公开(公告)号：EP1144692A1

公开(公告)日：2001-10-17

申请号：EP99973074.0

申请日：1999-11-30

申请人： Nycomed Pharma A/S ,  Grant, Anne Rosemary

发明人： IHLE, Oystein ,  MICHAELSEN, Terje, Einar ,  JOHNE, Berit

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/1006 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2563/131 ,  C12Q2525/131 ,  C12Q2522/101 ,  C12Q2563/119

摘要： A method for at least partially separating nucleic acid molecules in a sample into populations wherein a population is tagged or capable of being tagged with a moiety capable of being immobilised on a matrix, said method comprising contacting the nucleic acid containing sample with a matrix whereby the tagged molecules are captured by the matrix and thereby separated from untagged molecules. The method may be used in a variety of applications, including separating restriction enzyme digestion products, separating linear from circular nucleic acid molecules, in vitro packaging of bacteriophage particles, diagnostic PCR.