公开(公告)号：EP2632507B1

公开(公告)日：2014-09-24

申请号：EP11771037.6

申请日：2011-10-18

申请人： Fresenius Medical Care Deutschland GmbH

发明人： LEINENBACH, Hans-Peter ,  GERNER, Franz-Josef ,  KUHN, Stefan ,  SCHINZEL, Ekkehard ,  PRIESNITZ, Patrick

IPC分类号： A61M1/36 ,  B01D15/20 ,  G01N30/56

CPC分类号： A61M1/36 ,  A61M1/3679 ,  B01D15/206 ,  B01D15/22 ,  G01N30/56 ,  G01N30/606 ,  G01N2030/562 ,  Y10T156/1062

公开(公告)号：EP2324347A1

公开(公告)日：2011-05-25

申请号：EP09788310.2

申请日：2009-09-09

申请人： De Staat Der Nederlanden, Vert. Door De Minister Van VWS

发明人： VAN ELS, Cécile, Antoinette, Carola, Maria ,  SOETHOUT, Ernst, Christiaan ,  DE JONG, Adrianus, Petrus, Josephus, Maria ,  MEIRING, Hugo, Derk

IPC分类号： G01N30/56 ,  G01N30/60 ,  G01N30/72 ,  G01N30/46 ,  C07K16/00 ,  F16L19/00 ,  H01J49/16

CPC分类号： G01N30/7266 ,  G01N30/463 ,  G01N30/56 ,  G01N30/6039 ,  G01N2030/562 ,  G01N2030/565 ,  H01J49/167

摘要： The present invention relates to an improved LCMS technology and its uses in methods for the selective identification and characterization of immunogenic pathogen associated epitopes, and the use thereof in vaccine development. One way of bridging the knowledge gap on T cell epitopes is to apply a new platform technology, ''immunoproteomics'', to directly assess the epitope display at the surface of antigen presenting cells by nanoscale mass spectrometry of extracted peptide samples. This is the only methodology that can provide unbiased insight into epitope features such as the exact molecular nature, diversity, abundance, dynamics and PTM of T cell epitopes originating from pathogen-derived proteins. Therefore, this platform technology and immunoproteomics should become an intrinsic part of vaccinology.

摘要翻译： 本发明涉及一种改进的LCMS技术及其在免疫原性病原体相关表位的选择性鉴定和鉴定方法中的应用及其在疫苗开发中的应用。 桥接T细胞表位知识差距的一种方法是应用新的平台技术“免疫蛋白组学”，以通过提取的肽样品的纳米质谱直接评估抗原呈递细胞表面的表位显示。 这是唯一可以提供无偏见的表位特征的方法，例如源自病原体衍生蛋白的T细胞表位的确切分子性质，多样性，丰度，动力学和PTM。 因此，这种平台技术和免疫蛋白组学应该成为疫苗学的内在组成部分。

公开(公告)号：EP1355709A4

公开(公告)日：2008-12-24

申请号：EP01989251

申请日：2001-12-27

申请人： COHESIVE TECH INC

发明人： BRANN JOHN

IPC分类号： G01N30/24 ,  G01N30/46 ,  B01D15/08 ,  B01J20/281 ,  G01N30/26 ,  G01N30/32 ,  G01N30/62 ,  G01N30/72 ,  G01N30/88

CPC分类号： G01N30/466 ,  B01D15/18 ,  B01D15/1864 ,  G01N30/24 ,  G01N30/7233 ,  G01N30/7266 ,  G01N30/88 ,  G01N2030/562 ,  G01N2030/567 ,  G01N2030/628 ,  G01N2030/8804

摘要： The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a plurality of chromatographic systems, each system comprising one or more pumps and one or more chromatography columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

公开(公告)号：EP1725863A2

公开(公告)日：2006-11-29

申请号：EP05733903.8

申请日：2005-02-14

申请人： Bio-Rad Pasteur

发明人： VIDALINC, Pierre

IPC分类号： G01N30/56

CPC分类号： B01D15/206 ,  G01N30/56 ,  G01N30/6052 ,  G01N2030/204 ,  G01N2030/562

摘要： A module (1) for dry loading and unloading of a chromatography resin, a chromatography column (3) and a method for using such a module (1). The column (3) comprises an inlet valve (39) adapted to load dry chromatography resin particles and an outlet port (35) for pumping the air from de column (3). The outlet port (35) is located above the inlet valve (39).

