摘要:
A test method and medium for quantitatively identifying and distinguishing biological materials in a test sample. A first biological material has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has specificity for one of the substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes. The first and second biological materials are capable of fermenting a sugar, and the third biological material does not ferment the sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon fermentation, resulting in the formation of a zone of a third color around the water insoluble compounds of the fermenting materials. Following incubation, the sample is examined for the presence of colonies of the first biological material, having the first color and an encircling zone of the third color; for colonies of the second material, having the second color and the colored zone; and for colonies of a third material having the first or second color, and not having the colored zone.
摘要:
The invention concerns a nutritive gelosed culture medium for directly identifying pathogenic bacteria of the genus Listeria, said medium containing a synthetic chromogenous substrate specifically cleaved by phosphatidylinositol-specific phospholipase C (PIPLC).
摘要:
The invention concerns a culture medium for revealing of enterohaemorragic E. coli bacteria, particularly serotypes 0157 and/or 011, comprising a chromogenic substrate compound of the alpha -galactosidase enzyme. The invention likewise concerns a procedure for revealing enterohaemorragic E. coli in a sample.
摘要:
A temperature history indicator in which a reaction is initiated by blending a plurality of separate reagents is disclosed. The temperature history indicator characterized in that an enzyme and an enzyme substrate are employed as said reagents; said enzyme and said enzyme substrate undergo color reaction; said color reaction is carried out in the specified temperature range so as to form a specified color. The disposable temperature history indicator which can be directly attached onto food packages and is useful for the management as well as the maintenance in food sanitation in the temperature range which is suitable for the growth of bacteria.
摘要:
The invention provides a method for the rapid assessment of the degree of halitosis comprising the steps of: a) obtaining a sample of fluid and/or tissue from the oral cavity of a subject, b) assessing the amount of β-galactosidase present in said sample, c) determining the degree of halitosis in said subject, by comparing the result obtained in step b) with appropriate reference values.
摘要:
A temperature history indicator in which a reaction is initiated by blending a plurality of separate reagents is disclosed. The temperature history indicator characterized in that an enzyme and an enzyme substrate are employed as said reagents; said enzyme and said enzyme substrate undergo color reaction; said color reaction is carried out in the specified temperature range so as to form a specified color. The disposable temperature history indicator which can be directly attached onto food packages and is useful for the management as well as the maintenance in food sanitation in the temperature range which is suitable for the growth of bacteria.
摘要:
Die Erfindung betrifft Mittel und Verfahren zur Bestimmung von hydrolytischen Enzymen in flüssigen Proben mit Hilfe von Indoxylverbindungen und Oxidationsmitteln. Das Verfahren ist dadurch gekennzeichnet, daß man den mit einer Indoxylverbindung imprägnierten saugfähigen Träger mit der Probelösung benetzt, in die das Oxidationsmittel enthaltende Lösung eintaucht, inkubiert und reflektometrisch auswertet.
摘要:
An improved method for testing for the presence of a particular microorganism and/or group of microorganisms characterized by a particular enzyme is provided. The method uses a dye-forming and/or fluorogenic-generating substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate or fluorescent by-product when cleaved by the enzyme. The precipitate is concentrated on the polymer matrix and/or solid support member to create a visible reaction product, whereas the fluorescent by-product will normally be dispersed into the surrounding medium to some extent. The amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicator device comprising a dye-forming and/or fluorogenic-generating substrate throughout a polymer system or on the surface of a solid support member.