公开(公告)号：EP0883810A1

公开(公告)日：1998-12-16

申请号：EP97907618.0

申请日：1997-02-13

申请人： PARACELSIAN, INC.

发明人： WHEELOCK, Geoffrey, D.

IPC分类号： G01N33

CPC分类号： G01N33/567 ,  G01N33/5308 ,  G01N2333/705 ,  G01N2430/40 ,  Y10S436/815

摘要： A method of detecting dioxin-like compounds consisting essentially of the group of compounds: polychlorinated dibenzodioxins, polychlorinated dibenzofurans, polychlorinated biphenyls, and structural analogues thereof which exhibit biological activity characteristic of polychlorinated dibenzodioxins, polychlorinated dibenzofurans, polychlorinated biphenyls. The assay includes: 1) an inactive Ah receptor in a form capable of binding to the dioxin-like compounds and being tranformed to an active form that forms a complex with ARNT and binds a dioxin responsive element, and 2) a quantity of ARNT sufficient to optimize Ah receptor transformation. A test sample is contacted with the assay under conditions effective to bind the dioxin-like compounds to the Ah receptor and allow transformation of the Ah receptor to an active form that forms a complex with ARNT. The presence of the complex containing the transformed Ah receptor and the ARNT is detected. The presence of the complex containing the transformed Ah receptor and the ARNT is detected with an antibody with a region capable of binding to the ARNT when associated with the complex.

公开(公告)号：EP0680609A4

公开(公告)日：1998-07-08

申请号：EP94907896

申请日：1994-01-21

申请人： PARACELSIAN INC

发明人： BABISH JOHN G ,  MA XINFANG ,  RININGER JOSEPH A ,  JOHNSON BRIAN E ,  WHITING DEBRA S

IPC分类号： C12Q1/50 ,  G01N33/50 ,  G01N33/573 ,  G01N33/574 ,  G01N33/53

CPC分类号： C12Q1/50 ,  G01N33/5017 ,  G01N33/573 ,  G01N33/57496 ,  G01N2333/912

摘要： In vivo and (in vitro) assays are disclosed for measuring cyclin dependent kinase concentrations in cells or tissues, methods are provided for their use, including the use of such assays to evaluate carcinogenicity of a test compound, potential antineoplastic agents, and effectiveness of regions for increasing cell growth.

公开(公告)号：EP1105146A1

公开(公告)日：2001-06-13

申请号：EP99934036.7

申请日：1999-07-14

申请人： PARACELSIAN, INC.

发明人： LANDES, Bernard ,  WHEELOCK, Geoffrey ,  RINIGER, Joseph, A. ,  FRANCK, Zsofia

IPC分类号： A61K35/78

CPC分类号： G01N33/5058 ,  C12Q1/37 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5038 ,  G01N33/5044 ,  G01N33/505 ,  G01N33/5055 ,  G01N33/80 ,  G01N33/9406 ,  G01N2333/57 ,  G01N2500/00

摘要： The present invention provides methods for evaluating the biofunctionality of natural products. Since natural products typically comprise a variety of active components, both identified and unidentified, and normal dosage is by ingestion, methods of the invention simulate normal physiological digestion and absorption from the digestive tract. Methods are disclosed which provide for evaluation of biofunctionality and quality assurance testing for natural compositions such as herbs, herbal extracts and formulations derived therefrom, even though active components and their specific activities may remain unidentified.

公开(公告)号：EP0680609A1

公开(公告)日：1995-11-08

申请号：EP94907896.0

申请日：1994-01-21

申请人： PARACELSIAN, INC.

发明人： BABISH, John, G. ,  MA, Xinfang ,  RININGER, Joseph, A. ,  JOHNSON, Brian, E. ,  WHITING, Sebra, S.

IPC分类号： C12Q1 ,  G01N33

CPC分类号： C12Q1/50 ,  G01N33/5017 ,  G01N33/573 ,  G01N33/57496 ,  G01N2333/912

摘要： In vivo and (in vitro) assays are disclosed for measuring cyclin dependent kinase concentrations in cells or tissues, methods are provided for their use, including the use of such assays to evaluate carcinogenicity of a test compound, potential antineoplastic agents, and effectiveness of regions for increasing cell growth.