公开(公告)号：EP0151536A3

公开(公告)日：1986-09-24

申请号：EP85300611

申请日：1985-01-30

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： Chambers, Donald A.

IPC分类号： C12Q01/52 ,  C12Q01/32 ,  G01N33/573 ,  G01N33/553

CPC分类号： C12Q1/52 ,  G01N33/553 ,  G01N33/573

摘要： An objective method for determining the presence of active periodontal disease in mammals, comprising assaying crevicular fluid for the presence of elevated levels of the enzyme aspartate aminotransferase. Also a kit whereby the presence of such disease can be determined.

公开(公告)号：EP0125867A3

公开(公告)日：1987-01-07

申请号：EP84303085

申请日：1984-05-08

申请人： Genetics International, Inc.

发明人： Davis, Graham ,  Hill, Hugh Allen Oliver ,  Cass, Anthony Edward George

IPC分类号： C12Q01/00 ,  C12Q01/32 ,  C12Q01/54

CPC分类号： A61B5/1486 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  Y10S435/817

摘要： This specification discloses methods of detection or measurement of an enzyme or of its specific substrate, and sensors used in such methods. The present invention is con- cenred with a multi-enzyme system, and the specification discloses, as one aspect of the invention a method of assay in which an electrode poised at a suitable potential is contacted with a system comprising a first enzyme, a cofactor linked with said enzyme and a mediator compound which transfers charge to the electrode from the first enzyme when its electrical state is changed by reaction of cofactor material. The cofactor may be NAD, NADP (both collectively referred to herein as NAD (P) CAMP ATP GTP TTP CTP. The specification particularly illustrates a method of assay in which an electrode (1) poised at a suitable potential is contact with a system comprising a first enzyme El a nicotinamide adenine dinucleotide compound N linked with said enzyme EI and a mediator compound F which transfers charge to the electrode from the first enzyme when its electrical state is changed by a NAD(P) NAD(P)H reaction. The NAD compound may act as a "bridge" between the said enryme// mediator system and further NAD utilizing enzymes E2.

公开(公告)号：EP0105443A3

公开(公告)日：1984-06-13

申请号：EP83109587

申请日：1983-09-27

申请人： BOEHRINGER MANNHEIM GMBH

发明人： Elstner, Erich, Prof. Dr. rer. nat.

IPC分类号： C12Q01/00 ,  C12Q01/28 ,  C12Q01/32

CPC分类号： C12Q1/28 ,  C12Q1/26 ,  Y10S435/81

公开(公告)号：EP0037742A3

公开(公告)日：1982-06-02

申请号：EP81301538

申请日：1981-04-08

申请人： NYEGAARD & CO. A/S

发明人： Yamanaka, Manabu ,  Mashige, Fumiko ,  Matsumura, Yoshinori ,  Skalhegg, Bjorn Arne

IPC分类号： C12Q01/32 ,  G01N33/74

CPC分类号： C12Q1/32

摘要： A reagent for the estimation of hydroxysteroids in serum .comprising a hydroxysteroid dehydrogenase, a coenzyme therefor, diaphorase, a tetrazolium salt and an inhibitor of non-steroidal hydroxyacid dehydrogenases, the said reagent being substantially free from hydroxysteroids and other serum components as well as a blank reagent for use in conjunction therewith in which the hydroxysteroid dehydrogenase is omitted.

公开(公告)号：EP0226427A3

公开(公告)日：1988-03-23

申请号：EP86309548

申请日：1986-12-08

申请人： SANWA KAGAKU KENKYUSHO CO., LTD.

发明人： Asai, Hiromoto ,  Asai, Masaru ,  Ito, Toshio ,  Fujii, Tokio ,  Furukawa, Eiji

IPC分类号： C12Q01/32 ,  G01N33/04

CPC分类号： C12Q1/32 ,  G01N33/04

摘要： A method for simultaneously inspecting quality of an animal milk and health of the animal secreting the milk and an indicator therefor, which method determines an amount of β-hydroxybutyric acid included in milk as a specimen by dropping it on the indicator comprising a mixture of a β-hydroxybutyric acid dehydrogenase, NAD, an electron carrier and a tetrazolium salt and comparing a color tone developed in the indicator with those in a predetermined standard color tone table.

公开(公告)号：EP0154409A3

公开(公告)日：1988-03-02

申请号：EP85300748

申请日：1985-02-05

申请人： UNITIKA LTD.

