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    PRODUCTION OF THAPSIGARGINS BY THAPSIA CELL SUSPENSION CULTURE
    3.
    发明公开
    PRODUCTION OF THAPSIGARGINS BY THAPSIA CELL SUSPENSION CULTURE 审中-公开
    ThAPSIA细胞悬液培养生产THAPSIGARGINS

    公开(公告)号：EP3077523A1

    公开(公告)日：2016-10-12

    申请号：EP14835563.9

    申请日：2014-12-02

    申请人： Phyton Holdings, LLC

    发明人： GORR, Gilbert ,  HECKENMÜLLER, Harald ,  WILKE, Jens, Stefan ,  ULLISCH, David, Alexander ,  SANKAR-THOMAS, Yantree, Devi

    IPC分类号： C12P5/00 ,  C12P17/04 ,  C12N5/00

    摘要： The present invention relates to a method of producing sesquiterpene lactones of the thapsigargin family, the method comprising the steps of: (a) culturing plant cells of the genus Thapsia in a nutrient medium in a suspension cell culture, wherein the cells produce one or more sesquiterpene lactones of the thapsigargin family; and (b) recovering one or more sesquiterpene lactones of the thapsigargin family produced in (a). The present invention further relates to a suspension cell culture comprising plant cells of the genus Thapsia, wherein the plant cells are capable of producing one or more sesquiterpene lactones of the thapsigargin family and to a plant cell biomass comprising plant cells of the genus Thapsia obtained from said suspension cell culture.

    摘要翻译： 本发明涉及生产毒胡萝卜素家族的倍半萜内酯的方法，所述方法包括以下步骤：（a）在悬浮细胞培养物中的营养培养基中培养Thapsia属的植物细胞，其中所述细胞产生一种或多种 毒胡萝卜素家族的倍半萜内酯; 和（b）回收（a）中产生的一种或多种倍半萜内酯类毒胡萝卜素家族。 本发明进一步涉及含有Thapsia属植物细胞的悬浮细胞培养物，其中所述植物细胞能够产生毒胡萝卜素家族的一种或多种倍半萜内酯，并且涉及含有从Thapsia获得的植物细胞的植物细胞生物质， 所述悬浮细胞培养物。