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    METHODS AND COMPOSITIONS FOR MODIFYING GENOMIC DNA
    3.
    发明公开
    METHODS AND COMPOSITIONS FOR MODIFYING GENOMIC DNA 审中-公开
    VERFAHREN UND ZUSAMMENSETZUNGEN ZUR MODIFIZIERUNG VON GENOMISCHER DNA

    公开(公告)号：EP3132025A1

    公开(公告)日：2017-02-22

    申请号：EP15780038.4

    申请日：2015-04-13

    申请人： Maxcyte, Inc.

    发明人： LI, Linhong ,  PESHWA, Madhusudan

    IPC分类号： C12N5/10

    CPC分类号： C12N15/102 ,  A61K48/00 ,  C12N15/907 ,  C12N2800/80

    摘要： Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: transfecting the cells by electroporation with a composition comprising (a) a DNA oligo and (b) a DNA digesting agent wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.

    摘要翻译： 组合物和方法涉及内源基因组DNA区域的序列修饰。 某些方面涉及用于细胞中靶基因组DNA区域的位点特异性序列修饰的方法，包括：通过电穿孔转染细胞，所述组合物包含（a）DNA寡核苷酸和（b）DNA消化剂，其中供体DNA包含 （i）包含与靶基因组DNA区域同源的核酸序列的同源区和（ii）序列修饰区; 并且其中所述基因组DNA序列在靶基因组DNA区域特异性地被修饰。

    METHODS AND COMPOSITIONS FOR GENERATING STABLE TRANSFECTED CELLS
    7.
    发明公开
    METHODS AND COMPOSITIONS FOR GENERATING STABLE TRANSFECTED CELLS 审中-公开
    生产稳定转染细胞的方法和组合物

    公开(公告)号：EP2970915A4

    公开(公告)日：2016-10-26

    申请号：EP14763369

    申请日：2014-03-14

    申请人： MAXCYTE INC

    发明人： WANG WEILI ,  BRADY JAMES P ,  PESHWA MADHUSUDAN V

    IPC分类号： C12N5/10

    CPC分类号： C12N15/87 ,  C12N9/1205 ,  C12N2510/02 ,  C12Y207/01095

    摘要： Methods and compositions are provided involving high producing cell lines. Embodiments concern efficient methods for screening for such cell lines and for creating such cell lines. These cell lines can be used to create large amounts of protein. To quickly generate large quantity of recombinant proteins or vaccines for both pre-clinical study and clinical trials, almost all drug development will face the same challenging obstacle of rapidly generating a high stable producer. Developing and identifying a stable cell line is a critical part of biopharmaceutical development.

    METHODS AND DEVICES RELATED TO A REGULATED FLOW ELECTROPORATION CHAMBER
    8.
    发明公开
    METHODS AND DEVICES RELATED TO A REGULATED FLOW ELECTROPORATION CHAMBER 有权
    与调节流动电镀室相关的方法和装置

    公开(公告)号：EP1766057A2

    公开(公告)日：2007-03-28

    申请号：EP05748359.6

    申请日：2005-05-12

    申请人： Maxcyte, Inc.

    发明人： DZEKUNOV, Sergey ,  CHOPAS, Nicholas

    IPC分类号： C12Q1/68

    CPC分类号： C12M35/02 ,  B01L3/502 ,  B01L3/5027 ,  C12M41/00

    摘要： The electroporation chamber and its related devices combine the features of an electroporation chamber that acts as a manifold for regulation of sample flow with those of a flow electroporation device to form a regulated flow electroporation device. The invention further comprises a novel regulated flow electroporation chamber that enables conditions in which a sample is uniformly processed in individual fractions or volumes in a fully closed (sterile) system.

    摘要翻译： 电穿孔室及其相关装置将充当用于调节样品流动的歧管的电穿孔室的特征与流式电穿孔装置的特征组合以形成调节流式电穿孔装置。 本发明进一步包括新的调节流动电穿孔室，其能够实现样品在全封闭（无菌）系统中的单个级分或体积中均匀加工的条件。