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    METHOD FOR PREPARING FETUIN AND POLYPEPTIDE TO INDUCE APOPTOSIS
    1.
    发明公开
    METHOD FOR PREPARING FETUIN AND POLYPEPTIDE TO INDUCE APOPTOSIS 审中-公开
    用于生产胎球蛋白和多肽诱导细胞凋亡

    公开(公告)号：EP1414472A1

    公开(公告)日：2004-05-06

    申请号：EP02749954.0

    申请日：2002-07-09

    申请人： Tsai, David

    发明人： Tsai, David

    IPC分类号： A61K35/14 ,  A61K38/04

    CPC分类号： C07K14/4747 ,  A61K33/30 ,  A61K38/1741 ,  C07K14/71

    摘要： The present invention provides for an improved method of preparing fetuin by using a chelating agent to remove inorganic ions , such as zinc , calcium, and barium, from fetuin and then, reloading the 'naked' fetuin with Zinc Acetate to form a product that is mainly Fetuin-Zinc. This improved method of preparing Fetuin-Zinc or supercharged zinc fetuin increases the effectiveness of inducing apoptosis in cancer cells by three to four times. A specific peptide fragment was then derived from this specially prepared zinc charged fetuin. This polypeptide strongly induced apoptosis in LNCaP (prostate cancer) and HT-29 (colon cancer) cells without affecting CCD 18 co (normal colon) cells, The in vitro tissue culture study demonstrated that the polypeptide is approximately ten times more potent than the parent molecule (full-length zinc charged fetuin) in inducing apoptosis.

    PROTEINS FOR CANCER CELL SPECIFIC INDUCTION OF APOPTOSIS AND METHOD FOR ISOLATION THEREOF
    4.
    发明公开
    PROTEINS FOR CANCER CELL SPECIFIC INDUCTION OF APOPTOSIS AND METHOD FOR ISOLATION THEREOF 审中-公开
    蛋白质的细胞凋亡，并为您绝缘癌细胞特异性入门程序

    公开(公告)号：EP1037919A1

    公开(公告)日：2000-09-27

    申请号：EP98964142.8

    申请日：1998-12-18

    申请人： Tsai, David ,  Yu, Jenny

    发明人： Tsai, David ,  Yu, Jenny

    IPC分类号： C07K14/47 ,  C07K14/435 ,  A61K38/17

    CPC分类号： C07K14/4747 ,  A61K38/00 ,  C07K14/71

    摘要： The present invention provides the methods to isolate the proteins specifically induced apoptosis (programmed cell death) in prostate cancer cells (LNCAP), leukemia cells (HL-60), and breast cancer cells (MCF-70), but without effect in normal human lung fibroblast cells (CCD 39 Lu). P-1 has no effect on breast cancer cells. Five proteins have been isolated from the conditioned media of culture cells: (1) Apogen P-1: the proteins (Apogen P-1a, Apogen P-1b and Apogen P-1c) isolated from the conditioned medium of XC cells are able to induce apoptosis in prostate cancer cells (LNCAP) without effect in normal human lung fibroblast (CCD 39 Lu), colon cancer (T84), breast cancer (MCF-7) and leukemia (HL-60) cells. (2) Apogen P-2: the protein isolated from the conditioned medium of C3H1OT1/2 cells is able to induce apoptosis in prostate cancer cells (LNCAP) and breast cancer (MCF-7) without effect in normal human lung fibroblast (CCD 39 Lu) and colon cancer (T84) cells. (3) Apogen L: the protein isolated from the conditioned medium of XC cells is able to induce apoptosis in leukemia cells (HL-60), and breast cancer (MCF-7) without effect in normal human lung fibroblast (CCD 39 Lu), colon cancer (T84) and prostate cancer (LNCAP) cells. The invention may lead to the discovery of a novel class of anticancer drug that aims at prostate cancer, breast cancer, leukemia and other cancers by inducing apoptosis in cancer cells without affecting normal cells.