公开(公告)号：EP1290147A1

公开(公告)日：2003-03-12

申请号：EP00980811.4

申请日：2000-11-27

申请人： California Institute of Technology ,  Arnold, Frances ,  Petrounia, Ioanna P. ,  Sun, Lianhong

发明人： ARNOLD, Frances, H. ,  PETROUNIA, Ioanna P. ,  SUN, Lianhong

IPC分类号： C12N9/00 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12N15/102

摘要： This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantageously produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.

公开(公告)号：EP1290147A4

公开(公告)日：2004-12-08

申请号：EP00980811

申请日：2000-11-27

申请人： CALIFORNIA INST OF TECHN ,  ARNOLD FRANCES ,  PETROUNIA IOANNA P ,  SUN LIANHONG

发明人： ARNOLD FRANCES H ,  PETROUNIA IOANNA P ,  SUN LIANHONG

IPC分类号： C12N15/09 ,  C12N9/04 ,  C12N15/10 ,  C12N9/00 ,  C07H21/04 ,  C12N15/00

CPC分类号： C12N9/0006 ,  C12N15/102

摘要： This invention relates to the expression of improved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly oxidase enzymes. The enzymes are advantageously produced in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved expression, enhanced activity toward one or more substrates, and increased thermal stability. In a particular embodiment, the invention relates to improved expression of the galactose oxidase gene and galactose oxidase enzymes. GAO mutants that are highly active and/or thermostable are disclosed.