公开(公告)号：EP3464632A2

公开(公告)日：2019-04-10

申请号：EP17803363.5

申请日：2017-05-22

申请人： Tribiotica Llc

发明人： DUNN, Ian ,  LAWLER, Matthew

IPC分类号： C12Q1/68 ,  C07H21/04

公开(公告)号：EP3227476A1

公开(公告)日：2017-10-11

申请号：EP15865336.0

申请日：2015-12-02

申请人： Tribiotica Llc

发明人： DUNN, Ian ,  LAWLER, Matthew

IPC分类号： C40B40/00 ,  C40B20/00 ,  C12Q1/68

CPC分类号： C12Q1/6816 ,  C12N15/1093 ,  C12Q2533/107 ,  C12Q2563/107 ,  C40B20/00 ,  C40B40/00

摘要： The present disclosure is directed to methods and kits for identifying, enriching, and evaluating templated assembly reactants. Some embodiments disclose methods for identifying templated assembly targets by synthesizing templated assembly reactants, hybridizing the templated assembly reactants to target nucleic acids, performing a templated assembly reaction, and identifying the target nucleic acids that hybridized to the templated assembly reactants. Libraries of templated assembly reactants, a kit for identifying templated assembly targets, and a pair of templated assembly targets enriched from a library of chemically-ligated oligonucleotides spatially elicited (CLOSE) products are also disclosed.

摘要翻译： 本公开涉及用于鉴定，富集和评估模板化组装反应物的方法和试剂盒。 一些实施方案公开了通过合成模板化装配反应物，使模板化装配反应物与靶核酸杂交，进行模板化装配反应，以及鉴定与模板化装配反应物杂交的靶核酸来鉴定模板化组装靶的方法。 也公开了模板化装配反应物的文库，用于鉴定模板化装配目标的试剂盒和从化学连接的寡核苷酸的文库中富集的一对模板化装配目标（空间引发（CLOSE）产物）。

公开(公告)号：EP3004351B1

公开(公告)日：2018-08-08

申请号：EP14807845.4

申请日：2014-06-04

申请人： Tribiotica Llc

发明人： DUNN, Ian ,  LAWLER, Matthew

IPC分类号： C12N15/11 ,  C12N15/10 ,  C12Q1/68

CPC分类号： A61K47/549 ,  A61K39/0011 ,  A61K2039/5158 ,  A61K2039/6025 ,  C12N15/1068 ,  C12N15/111 ,  C12N15/1131 ,  C12N2310/351 ,  C12N2310/3513 ,  C12N2310/3517 ,  C12N2320/30 ,  C12Q1/6811 ,  C12N2310/321 ,  C12N2310/3521 ,  C12Q2543/10 ,  C12Q2523/109

摘要： The present disclosure is directed methods and products for synthesizing and using targeted templated assembly reactants comprising at least one nucleic acid recognition moiety, at least one selectively-reactive moiety, and at least one effector partial moiety. The nucleic acid recognition moiety can bind a target nucleic acid sequence within a sample. The nucleic acid recognition moiety also can bind the selectively-reactive moiety. Additionally, the effector partial moiety can bind the selectively-reactive moiety to produce an active effector structure. Also disclosed are methods of delivering the targeted templated assembly reactants and active effector structures formed from the targeted templated assembly reactants.

公开(公告)号：EP3004351A4

公开(公告)日：2016-12-21

申请号：EP14807845

申请日：2014-06-04

申请人： TRIBIOTICA LLC

发明人： DUNN IAN ,  LAWLER MATTHEW

IPC分类号： C12N15/11 ,  C12N15/10 ,  C12Q1/68

CPC分类号： A61K47/48092 ,  A61K39/0011 ,  A61K47/549 ,  A61K2039/5158 ,  A61K2039/6025 ,  C12N15/1068 ,  C12N15/111 ,  C12N15/1131 ,  C12N2310/351 ,  C12N2310/3513 ,  C12N2310/3517 ,  C12N2320/30 ,  C12Q1/6811 ,  C12N2310/321 ,  C12N2310/3521 ,  C12Q2543/10 ,  C12Q2523/109

摘要： The present disclosure is directed methods and products for synthesizing and using targeted templated assembly reactants comprising at least one nucleic acid recognition moiety, at least one selectively-reactive moiety, and at least one effector partial moiety. The nucleic acid recognition moiety can bind a target nucleic acid sequence within a sample. The nucleic acid recognition moiety also can bind the selectively-reactive moiety. Additionally, the effector partial moiety can bind the selectively-reactive moiety to produce an active effector structure. Also disclosed are methods of delivering the targeted templated assembly reactants and active effector structures formed from the targeted templated assembly reactants.

公开(公告)号：EP3004351A1

公开(公告)日：2016-04-13

申请号：EP14807845.4

申请日：2014-06-04

申请人： Tribiotica Llc

发明人： DUNN, Ian ,  LAWLER, Matthew

IPC分类号： C12N15/11

CPC分类号： A61K47/48092 ,  A61K39/0011 ,  A61K47/549 ,  A61K2039/5158 ,  A61K2039/6025 ,  C12N15/1068 ,  C12N15/111 ,  C12N15/1131 ,  C12N2310/351 ,  C12N2310/3513 ,  C12N2310/3517 ,  C12N2320/30 ,  C12Q1/6811 ,  C12N2310/321 ,  C12N2310/3521 ,  C12Q2543/10 ,  C12Q2523/109

摘要： The present disclosure is directed methods and products for synthesizing and using targeted templated assembly reactants comprising at least one nucleic acid recognition moiety, at least one selectively-reactive moiety, and at least one effector partial moiety. The nucleic acid recognition moiety can bind a target nucleic acid sequence within a sample. The nucleic acid recognition moiety also can bind the selectively-reactive moiety. Additionally, the effector partial moiety can bind the selectively-reactive moiety to produce an active effector structure. Also disclosed are methods of delivering the targeted templated assembly reactants and active effector structures formed from the targeted templated assembly reactants.