公开(公告)号：EP3244992A4

公开(公告)日：2018-08-01

申请号：EP16737637

申请日：2016-01-07

申请人： 10X GENOMICS INC

发明人： HARDENBOL PAUL ,  PATEL PRANAV ,  HINDSON BENJAMIN ,  WYATT PAUL ,  BJORNSON KEITH ,  WU INDIRA ,  BELHOCINE KAMILA

IPC分类号： B01J19/00

CPC分类号： C12P19/34 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12Q1/6806 ,  C40B20/04 ,  C12Q2525/121 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2535/122 ,  C12Q2563/149

摘要： This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.