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    THE USE OF BACTERIAL PHAGE ASSOCIATED LYTIC ENZYMES TO PREVENT FOOD POISONING
    1.
    发明公开
    THE USE OF BACTERIAL PHAGE ASSOCIATED LYTIC ENZYMES TO PREVENT FOOD POISONING 审中-公开
    BACTERIELLEN相关噬菌体来裂解酶防止食品中毒USE

    公开(公告)号：EP1333854A4

    公开(公告)日：2005-10-05

    申请号：EP01274135

    申请日：2001-11-01

    申请人： NEW HORIZONS DIAGNOSTICS CORP ,  UNIV ROCKEFELLER

    发明人： FISCHETTI VINCENT ,  LOOMIS LAWRENCE ,  TRUDIL DAVID

    IPC分类号： A23B4/22 ,  A23B5/16 ,  A23B7/155 ,  A23K1/165 ,  A23K1/17 ,  A23K3/00 ,  A23L3/3463 ,  A23L3/3571 ,  A61K38/43 ,  C12N9/36 ,  C12S3/00

    CPC分类号： A23B7/155 ,  A23B4/22 ,  A23B5/16 ,  A23K20/189 ,  A23K30/00 ,  A23L3/3463 ,  A23L3/3571

    摘要： The present invention discloses a method and composition for the treatment of bacterial contamination of food by the use of a phage associated lysing enzyme, preferably blended with an appropriate carrier. The method for treating food stuffs comprises treating the food stuffs with an anti-infection agent comprising an effective amount of at least one lytic enzyme produced by a bacteria infected with a bacteriophage specific for the bacteria.. Additionally, chimeric lytic enzymes shuffled lytic enzymes, and holin proteins, either alone or in combination, may be used to treat or prevent bacterial contamination of foodstuffs. The lytic enzyme can be used for the treatment or prevention of various strains of Staphylococcus, Streptococcus, Listeria, Salmonella, E. coli, Campylobacter, Pseudomonas, Brucella, other bacteria, and an,Y combination thereof. Feed for livestock, poultry and beef in slaughterhouses, canned and bottled goods, salad bars, and eggs are just some of the food items that can be treated with at least one lytic enzyme to reduce the risk of food contamination by bacteria.

    摘要翻译： 本发明盘通过使用相关联的裂解酶，优选与适当的载体混合的噬菌体的松用于食品的细菌污染的处理的方法和组合物。 用于治疗食品，该方法包括在抗感染剂治疗的食品有效量的由感染了噬菌体特异于细菌的细菌产生的至少一种溶解酶的包括。 的溶解酶是优选在pH为哪个溶解酶。所述的活性允许一个环境。 的溶解酶可用于治疗或预防金黄色葡萄球菌，链球菌属，利斯特氏菌属，沙门氏菌，大肠杆菌，弯曲杆菌属，假单胞菌属，布鲁氏杆菌的各种菌株，其它细菌和它们的任意组合。 家畜饲料，家禽屠宰场的牛肉，罐装和瓶装品，沙拉吧，和鸡蛋只是一些食品中也可以与至少一种裂解酶进行处理，以减少食品污染的细菌的风险。

    METHOD FOR DETECTION OF CONTAMINANTS
    2.
    发明公开
    METHOD FOR DETECTION OF CONTAMINANTS 失效
    检测方法的污染物的

    公开(公告)号：EP0901631A4

    公开(公告)日：2002-10-30

    申请号：EP97916812

    申请日：1997-03-25

    申请人： NEW HORIZONS DIAGNOSTICS CORP

    发明人： MILLER C DAVID ,  LOOMIS LAWRENCE

    IPC分类号： G01N33/48 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/06 ,  G01N1/02 ,  G01N33/543 ,  G01N33/569

    CPC分类号： C12Q1/04 ,  C12Q1/06 ,  C12Q2304/60 ,  G01N33/54366 ,  G01N33/56911 ,  G01N2001/028 ,  Y10S435/968

    摘要： A method and device for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species is described. The method consists of a means of collection of the microbes from an air, liquid, surface or other sample and suspending them in fluid phase (3). An aliquot of the fluid phase (3) is introduced into a disposable test device which allows filtration of the sample to concentrate the microbes and to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a somatic and bacterial releasing reagent to a disposable test device (25) which comprises a membrane (26) containing the luminescent reagents luciferin and luciferase, and introducing the disposable test device (25) into a luminometer that can read the luminescence from the underside.