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    MULTIDIMENSIONAL LIQUID CHROMATOGRAPHY SEPARATION SYSTEM AND SEPARATION METHOD FOR PROTEIN SEPARATION
    4.
    发明公开
    MULTIDIMENSIONAL LIQUID CHROMATOGRAPHY SEPARATION SYSTEM AND SEPARATION METHOD FOR PROTEIN SEPARATION 审中-公开
    MEHRDIMENSIONALESFLÜSSIGKEITSCHROMATOGRAFISCHESTRENNSYSTEM UND TRENNVERFAHREN ZUR PROTEINTRENNUNG

    公开(公告)号：EP2891881A1

    公开(公告)日：2015-07-08

    申请号：EP12883934.7

    申请日：2012-08-31

    申请人： Xi'an Aolan Science and Technology Co. ,  Northwest University

    发明人： GENG, Xindu

    IPC分类号： G01N30/02 ,  G01N30/10 ,  G01N30/20

    CPC分类号： B01D15/1878 ,  B01D15/30 ,  B01D15/34 ,  B01D15/362 ,  B01D15/424 ,  C07K1/16 ,  G01N30/10 ,  G01N30/20 ,  G01N30/462 ,  G01N30/463 ,  G01N30/465 ,  G01N2030/8831

    摘要： A multidimensional liquid chromatography separation system and separation method for protein separation. The multidimensional liquid chromatography separation system for protein separation comprises a mobile-phase storage tank (2), a first liquid transfer device (3, 1-2), a second liquid transfer device (9, 1-1), a first sample introduction device (4-1, 4-2), a second sample introduction device (12), a separation device (7), a collection and storage device (10), at least two drainage devices (7-1, 7-2, 7-3) and a flow path switching device (11). When the liquid chromatography separation of each dimension is conducted, the collection and storage device collects target middle distillates needing to conduct the next step of liquid chromatography separation and stores same in a collection and storage device. During the liquid chromatography separation of the second dimension or more dimensions, all the target middle distillates from the collection and storage device are mixed with mobile phases through a sample introduction mixer of the second sample introduction device, and the amount of the mobile phases conveyed by same is adjusted and measured using the first liquid transfer device and the second liquid transfer device, so as to enable the eluent concentration in the sample introduction mixture to be lower than the critical migration eluent concentration of all the target proteins in the target middle distillates which need to be preserved in the separation of this dimension.

    摘要翻译： 一种多维液相色谱分离系统和分离蛋白质分离方法。 用于蛋白质分离的多维液相色谱分离系统包括流动相储存罐（2），第一液体输送装置（3,2-1），第二液体转移装置（9-1-1），第一样品引入 装置（4-1,4-2），第二样品引入装置（12），分离装置（7），收集和存储装置（10），至少两个排水装置（7-1,7-2， 7-3）和流路切换装置（11）。 当进行每个维度的液相色谱分离时，收集和收集装置收集需要进行下一步液相色谱分离的目标中间馏分，并将其存储在收集和储存装置中。 在第二维或更多维的液相色谱分离期间，来自收集和储存装置的所有目标中间馏分通过第二样品引入装置的样品引入混合器和流动相的量与流动相混合，并且通过 使用第一液体转移装置和第二液体转移装置进行相同的调整和测量，以使样品导入混合物中的洗脱液浓度低于目标中间馏分中所有目标蛋白质的临界迁移洗脱液浓度， 需要保留在这个维度的分离。

    A CAKY CHROMATOGRAPHIC COLUMN AND THE METHOD FOR PRODUCING IT AND ITS APPLICATIONS
    8.
    发明授权
    A CAKY CHROMATOGRAPHIC COLUMN AND THE METHOD FOR PRODUCING IT AND ITS APPLICATIONS 有权
    蛋糕形色谱法用于生产和及其应用

    公开(公告)号：EP1396721B1

    公开(公告)日：2008-08-06

    申请号：EP02732319.5

    申请日：2002-05-16

    申请人： Northwest University

    发明人： GENG, Xindu Institute Of Modern Separation Science ,  ZHANG, Y., Institute Of Modern Separation Science