公开(公告)号：EP1485328A1

公开(公告)日：2004-12-15

申请号：EP03716653.5

申请日：2003-03-18

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： TAI, Yu-Chong ,  HE, Qing

IPC分类号： C03C1/00

CPC分类号： B81C1/00333 ,  B01L3/502707 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0896 ,  B81C1/00182 ,  B81C1/00476 ,  B81C2201/0108 ,  B82Y30/00 ,  G01N30/6095 ,  G01N2030/562 ,  G01N2030/6056

摘要： A method for integrally fabricated MEMs devices with include micro or nanoparticles by providing a mixture (16) of sacrificial material (12) and a multiplicity of particles (10), disposing the mixture (16) on a substrate (14), fabricating a MEMs structure on the substrate (14) including at least part of the mixture (16), so that at least some of the mixture (16) is enclosed in the MEMs structure, removing the sacrificial material (12), and leaving at least some of the multiplicity of particles (10) substantially free and enclosed in the MEMs structure. The step of fabricating a MEMs structure is quite general and is contemplated as including one or a multiplicity of additional steps for creating some type of structure in which the particles (10), which may be microbeads or nanobeads, are included.

公开(公告)号：EP1126275A3

公开(公告)日：2002-12-18

申请号：EP01301043.4

申请日：2001-02-06

申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

发明人： Zare, Richard N. ,  Dulay, Maria T. ,  Chen, Jing-Ran

IPC分类号： G01N27/447

CPC分类号： G01N27/44704 ,  G01N27/44747 ,  G01N30/6004 ,  G01N30/603 ,  G01N2030/285 ,  G01N2030/562

摘要： A separation column is provided with a photopolymer component which, when irradiated, causes controlled porosity polymerization. A particularly preferred embodiment is wherein the separation medium is retained by a photopolymer frit, which can be reliably and reproducibly generated with controlled porosity.

公开(公告)号：EP1085930A1

公开(公告)日：2001-03-28

申请号：EP99927058.0

申请日：1999-05-12

申请人： TRIKONEX AB

发明人： FEX, Tomas

IPC分类号： B01D15/08 ,  G01N30/60

CPC分类号： B01D15/18 ,  B01D15/24 ,  B01J20/281 ,  B01J2219/00274 ,  B01J2220/54 ,  G01N30/02 ,  G01N30/48 ,  G01N30/6017 ,  G01N30/6082 ,  G01N2030/165 ,  G01N2030/524 ,  G01N2030/562 ,  B01D15/26 ,  G01N30/62 ,  G01N30/95 ,  G01N21/64

摘要： A chromatographic column for simultaneous purification of several compounds in a sample by dry column chromatography comprised by a plastic tube and containing a sorbent is described, wherein there is an empty volume on top of the sorbent large enough to hold the amount of developing solvent needed to develop the column, wherein the plastic material is transparent or semitransparent to UV and visible light and can be cut with a sharp object, and wherein the sorbent particle size is

公开(公告)号：EP0255049A2

公开(公告)日：1988-02-03

申请号：EP87110684.5

申请日：1987-07-23

申请人： Tosoh Corporation

发明人： Sakamoto, Kiyoaki ,  Nakahara, Yasuhiro

IPC分类号： B01D15/08

CPC分类号： B01D15/08 ,  G01N30/56 ,  G01N30/6004 ,  G01N2030/027 ,  G01N2030/562

摘要： The high density filler type filtration equipment comprises a cylindrical column body (1) with an end flange (1a) near the upper and lower end. At the root of both ends of the end flange (1a), a circumferential groove (6b) is formed, where the end of a seal ring (6) is to be lockably inserted. A guide face (1c) is formed as the outer circumferential side face of the predetermined length in the direction of both sides of the end. A cover (2) which is lockably fitted to the end of the column body and on the circumferential edge of which there is provided a bolt hole (2b) which is opposite to the bolt hole (1b) of the end flange (1a). The process liquid flows in a fluid path (2a). Furthermore, there are provi ded an inner dent (2c) of the cover with a seal ring (6), stainless steel multi-hole plate (7) as a partition membrane, and a ring gasket (8). On the circumferential wall of the inner dent (2c) is a circumferential groove (2d) which acts as the fixture of the seal ring (6) when the outer circumferential flange is mounted and as a stopper for a stainless multi-hole plate (7). A tightening bolt (3) with a nut tightly fixes the column body (1) and the cover (2) with the seal ring (6) in between. This filtration equipment provides for a high density and uniform filling of the filler and can be easily assembled.

