公开(公告)号：EP2998667B1

公开(公告)日：2020-11-25

申请号：EP14003226.9

申请日：2014-09-17

申请人： Hochschule Biberach

发明人： Becker, Martin ,  Köberle, Thomas

IPC分类号： F25B49/00 ,  F25B49/02

公开(公告)号：EP2937417A1

公开(公告)日：2015-10-28

申请号：EP14166041.5

申请日：2014-04-25

申请人： Hochschule Biberach

发明人： Otte, Kerstin ,  Handrick, René ,  Fischer, Simon

IPC分类号： C12N15/11 ,  C12N15/113 ,  C12N15/67 ,  C12N15/85

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2330/50

摘要： The invention relates to a nucleic acid construct comprising at least two different regions, wherein the regions are selected of a first region encoding for at least one miRNA stimulating cellular production of a biomolecule in a cell, a second region encoding for at least one miRNA and/or miRNA-inhibitor suppressing cell death, and a third region encoding for at least one miRNA and/or miRNA-inhibitor regulating cell proliferation. The invention further relates to a cell comprising a respective nucleic acid construct and to a method for increasing the yield of a biomolecule produced by a cell cultured in vitro comprising at least two steps selected of stimulating cellular production of the biomolecule, reducing cell death, and regulating proliferation.

摘要翻译： 本发明涉及包含至少两个不同区域的核酸构建体，其中所述区域选自编码细胞中生物分子的至少一种miRNA的至少一种miRNA的第一区域，编码至少一种miRNA的第二区域和 或抑制细胞死亡的miRNA抑制剂，以及编码调节细胞增殖的至少一种miRNA和/或miRNA抑制剂的第三区域。 本发明还涉及包含相应的核酸构建体的细胞和用于提高由体外培养的细胞产生的生物分子的产率的方法，所述细胞包含选自刺激细胞生成生物分子，减少细胞死亡的至少两个步骤，和 调节增殖。

公开(公告)号：EP2937417B1

公开(公告)日：2019-02-20

申请号：EP14166041.5

申请日：2014-04-25

申请人： Hochschule Biberach

发明人： Otte, Kerstin ,  Handrick, René ,  Fischer, Simon

IPC分类号： C12N15/11 ,  C12N15/113 ,  C12N15/67 ,  C12N15/85

公开(公告)号：EP3134525A2

公开(公告)日：2017-03-01

申请号：EP15719458.0

申请日：2015-04-24

申请人： Hochschule Biberach

发明人： FISCHER, Simon ,  HANDRICK, René ,  OTTE, Kerstin

IPC分类号： C12N15/11 ,  C12N15/113 ,  C12N15/67 ,  C12N15/85

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2330/50

摘要： The invention relates to a nucleic acid construct comprising at least two different regions, wherein the regions are selected of a first region encoding for at least one miRNA stimulating cellular production of a biomolecu!e in a cell, a second region encoding for at least one miRNA and/or miRNA-inhibitor suppressing cell death, and a third region encoding for at least one miRNA and/or miRNA-inhibitor regulating cell proliferation. The invention further relates to a cell comprising a respective nucleic acid construct and to a method for increasing the yield of a biomolecule produced by a cell cultured in vitro comprising at least two steps selected of stimulating cellular production of the biomolecule, reducing cell death, and regulating proliferation.

摘要翻译： 本发明涉及包含至少两个不同区域的核酸构建体，其中所述区域选自编码细胞中生物分子的至少一种miRNA的至少一种miRNA的第一区域，编码至少一种miRNA的第二区域和 或抑制细胞死亡的miRNA抑制剂，以及编码调节细胞增殖的至少一种miRNA和/或miRNA抑制剂的第三区域。 本发明还涉及包含相应的核酸构建体的细胞和用于提高由体外培养的细胞产生的生物分子的产率的方法，所述细胞包含选自刺激细胞生成生物分子，减少细胞死亡的至少两个步骤，和 调节增殖。

