公开(公告)号：EP4141109A1

公开(公告)日：2023-03-01

申请号：EP20873344.4

申请日：2020-05-25

申请人： Qingdao Restore Biotechnology Co., Ltd.

发明人： ZHANG, Bingqiang ,  CHEN, Mengmeng ,  ZOU, Wei ,  FU, Xueqi

IPC分类号： C12N5/079 ,  C12N5/00

摘要： The present invention relates to the field of differentiation induction of stem cells, in particular to a serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell. The serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell is prepared by the following method: uniformly mixing the serum-free complete medium for differentiation induction, containing 5-10 µmol of resveratrol, 2-4 µmol of icariin, 1-3 nmol of aspirin, 1-3 nmol of parathyroid hormone, 5-10 nmol of hydrocortisone, 1-3 mg of rapamycin, 2-10 µg of testosterone, 2-10 µg of EPO, 2-10 µg of LIF and the balance of a corneal epithelial cell serum-free medium in per 1 L; and then performing sterilization by filtration. The serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell of the present invention uses traditional Chinese medicine components of resveratrol and icariin in combination with aspirin, parathyroid hormone, hydrocortisone, rapamycin, testosterone and growth factors to cooperatively induce the directional differentiation of a mesenchymal stem cell to a corneal epithelial cell, uses nontoxic induction components, is high in induction efficiency and short in induction time, and achieves high induced corneal epithelial cell activity, no cell transplantation rejection, no ethical problem and high safety.

公开(公告)号：EP4092106A1

公开(公告)日：2022-11-23

申请号：EP20838869.4

申请日：2020-05-25

申请人： Qingdao Restore Biotechnology Co., Ltd.

发明人： ZHANG, Bingqiang ,  CHEN, Mengmeng ,  LI, Cuicui ,  WANG, Erpu ,  WANG, Fubin ,  ZOU, Wei ,  FU, Xueqi ,  LIU, Cuijuan

IPC分类号： C12N5/071 ,  C12N5/0775

摘要： The present invention belongs to the field of biomedicine, and relates to an inducer for inducing a mesenchymal stem cell to differentiate into an islet cell. An inducer for inducing a mesenchymal stem cell to differentiate into an islet cell consisted of the following components: GLP-1, parathyroid hormone, paracetamol, rapamycin, icariin, trametinib, EPO and VEGF. Each component in a inducer for inducing a mesenchymal stem cell to differentiate into an islet cell of the present invention is safe and non-toxic, requiring fewer steps and short time to induce differentiation, with high induction efficiency.