摘要翻译： 高密度填料型过滤设备包括具有靠近上端和下端的端部凸缘（1a）的圆柱形柱体（1）。 在端部凸缘（1a）的两端的根部处形成有密封环（6）的端部可被锁定插入的周向槽（6b）。 引导面（1c）形成为在端部的两侧方向上的预定长度的外周侧面。 一个盖子（2），其可锁定地装配到柱体的端部，并且在其周缘上设置有与端部凸缘（1a）的螺栓孔（1b）相对的螺栓孔（2b）。 处理液体在流体路径（2a）中流动。 此外，还提供了具有密封环（6），作为分隔膜的不锈钢多孔板（7）和环形垫圈（8）的盖的内凹部（2c）。 内凹部（2c）的周壁上形成作为外周凸缘安装时的密封环（6）的固定件的周向槽（2d），作为不锈钢多孔板（7 ）。 具有螺母的紧固螺栓（3）将柱体（1）和盖（2）与密封环（6）紧密地固定在其间。 该过滤设备提供了填料的高密度和均匀填充，并且可以容易地组装。

公开(公告)号：EP0248379A2

公开(公告)日：1987-12-09

申请号：EP87107891.1

申请日：1987-06-01

申请人： Magyar Tudományos Akadémia Kutatási Eszközöket Kivitelezö Vállalata

发明人： Poppe, László, Dipl.-Ing. ,  Meisel, Tibor, Dipl.-Ing. ,  Agai, Béla, Adjunkt ,  Novák, Lajos

IPC分类号： G01N30/60 ,  G01N30/56 ,  B01D15/08

CPC分类号： B01D15/125 ,  B01D15/206 ,  B01D15/22 ,  G01N30/6004 ,  G01N30/6026 ,  G01N30/6065 ,  G01N2030/562

摘要： Die erfindungsgemässe nachfüllbare Niederdruckkolonne für präparative Chromatographie weist einen Ab­sorptionslösung enthaltenden rohrförmigen Kolonnen­körper /1/ auf, an dessen beiden Enden je ein Hals­abschnitt /2/ ausgebildet ist und in jedem Halsab­schnitt /2/ ein gegen axiale Bewegung befesitigter und einen Einströmkanal /3/ enthaltender Kern /4/ ange­ordnet ist, zwischen dem und der Wandung des Halsab­schnittes /2/ mindestens ein elastischer Dichtring /7/ axial unverschiebbar angeordnet ist, und dem mittels einem an Halsabschnitt /2/ des Kolonnenkörpers /1/ lösbar befestigten Element /9/ in seiner Lage fest­gehaltenen Kern /4/ ist ein Filter- und Verteiler­element /5/ unmittelbar, im Weg des Einströmkanals /3/ angeschlossen.

摘要翻译： 根据本发明的柱具有包含吸收溶液的管状体（1），并且其两端在每种情况下形成一个颈部（2）。 在每个颈部（2）中设置的是芯部（4），其被抵抗轴向运动固定并且包含入口通道（3），颈部部分（2）的壁与至少一个弹性密封环（7）之间， 以轴向不可移动的方式设置。 过滤器和分配器元件（5）通过可拆卸地安装在颈部（2）上的元件（9）直接连接在入口通道（3）的路径到芯部（4） 的柱体（1）。

公开(公告)号：EP2362215B1

公开(公告)日：2017-11-22

申请号：EP11163657.7

申请日：2001-12-27

申请人： Cohesive Technologies Inc.

发明人： Brann, John

IPC分类号： G01N30/46 ,  G01N30/24 ,  B01D15/08

CPC分类号： G01N30/466 ,  B01D15/18 ,  B01D15/1864 ,  G01N30/24 ,  G01N30/7233 ,  G01N30/7266 ,  G01N30/88 ,  G01N2030/562 ,  G01N2030/567 ,  G01N2030/628 ,  G01N2030/8804

摘要： The present invention relates to an apparatus and method for chromatographically analyzing each of a plurality of samples comprising: (a) an autosampler to contain a plurality of samples for chromatographic analysis; (b) a plurality of chromatographic systems, each system comprising a chromatography column and at least one pump associated with the column to establish a flow of eluant from each column following a selected elution protocol; (c) a single detector for analyzing the eluant from each of said chromatographic systems; (d) a valve positioned between said detector and said chromatographic systems, said valve selectively permitting flow of eluant from each chromatography system to reach the detector in sequence; and (e) a computer control device that considers the samples and as input information an anticipated data collecting window for each sample at the detector, the computer control device selecting samples for introduction to said plurality of chromatographic systems and controlling the valve to selectively direct eluant flow from said plurality of chromatographic systems to the detector, where the selecting of samples and the controlling of the valve is based on the input information of the samples such that each sample is sequentially analyzed during its data collection window by the detector.