公开(公告)号：EP0062434B1

公开(公告)日：1985-09-25

申请号：EP82301451.9

申请日：1982-03-19

申请人： Kiel, Jonathan L

发明人： Kiel, Jonathan L

IPC分类号： C12N11/18 ,  A61K37/50

CPC分类号： A61K38/44 ,  A61K9/0019 ,  A61K9/06 ,  A61K9/14 ,  A61K9/146 ,  A61K9/19 ,  A61K38/385 ,  A61K38/443 ,  A61K47/42 ,  C12N11/18 ,  C12Y101/03004 ,  C12Y111/01007 ,  A61K2300/00

公开(公告)号：EP0175577A3

公开(公告)日：1987-11-25

申请号：EP85306646

申请日：1985-09-18

申请人： Kiel, Jonathan L

发明人： Kiel, Jonathan L

IPC分类号： G01N33/52 ,  G01N33/533 ,  C12Q01/02 ,  C12Q01/32 ,  G01N33/569 ,  G01N33/576

CPC分类号： C12Q1/32 ,  G01N33/533 ,  G01N33/582

摘要： A composition for use in a thermochemiluminescent assay. The composition includes a heme protein, a luminescer such as luminol, and serum albumin, the luminescer being non-covalently bonded to serum albumin. The composition may be used in a thermochemiluminescent for the ligant of a specific binding pair in which the composition is contacted with a fluid sample suspected of containing the antiligand of the specific binding pair and the subsequent light-emitting reaction is quantified. The specific binding light-emitting reaction is quantified. The specific binding pair can be, for instance, an enzyme and the substrate for which it is specific. The composition and the assay can be modified to detect ionizing radiation, peroxidative injury to cells, heat or a particular cell type.

摘要翻译： 用于热化学发光测定的组合物。 组合物包括血红素蛋白，发光体如鲁米诺和血清白蛋白，发光体与血清白蛋白非共价结合。 组合物可用于特异性结合对的配偶体的热化学发光，其中组合物与怀疑含有特异性结合对的反配体的流体样品接触，并且随后的发光反应被定量。 对特异性结合发光反应进行定量。 特异性结合对可以是例如酶和其特异性的底物。 组合物和测定可以被修改以检测电离辐射，对细胞的过氧化损伤，热或特定的细胞类型。

公开(公告)号：EP0175577A2

公开(公告)日：1986-03-26

申请号：EP85306646.2

申请日：1985-09-18

申请人： Kiel, Jonathan L

发明人： Kiel, Jonathan L

IPC分类号： G01N33/52 ,  G01N33/533 ,  C12Q1/02 ,  C12Q1/32

CPC分类号： C12Q1/32 ,  G01N33/533 ,  G01N33/582

摘要： A composition for use in a thermochemiluminescent assay. The composition includes a heme protein, a luminescer such as luminol, and serum albumin, the luminescer being non-covalently bonded to serum albumin. The composition may be used in a thermochemiluminescent for the ligant of a specific binding pair in which the composition is contacted with a fluid sample suspected of containing the antiligand of the specific binding pair and the subsequent light-emitting reaction is quantified. The specific binding light-emitting reaction is quantified. The specific binding pair can be, for instance, an enzyme and the substrate for which it is specific. The composition and the assay can be modified to detect ionizing radiation, peroxidative injury to cells, heat or a particular cell type.

摘要翻译： 用于热化学发光测定的组合物。 该组合物包括血红素蛋白，诸如鲁米诺的发光体和血清白蛋白，该发光体与血清白蛋白非共价结合。 该组合物可以用于热化学发光，用于特异性结合对的配体，其中组合物与怀疑含有特异性结合对的抗配体的流体样品接触，并且随后的发光反应被定量。 量化特异性结合发光反应。 特异性结合对可以是例如酶和它特异性的底物。 可以对组合物和测定进行修改以检测电离辐射，对细胞的过氧化损伤，加热或特定细胞类型。

公开(公告)号：EP0062434A1

公开(公告)日：1982-10-13

申请号：EP82301451.9

申请日：1982-03-19

申请人： Kiel, Jonathan L

发明人： Kiel, Jonathan L

IPC分类号： C12N11/18 ,  A61K37/50

CPC分类号： A61K38/44 ,  A61K9/0019 ,  A61K9/06 ,  A61K9/14 ,  A61K9/146 ,  A61K9/19 ,  A61K38/385 ,  A61K38/443 ,  A61K47/42 ,  C12N11/18 ,  C12Y101/03004 ,  C12Y111/01007 ,  A61K2300/00

摘要： A therapeutic preparation, and a process of using the preparation, for the control of tumour growth and infectious diseases. The preparation is an immobilized cytotoxic enzyme system which is carried by an insoluble substrate that is administered into the mammalian subject by injection or implantation. The preferred embodiment of the preparation uses a polymerized albumin carrier containing immobilized glucose oxidase and peroxidase. The immobilized enzyme system is prepared in the form of small particles that can be administered either by implantation or by injection into tumour-bearing mammals. To date, antibacterial activity has been demonstrated only in vitro. Intraperitoneal injection into rats bearing Novikoff hepatomas or into mice bearing B16-Fl melanomas or L1210 (Lymphoid) tumours has resulted in the selected killing of tumour tissues. Several daily administrations are required to achieve complete destruction of the tumour. Additionally, the preparation is also active as a stimulant of the specific immune response to neoplasia. Its effect on the immune system could be useful in the stimulation of the immune system in diseases where the action of the endogenous immune system is insufficient.