    IPC分类号： G01N30/60 ,  G01N30/56 ,  B01D15/08

    CPC分类号： G01N30/56 ,  B01D15/206 ,  B01D15/22 ,  G01N30/606 ,  G01N2030/562

    摘要： The present invention discloses a caky chromatographic column and the method for producing it and its application. The caky chromatographic column includes a chromatographic medium column with an inlet and an exit for mobile phase and chromatographic medium packed in the inner cavity of the chromatographic medium column. The rate of thickness to radial length of the said inner cavity of the chromatographic medium is less than or equal to 1. The method for producing the caky chromatographic column comprises the steps of: 1) producing a chromatographic medium column that includes upper and lower pressing plates combined together with the inlet and exit for the mobile phase and a column body with at least one side-hole, the rate of the thickness to the radial length of the inner cavity of the chromatographic medium is less than or equal to 1; 2) injecting the chromatographic medium into the inner cavity of the chromatographic medium column through the side-hole of the chromatographic column. The caky chromatographic column can be used for the separation and purification of the biomacromolecules.

    A CAKY CHROMATOGRAPHIC COLUMN AND THE METHOD FOR PRODUCING IT AND ITS APPLICATIONS
    9.
    发明公开
    A CAKY CHROMATOGRAPHIC COLUMN AND THE METHOD FOR PRODUCING IT AND ITS APPLICATIONS 有权
    KUCHENFÖRMIGECHROMATOGRAPHIESÄULE，VERFAHREN ZU IHRER HERSTELLUNG UND IHRE ANWENDUNGEN

    公开(公告)号：EP1396721A1

    公开(公告)日：2004-03-10

    申请号：EP02732319.5

    申请日：2002-05-16

    申请人： Northwest University

    发明人： GENG, Xindu Institute Of Modern Separation Science ,  ZHANG, Y., Institute Of Modern Separation Science

    IPC分类号： G01N30/60 ,  G01N30/56 ,  B01D15/08

    CPC分类号： G01N30/56 ,  B01D15/206 ,  B01D15/22 ,  G01N30/606 ,  G01N2030/562

    摘要： The present invention discloses a kind of chromatographic cake and its manufacturing method and application. The aim of this invention is to offer a kind of chromatographic cake and its manufacturing method applicable to the separation and simultaneous renaturation and purification of biopolymers with excellent effect. Said chromatographic cake includes a chromatographic packing cake with the mobile phase inlet and outlet and a chromatographic packing packed in the inner cavity of the chromatographic packing cake. The ratio of thickness and the radial length of the inner cavity of the chromatographic packing cake are smaller than or equal to 1. A method of manufacturing chromatographic cakes comprises the steps of 1) Manufacturing the chromatographic packing cake, the chromatographic packing cake includes the combined upper and lower clamp plates separately with the said mobile phase inlet and outlet and a cake body with at least one lateral hole; the ratio of the thickness to the radial length of the inner cavity of the chromatographic packing cake is smaller than, or equal to 1; and 2) adding chromatographic packing from the lateral hole of the chromatographic packing cake in the inner cavity of the chromatographic-packing cake.

    摘要翻译： 本发明公开了一种色谱饼及其制造方法和应用。 本发明的目的是提供一种色谱饼及其制造方法，适用于生物聚合物的分离和同时复性和纯化，效果极佳。 所述色谱图包括带有流动相进样口和出口的色谱填充饼，并将色谱填料包装在色谱填充饼的内腔中。 色谱填充饼的内腔的厚度和径向长度的比例小于或等于1.制备色谱饼的方法包括以下步骤：1）制备色谱填充饼，色谱填充饼包括组合 上下夹板分别与所述流动相入口和出口以及具有至少一个侧孔的滤饼体; 色谱填料饼的内腔厚度与径向长度之比小于或等于1; 和2）从色谱填充饼的内腔中的色谱填充饼的侧孔加入色谱填料。