1

    公开(公告)日：2000-08-23

    申请号：EP00105355.2

    申请日：1991-02-27

    申请人： VENTANA MEDICAL SYSTEMS, INC.

    发明人： Miller, Phillip C. ,  Degroff, Michael J. ,  Gizinski, Michael J. ,  Rybski, James A. ,  Vandivort, Pamela S. ,  Hartman, Anthony L.

    IPC分类号： G01N33/531 ,  G01N33/535 ,  G01N33/543 ,  G01N1/30 ,  G01N33/567 ,  C12Q1/00 ,  C12Q1/68 ,  C12Q1/28 ,  C12N9/96 ,  G01N1/28

    CPC分类号： G01N33/535 ,  C12N9/96 ,  C12Q1/28 ,  C12Q2326/10 ,  G01N1/30 ,  G01N33/54393 ,  G01N33/567 ,  G01N33/581 ,  G01N2035/00079 ,  G01N2035/00277 ,  Y10S435/96 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S436/825 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/25125

    摘要： The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section) is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient to substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. Color development reagents, including, in a preferred embodiment, a stabilized diaminobenzidine (DAB) solution, are combined with the tissue section for a time sufficient for color development. Following rinsing, the tissue section is ready for analysis.

    摘要翻译： 本发明提供使用免疫化学试剂对载玻片进行染色的改进方法。 该方法包括以下步骤。 使用改进的包含水和洗涤剂的冲洗溶液洗涤载玻片的测定区域（含有组织切片的区域）。 将蒸发抑制剂液体施加到载玻片上以覆盖测定区域。 对于需要遮掩的抗原，组织切片与改进的稳定的蛋白水解酶溶液组合。 将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。 将含有与第二抗体相同物种的球蛋白的改良稀释液中的一抗与组织切片组合足以基本完成抗体结合的时间。 将载玻片冲洗，并重新施加蒸发抑制剂液体。 将改进的稀释剂中的标记的第二抗体与组织切片组合足够的时间以使基本上完全的抗体结合。 将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。 在优选的实施方案中，将显影剂（包括稳定的二氨基联苯胺（DAB）溶液）与组织切片结合足以进行显色的时间。 冲洗后，组织部分准备进行分析。

    Method for detecting marijuana in a hair sample
    10.
    发明授权
    Method for detecting marijuana in a hair sample 失效
    一种用于毛发样品中检测大麻方法

    公开(公告)号：EP0634014B1

    公开(公告)日：1999-12-29

    申请号：EP94907938.8

    申请日：1994-02-01

    申请人： PSYCHEMEDICS CORPORATION

    发明人： BAUMGARTNER, Werner A.

    IPC分类号： G01N33/94 ,  C12Q1/37

    CPC分类号： G01N1/405 ,  C12Q1/37 ,  G01N1/4044 ,  G01N33/53 ,  G01N33/94 ,  G01N33/948 ,  G01N2001/007 ,  G01N2333/4742 ,  G01N2333/96433 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S436/815 ,  Y10S436/825 ,  Y10S436/901 ,  Y10T436/25125 ,  Y10T436/25375

    摘要： A method for the direct analysis of analyte indicative of marijuana exposure found in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing dithiothreitol or dithioerythritol, a protease suitable for the digestion of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially digest the sample of keratin structure to form a digest solution, followed by mixing the digest solution with a suspension of an ion exchange resin to remove an interfering, cross reacting substance naturally found in hair and finally subjecting the digest solution to analysis to determine the identity and amount of marijuana analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample in order to deactivate the activator. The enzyme may be a protease with papain, chymopapain, and proteinase K being preferred for use in the invention. Exemplary ion exchange resins useful in the method according to the invention are DEAE Sephadex (Diethylaminoethyl Sephadex) and DEAE Sepharose (Diethylaminoethyl Sepharose).