公开(公告)号：EP4148123A1

公开(公告)日：2023-03-15

申请号：EP20934683.2

申请日：2020-05-26

申请人： Qingdao Restore Biotechnology Co., Ltd.

发明人： ZHANG, Bingqiang ,  CHEN, Mengmeng

IPC分类号： C12N5/0783 ,  C40B50/06 ,  A01N1/02

摘要： The present invention discloses an in-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells. The method includes the follows: using a dedicated amplification medium of immune cells to perform first-stage amplification culture on mononuclear cells to obtain preliminarily amplified immune cells; using a dedicated induction medium of immune cells to perform second-stage induction and amplification culture on the preliminarily amplified immune cells to obtain induced immune cells; using a dedicated activation medium of immune cells to perform third-stage activation and amplification culture on the induced immune cells to obtain a large number of immune cells with activation functions; using a dedicated cryopreserving fluid of immune cells to cryopreserve the immune cells to obtain cryopreserved immune cells; and performing preservation according to ABO/RH typing and HLA typing; and establishing an information file of immune cells for retrieval to construct an immune cell bank.

公开(公告)号：EP4141109A1

公开(公告)日：2023-03-01

申请号：EP20873344.4

申请日：2020-05-25

申请人： Qingdao Restore Biotechnology Co., Ltd.

发明人： ZHANG, Bingqiang ,  CHEN, Mengmeng ,  ZOU, Wei ,  FU, Xueqi

IPC分类号： C12N5/079 ,  C12N5/00

摘要： The present invention relates to the field of differentiation induction of stem cells, in particular to a serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell. The serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell is prepared by the following method: uniformly mixing the serum-free complete medium for differentiation induction, containing 5-10 µmol of resveratrol, 2-4 µmol of icariin, 1-3 nmol of aspirin, 1-3 nmol of parathyroid hormone, 5-10 nmol of hydrocortisone, 1-3 mg of rapamycin, 2-10 µg of testosterone, 2-10 µg of EPO, 2-10 µg of LIF and the balance of a corneal epithelial cell serum-free medium in per 1 L; and then performing sterilization by filtration. The serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell of the present invention uses traditional Chinese medicine components of resveratrol and icariin in combination with aspirin, parathyroid hormone, hydrocortisone, rapamycin, testosterone and growth factors to cooperatively induce the directional differentiation of a mesenchymal stem cell to a corneal epithelial cell, uses nontoxic induction components, is high in induction efficiency and short in induction time, and achieves high induced corneal epithelial cell activity, no cell transplantation rejection, no ethical problem and high safety.

公开(公告)号：EP4159842A1

公开(公告)日：2023-04-05

申请号：EP20937954.4

申请日：2020-06-01

申请人： Qingdao Restore Biotechnology Co., Ltd.

发明人： ZHANG, Bingqiang ,  CHEN, Mengmeng

IPC分类号： C12N5/0775 ,  A61K35/28 ,  C12N5/00

摘要： The present invention discloses a method for screening, activating, amplifying and cryopreserving mesenchymal stem cells in vitro and establishing a cell bank of mesenchymal stem cells. The method includes the following steps: using a dedicated primary screening medium of mesenchymal stem cells for first-stage screening culture to obtain purified mesenchymal stem cells; using a dedicated activation and amplification medium of mesenchymal stem cells to perform second-stage activation and large-scale amplification culture on the purified mesenchymal stem cells to obtain a large number of mesenchymal stem cells with activation functions; using a dedicated cryopreserving fluid of mesenchymal stem cells to cryopreserve the stem cells and performing preservation according to ABO/RH typing and HLA typing; and establishing an information file for retrieval to construct a mesenchymal stem cell bank.

公开(公告)号：EP4092106A1

公开(公告)日：2022-11-23

申请号：EP20838869.4

申请日：2020-05-25

申请人： Qingdao Restore Biotechnology Co., Ltd.

发明人： ZHANG, Bingqiang ,  CHEN, Mengmeng ,  LI, Cuicui ,  WANG, Erpu ,  WANG, Fubin ,  ZOU, Wei ,  FU, Xueqi ,  LIU, Cuijuan

IPC分类号： C12N5/071 ,  C12N5/0775

摘要： The present invention belongs to the field of biomedicine, and relates to an inducer for inducing a mesenchymal stem cell to differentiate into an islet cell. An inducer for inducing a mesenchymal stem cell to differentiate into an islet cell consisted of the following components: GLP-1, parathyroid hormone, paracetamol, rapamycin, icariin, trametinib, EPO and VEGF. Each component in a inducer for inducing a mesenchymal stem cell to differentiate into an islet cell of the present invention is safe and non-toxic, requiring fewer steps and short time to induce differentiation, with high induction efficiency.