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    Device for cultivating cells
    1.
    发明公开
    Device for cultivating cells 审中-公开
    培养细胞的装置

    公开(公告)号：EP2955220A1

    公开(公告)日：2015-12-16

    申请号：EP14305888.1

    申请日：2014-06-12

    Applicant: UNIVERSITE PARIS DESCARTES ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE -CNRS- ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM) ,  Laboratoire Mabio International ,  UNIVERSITE PARIS-SUD 11

    Inventor： Couraud, Olivier ,  Ganeshamoorthy, Kayathiri ,  Lachman, Vincent ,  Barik, Samir ,  Uzan, Georges ,  Boyer, Julie

    IPC: C12M1/00 ,  C12M3/00 ,  C12M1/42 ,  C12M1/32 ,  G01N33/50 ,  C12M1/12 ,  C12M3/06 ,  B01L3/00

    CPC classification number: C12M23/44 ,  B01L3/502 ,  B01L2300/0681 ,  B01L2300/0877 ,  C12M23/12 ,  C12M23/16 ,  C12M23/34 ,  C12M25/04 ,  C12M35/02 ,  C12M35/04 ,  G01N33/502

    Abstract: The invention related to a device for cultivating cells comprising:
    - a lower part comprising at least two lower compartments, a lower wall separating the two lower compartments, and a semi-porous membrane covering the lower compartments, the semi-porous membrane comprising an upper surface intended to receive the cells;
    - a first upper part and a second upper part intended to be fitted onto the lower part, in order to obtain selectively:
    - a first configuration of the device wherein the first upper part is fitted onto the lower part, the first upper part forming an upper compartment wherein a culture medium can flow, in order to apply shear stress to the cells;
    - a second configuration of the device wherein the second upper part is fitted onto the lower part, the second upper part comprising at least two upper compartments, a wall separating the two upper compartments, and injection openings for injecting a substance to be tested in each upper compartment, the wall of the second upper part facing the wall of the lower element and being in contact with the semi-porous membrane so as to prevent a leakage of the injected substance from an upper compartment to another.

    Abstract translation: 本发明涉及培养细胞的装置，其包括： - 下部，其包含至少两个下部隔室，分隔两个下部隔室的下部壁和覆盖下部隔室的半多孔膜，所述半多孔膜包括上部 旨在接收细胞的表面; - 旨在装配到下部上的第一上部和第二上部，以便选择性地获得： - 装置的第一构造，其中第一上部装配到下部上，第一上部形成 其中培养基可以流动的上部隔室，以便向细胞施加剪切应力; - 装置的第二构型，其中第二上部装配到下部上，第二上部包括至少两个上部隔室，隔开两个上部隔室的壁以及用于将待测物质注入每个上部室的注射开口 上隔室，第二上部的壁面向下部元件的壁并且与半多孔隔膜接触，以防止注入物从上隔室泄漏到另一隔室。

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