公开(公告)号：EP0842260A2

公开(公告)日：1998-05-20

申请号：EP96921676.0

申请日：1996-06-14

申请人： RCR Scientific, Inc.

发明人： ROTH, Jonathan, N. ,  BONTRAGER, Gordon

IPC分类号： C12M1 ,  B05C7 ,  B05C13

CPC分类号： B05C13/00 ,  B05C7/04 ,  C12M23/10

摘要： A plurality of petri dishes (44) or other glass or plastic containers are coated on the inside bottom surface of the containers by passing the containers through a coating or layering apparatus (18). The apparatus includes an indexing conveyor (12) to sequentially pass the open containers through a filling station where an amount of the coating material is applied while tilting the container to one side thereby distributing the material to the entire bottom surface of the container. The containers are conveyed to an aspirating station (20) to aspirate excess material while tilting the container toward the aspirating nozzle (122). Subsequent to the aspiration step, the containers are tilted in the opposite direction to distribute the material in the container. The material is then dried and the containers stacked and packaged. The coating material includes metal ions such as calcium or other divalent metal ions, in a suitable carrier that is able to bond the bottom inside surface of the container. The coating material forms a dehydrated, solidified layer bonded onto the bottom surface of the container. The coating material can be a water/agar solution or a plastic resin in an appropriate solvent containing the calcium ions.

公开(公告)号：EP0897428B2

公开(公告)日：2011-07-13

申请号：EP97915186.7

申请日：1997-03-21

申请人： RCR Scientific, Inc.

发明人： ROTH, Jonathan, N. ,  BONTRAGER, Gordon, L.

IPC分类号： C12Q1/04 ,  C12Q1/54 ,  C12Q1/18 ,  C12Q1/02 ,  C12Q1/22 ,  C12N5/00 ,  C12N1/00 ,  C12Q1/10 ,  C12Q1/34

CPC分类号： C12Q1/04 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52 ,  G01N2333/24 ,  Y10S435/849 ,  Y10S435/879

摘要： A test method and medium for quantitatively identifying and distinguishing biological materials in a test sample. A first biological material has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has specificity for one of the substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes. The first and second biological materials are capable of fermenting a sugar, and the third biological material does not ferment the sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon fermentation, resulting in the formation of a zone of a third color around the water insoluble compounds of the fermenting materials. Following incubation, the sample is examined for the presence of colonies of the first biological material, having the first color and an encircling zone of the third color; for colonies of the second material, having the second color and the colored zone; and for colonies of a third material having the first or second color, and not having the colored zone.

公开(公告)号：EP0842260B1

公开(公告)日：2002-11-13

申请号：EP96921676.1

申请日：1996-06-14

申请人： RCR Scientific, Inc.

发明人： ROTH, Jonathan, N. ,  BONTRAGER, Gordon

IPC分类号： C12M1/00

CPC分类号： B05C13/00 ,  B05C7/04 ,  C12M23/10

摘要： A plurality of petri dishes or other glass or plastic containers are coated on the inside bottom surface of the containers by passing the containers through a coating or layering apparatus. The apparatus includes an indexing conveyor to sequentially pass the open containers through a filling station where an amount of the coating material is applied while tilting the container to one side thereby distributing the material to the entire bottom surface of the container. The containers are conveyed to an aspirating station to aspirate excess material while tilting the container toward the aspirating nozzle. Subsequent to the aspiration step, the containers are tilted in the opposite direction to distribute the material in the container. The material is then dried and the containers stacked and packaged. The coating material includes metal ions such as calcium or other divalent metal ions, in a suitable carrier that is able to bond the bottom inside surface of the container. The coating material forms a dehydrated, solidified layer bonded onto the bottom surface of the container. The coating material can be a water/agar solution or a plastic resin in an appropriate solvent containing the calcium ions.

公开(公告)号：EP0897428B1

公开(公告)日：2007-06-06

申请号：EP97915186.7

申请日：1997-03-21

申请人： RCR Scientific, Inc.

发明人： ROTH, Jonathan, N. ,  BONTRAGER, Gordon, L.

IPC分类号： C12Q1/04 ,  C12Q1/54 ,  C12Q1/18 ,  C12Q1/02 ,  C12Q1/22 ,  C12N5/00 ,  C12N1/00 ,  C12Q1/10 ,  C12Q1/34

CPC分类号： C12Q1/04 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52 ,  G01N2333/24 ,  Y10S435/849 ,  Y10S435/879

摘要： A test method and medium for quantitatively identifying and differentiating biological materials in a test sample containing a plurality of different biological materials. A first biological material in the sample has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has enzyme specificity for one of the chromogenic substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes from the biological materials. The first and second biological materials are capable of fermenting a sugar, and the third biological material does not ferment the sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon acidification of a portion of the test medium due to fermentation of the sugar, which color change results in the formation of a colored zone around the water insoluble compounds of the sugar-fermenting biological materials, the colored zone being distinguishable from the colors of the water insoluble compounds.

公开(公告)号：EP0897428A1

公开(公告)日：1999-02-24

申请号：EP97915186.0

申请日：1997-03-21

申请人： RCR Scientific, Inc.

发明人： ROTH, Jonathan, N. ,  BONTRAGER, Gordon, L.

IPC分类号： C12Q1 ,  C12N1

CPC分类号： C12Q1/04 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52 ,  G01N2333/24 ,  Y10S435/849 ,  Y10S435/879

摘要： A test method and medium for quantitatively identifying and distinguishing biological materials in a test sample. A first biological material has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has specificity for one of the substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes. The first and second biological materials are capable of fermenting a sugar, and the third biological material does not ferment the sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon fermentation, resulting in the formation of a zone of a third color around the water insoluble compounds of the fermenting materials. Following incubation, the sample is examined for the presence of colonies of the first biological material, having the first color and an encircling zone of the third color; for colonies of the second material, having the second color and the colored zone; and for colonies of a third material having the first or second color, and not having the colored zone.