公开(公告)号：EP2198303A1

公开(公告)日：2010-06-23

申请号：EP08830981.0

申请日：2008-09-10

申请人： Nickerson, Cheryl A. ,  Wilson, James W. ,  Ott, C. Mark ,  Nauman, Eric A. ,  Schurr, Michael J. ,  Nelman-Gonzalez, Mayra A. ,  Sarker, Shameema

发明人： Nickerson, Cheryl A. ,  Wilson, James W. ,  Ott, C. Mark ,  Nauman, Eric A. ,  Schurr, Michael J. ,  Nelman-Gonzalez, Mayra A. ,  Sarker, Shameema

IPC分类号： G01N33/569

CPC分类号： C12Q1/025 ,  C12N1/36 ,  C12N1/38 ,  C12N15/01

摘要： This invention is directed to applying a low sedimental fluid shear environment to manipulate microorganisms, and to microorganisms and compositions obtained based on such manipulation. Specifically, the present invention provides methods of modifying a molecular genetic or phenotypic characteristic (e.g., virulence, stress resistance or biofilm formation) of a microorganism by culturing in a low sedimental shear environment. One or more ion concentrations in the culture can be modulated in order to inhibit or amplify the extent of the modification. The present invention also provides microorganisms obtained from a low sedimental shear culture, which exhibit modified and desirable phenotypic characteristics, as well as therapeutic, vaccineand bioindustrial products prepared from such microorganisms. Further, the present invention provides methods for identifying molecules that modulate responses of a microorganism to a low sedimental shear environment and for determining the relevance of such molecules to pathogenenicity of the microorganism.

公开(公告)号：EP2198303A4

公开(公告)日：2011-08-03

申请号：EP08830981

申请日：2008-09-10

申请人： NICKERSON CHERYL A ,  WILSON JAMES W ,  OTT C MARK ,  NAUMAN ERIC A ,  SCHURR MICHAEL J ,  NELMAN-GONZALEZ MAYRA A ,  SARKER SHAMEEMA

发明人： NICKERSON CHERYL A ,  WILSON JAMES W ,  OTT C MARK ,  NAUMAN ERIC A ,  SCHURR MICHAEL J ,  NELMAN-GONZALEZ MAYRA A ,  SARKER SHAMEEMA

IPC分类号： G01N33/569

CPC分类号： C12Q1/025 ,  C12N1/36 ,  C12N1/38 ,  C12N15/01