公开(公告)号：EP4092125A1

公开(公告)日：2022-11-23

申请号：EP21174141.8

申请日：2021-05-17

申请人： Hochschule Biberach

发明人： OTTE, Kerstin ,  HANDRICK, René ,  ZEH, Nikolas

IPC分类号： C12N15/85 ,  C07K1/00 ,  C07K14/47

摘要： Disclosed is a method for producing a biomolecule by a cell cultured in vitro, the method comprising the steps of: (a) propagating the cell in a cell culture, wherein the cell is transformed with a vector, wherein the vector comprises (i) at least one hypoxia-responsive element (HRE) consisting of a nucleic acid sequence selected from the group consisting of SEQ ID NO.: 1, SEQ ID NO.: 2, SEQ ID NO.: 3, SEQ ID NO.: 4 and functional variants of any one of these sequences; (ii) a promoter that is active in the cell, wherein the promoter is operably linked to the HRE; (iii) a nucleic acid sequence encoding the biomolecule, wherein the nucleic acid sequence is operably linked to the promoter; and (b) isolating the biomolecule from the cell culture. Further disclosed is a cell for producing a biomolecule in vitro.

公开(公告)号：EP2998667A1

公开(公告)日：2016-03-23

申请号：EP14003226.9

申请日：2014-09-17

申请人： Hochschule Biberach

发明人： Becker, Martin ,  Köberle, Thomas

IPC分类号： F25B49/00 ,  F25B49/02

CPC分类号： F25B49/005 ,  F25B49/02 ,  F25B2341/0653 ,  F25B2500/19 ,  F25B2700/191 ,  F25B2700/193 ,  F25B2700/195 ,  F25B2700/197 ,  F25B2700/21175 ,  Y02B30/72

摘要： In a method for evaluating the energy efficiency of a refrigeration machine (1; 2) and/or a heat pump (3; 4), wherein a working fluid of the refrigeration machine (1; 2) and/or heat pump is running through an expansion valve (40; 40A, 40B, 40C) at one point of a thermodynamic cycle, at different positions of the thermodynamic cycle, at least one thermodynamic parameter ( T entrance , p entrance , p exit , T superheat , p superheat , Tcompressor, p compressor ) of the working fluid is detected. The mass flow ( ṁ F ) of the working fluid is determined using a classification (m) of the opening of the expansion valve (40; 40A, 40B, 40C) and some of the detected thermodynamic parameters ( T entrance , p entrance , p exit ) of the working fluid. A cooling power and/or a heating power ( Q̇ ) of the refrigeration machine (1; 2) and/or heat pump (3; 4) is determined as a function of the mass flow ( ṁ F ) of the working fluid. The energy efficiency of the refrigeration machine and/or heat pump (3; 4) is evaluated by comparing the cooling power and/or heating power (Q) to an electrical power (P el ) used to power the refrigeration machine and/or heat pump.

摘要翻译： 在用于评价的制冷机器的能量效率的方法（1，2）和/或热泵（3; 4），worin制冷机的工作流体（1，2）和/或热泵，通过运行 上膨胀阀（40; 40A，40B，40C）在热力学循环中的一个点，在热力循环的不同位置，至少一个热力学参数（T入口，对入口，对出口，T过热，对过热，Tcompressor 时，工作流体的对压缩机）被检测到。 和一些所检测的热力学参数（T入口，对入口，P;工作流体的质量流量（米F）是使用膨胀阀（40A，40B，40C 40）的开口的分类（米）的确定性开采 工作流体的出口）。 冷却功率和/或冷冻机的加热功率（Q）（1; 2）和/或热泵（3; 4）被确定为工作流体的质量流量（米F）的函数。 制冷机器和/或热泵的能量效率（3; 4）通过在电功率（P EL）的冷却功率和/或热功率（Q）进行比较来评估用于将制冷机和/或热动力 泵。