发明人： Watanabe, Mitsuo ,  Suzuki, Tadao ,  Kageyama, Masao

IPC分类号： C12Q01/32

CPC分类号： C12N9/0006 ,  C12Q1/32

摘要： A testing material for detecting alcohols is described, comprising (a) NAD or NADP, (b) alcohol dehydrogenase, (c) (1) thermophilic microorganism-derived diaphorase, or (2) diaphorase and dextran, (d) a dye and (e) a carrier. This testing material has very good storage stability and can measure alcohol concentrations, in particular ethanol concentrations in saliva, enzymatically with ease. It is useful in detecting and preventing driving while under the influence of liquor.

公开(公告)号：EP0135092A3

公开(公告)日：1987-04-22

申请号：EP84109133

申请日：1984-08-01

申请人： Yamasa Shoyu Kabushiki Kaisha ,  RIKAGAKU KENKYUSHO

发明人： Murachi, Takashi ,  Tabata, Masayoshi

IPC分类号： C12Q01/58 ,  C12N09/06 ,  C12Q01/32

CPC分类号： C12Q1/26 ,  C12Q1/32

摘要： The quantity of ammonia in a sample to be measured for ammonia content can be determined by:
(A) contacting the sample with L-glutamate dehydrogenase in the presence of reduced nicotinamide-adenine dinucleotide or reduced nicotinamide-adenine dinucleotide phosphate and a-ketoglutarate to produce glutamate in a quantity corresponding to the ammonia content in the sample; (B) catalyzing the resultant mixture by L-glutamate oxidase in the presence of oxygen to produce hydrogen peroxide in a quantity corresponding to the glutamate content in the resultant mixture; (C) measuring the quantity of hydrogen peroxide produced or the production rate thereof; and (D) calculating the ammonia content from the quantity of hydrogen peroxide produced or the production rate thereof thus measured. In accordance with the method of the present invention described above, it has become possible to determine the quantity of ammonia with high sensitivity in a short period of time by using as a signal a measurable value associated with hydrogen peroxide via a continuous autoanalyzing system.

公开(公告)号：EP0120440A3

公开(公告)日：1986-12-03

申请号：EP84103042

申请日：1984-03-20

申请人： BOEHRINGER MANNHEIM GMBH

发明人： Ameyama, Minoru, Prof. ,  Adachi, Osao, Prof.

IPC分类号： C12N09/04 ,  C12Q01/32

CPC分类号： C12N9/0006 ,  C12Q1/32 ,  C12Q1/61 ,  Y10S435/822 ,  Y10S435/823

公开(公告)号：EP0009222A3

公开(公告)日：1981-02-11

申请号：EP79103451

申请日：1979-09-17

申请人： American Monitor Corp.

发明人： Denney, Jerry W.

IPC分类号： C12Q01/00 ,  C12Q01/32 ,  C12Q01/50 ,  C12N09/96 ,  C12Q01/52 ,  C12Q01/54 ,  C12Q01/58

CPC分类号： C12Q1/58 ,  C12N9/96 ,  C12Q1/008 ,  C12Q1/52 ,  C12Q1/54

摘要： Prolonged stabilization (at least one month) of working reagent solutions of NADH (or NADPH) and of enzyme solutions, including both those used in measurement of enzymes and those in which enzymes are included for measurement of a non-enzyme substance, and stabilization of combinations thereof in certain assay systems.

摘要翻译： 包括NADH（或NADPH）和酶溶液的工作试剂溶液（包括用于测量酶的酶）和用于测量非酶物质的酶的酶稳定化（至少一个月）的稳定化（至少一个月） 在某些测定系统中的组合。

公开(公告)号：EP0166505A3

公开(公告)日：1988-09-21

申请号：EP85302252

申请日：1985-04-01

申请人： AKZO N.V.

发明人： Lattime, Hollis C.

IPC分类号： C12Q01/42 ,  C12Q01/32 ,  C12N09/16

CPC分类号： C12Q1/42 ,  C12N9/16 ,  C12Q2326/92

摘要： There is disclosed a process for isolating and quantitating an alkaline phosphatase comprising the steps of:
(1) separating the phosphatase from other enzymes in a biological sample; (2) reacting the phosphatase from step (1) with a co-enzyme precursor to produce a co-enzyme; (3) employing the co-enzyme produced in step (2) in a series of chemical reactions at least one of which uses a chromophore generation catalyst, to yield a chromophore, and (4) measuring the absorbance of the chromophore which is generated in step (3).

摘要翻译： 公开了一种分离和定量碱性磷酸酶的方法，包括以下步骤：（1）将生物样品中的磷酸酶与其他酶分离; （2）使来自步骤（1）的磷酸酶与辅酶前体反应以产生辅酶; （3）使用步骤（2）中产生的辅酶进行一系列化学反应，其中至少一种使用发色团产生催化剂产生发色团，和（4）测量生色团中产生的发色团的吸光度 步骤（3）。