公开(公告)号：EP2885418A4

公开(公告)日：2016-03-02

申请号：EP13829413

申请日：2013-08-13

申请人： 10X GENOMICS INC

发明人： HINDSON BENJAMIN ,  SAXONOV SERGE

IPC分类号： C12P19/34 ,  A61K9/50 ,  A61K9/52

CPC分类号： C12Q1/6806 ,  B01J19/0046 ,  B01L3/502715 ,  B01L3/508 ,  B01L3/523 ,  B01L2200/0647 ,  B01L2400/0677 ,  C12N15/1065 ,  C12Q2535/122 ,  C12Q2563/159

摘要： This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.

公开(公告)号：EP3161162A4

公开(公告)日：2018-01-10

申请号：EP15812053

申请日：2015-06-26

申请人： 10X GENOMICS INC

发明人： SCHNALL-LEVIN MICHAEL ,  JAROSZ MIRNA ,  HINDSON CHRISTOPHER ,  NESS KEVIN ,  SAXONOV SERGE ,  HINDSON BENJAMIN ,  ZHENG GRACE X Y ,  MARKS PATRICK ,  STUELPNAGEL JOHN

IPC分类号： C12Q1/68 ,  C12N15/10 ,  C40B40/06

CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/156 ,  G06F19/22 ,  C12Q2535/122 ,  C12Q2537/16 ,  C12Q2563/159 ,  C12Q2565/629

摘要： The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

公开(公告)号：EP2954065A4

公开(公告)日：2016-08-24

申请号：EP14748569

申请日：2014-02-07

申请人： 10X GENOMICS INC

发明人： HINDSON BENJAMIN ,  SAXONOV SERGE ,  NESS KEVIN ,  HARDENBOL PAUL ,  HINDSON CHRISTOPHER ,  MASQUELIER DONALD ,  JAROSZ MIRNA ,  SCHNALL-LEVIN MICHAEL

IPC分类号： C12P19/34

CPC分类号： C12N15/1065 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/112 ,  G01N33/5436

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

摘要翻译： 本公开提供了用于多核苷酸处理的组合物，方法，系统和装置。 这种多核苷酸处理可用于多种应用，包括多核苷酸测序。

公开(公告)号：EP3244992A4

公开(公告)日：2018-08-01

申请号：EP16737637

申请日：2016-01-07

申请人： 10X GENOMICS INC

发明人： HARDENBOL PAUL ,  PATEL PRANAV ,  HINDSON BENJAMIN ,  WYATT PAUL ,  BJORNSON KEITH ,  WU INDIRA ,  BELHOCINE KAMILA

IPC分类号： B01J19/00

CPC分类号： C12P19/34 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12Q1/6806 ,  C40B20/04 ,  C12Q2525/121 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2535/122 ,  C12Q2563/149

摘要： This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：EP3129143A4

公开(公告)日：2017-04-26

申请号：EP15776968

申请日：2015-04-09

申请人： 10X GENOMICS INC

发明人： BHARADWAJ RAJIV ,  NESS KEVIN ,  MITRA DEBKISHORE ,  MASQUELIER DONALD ,  MAKAREWICZ ANTHONY ,  HINDSON CHRISTOPHER ,  HINDSON BENJAMIN ,  SAXONOV SERGE

IPC分类号： B01L3/00

CPC分类号： B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N35/085 ,  G01N2035/00237 ,  G01N2035/1034

公开(公告)号：EP3013957A4

公开(公告)日：2016-08-24

申请号：EP14817610

申请日：2014-06-26

申请人： 10X GENOMICS INC

发明人： HINDSON BENJAMIN ,  HINDSON CHRISTOPHER ,  SCHNALL-LEVIN MICHAEL ,  NESS KEVIN ,  JAROSZ MIRNA ,  MASQUELIER DONALD ,  SAXONOV SERGE ,  MERRILL LANDON ,  PRICE ANDREW ,  HARDENBOL PAUL ,  LI YUAN

IPC分类号： C12N15/10 ,  C12Q1/68 ,  G01N33/566 ,  G06F19/20

CPC分类号： C12Q1/6869 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2565/514

摘要： This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

摘要翻译： 本公开提供了用于样品处理的方法和组合物，特别是用于测序应用。 在本公开内容中包括珠组合物，例如连接到大量含有条形码的寡核苷酸的珠的不同文库。 通常，本文提供的珠粒是可降解的。 例如，它们可以含有对还原剂敏感的二硫键。 本文提供的方法包括制备条形码珠的文库的方法以及将珠与样品组合的方法，例如通过使用微流体装置。

公开(公告)号：EP2954104A4

公开(公告)日：2016-08-24

申请号：EP14749595

申请日：2014-02-07

申请人： 10X GENOMICS INC

发明人： HINDSON BENJAMIN ,  JAROSZ MIRNA ,  HARDENBOL PAUL ,  SCHNALL-LEVIN MICHAEL ,  NESS KEVIN ,  SAXONOV SERGE

IPC分类号： C40B50/06 ,  C40B20/00

CPC分类号： C12N15/1065 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/112 ,  G01N33/5436

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

摘要翻译： 本公开提供了用于多核苷酸处理的组合物，方法，系统和装置。 这种多核苷酸处理可用于多种应用，包括多核苷酸测序。

公开(公告)号：EP2931919A4

公开(公告)日：2016-08-24

申请号：EP13862194

申请日：2013-12-12

申请人： 10X GENOMICS INC

发明人： HINDSON BENJAMIN ,  SAXONOV SERGE ,  NESS KEVIN ,  HARDENBOL PAUL

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

摘要翻译： 本公开提供了用于多核苷酸处理的组合物，方法，系统和装置。 这种多核苷酸处理可用于多种应用，包括多核苷酸测序。