公开(公告)号：EP1941874A4

公开(公告)日：2012-03-28

申请号：EP06797037

申请日：2006-08-30

申请人： AJINOMOTO KK

发明人： TAGAMI TOMOYUKI ,  TABATA TOMOYUKI ,  UMEZAWA TSUTOMU ,  SUZUKI YASUO

IPC分类号： A61K31/198 ,  A61K31/19 ,  A61K31/56 ,  A61P1/00 ,  A61P1/04 ,  A61P29/00

CPC分类号： A61K31/19 ,  A61K31/198 ,  A61K31/56

公开(公告)号：EP0950665A4

公开(公告)日：2004-05-06

申请号：EP97942202

申请日：1997-09-26

申请人： AJINOMOTO KK

发明人： TAKAHARA YOSHIYUKI ,  SATO HARUYA ,  HAYASHI EIKO ,  YATAGAI MASANOBU ,  SUZUKI MANABU ,  TABATA TOMOYUKI ,  EJIMA CHIEKO

IPC分类号： A61K38/00 ,  C07H15/04 ,  C07H15/08 ,  C07K14/00 ,  C07K14/52 ,  C07K14/55 ,  C07K14/56 ,  A61P1/16

CPC分类号： C07H15/08 ,  A61K38/00 ,  C07H15/04 ,  C07K14/00 ,  C07K14/52 ,  C07K14/55 ,  C07K14/56

摘要： Modified physiologically active protein which can be produced by reacting a physiologically active protein having a molecular weight falling within the range of from 1 x 10 to 2 x 10 and carrying at least one Gln residue serving as the substitute of transglutaminase with a branched ligand consisting of an amino acid derivative having a lower binding affinity to the asialoglycoprotein receptor located on hepatic parenchymal cells than that to asialoorsomucoid and carrying an amino group and a galactose (Gal) or N-acetylgalactosamine (GalNAc) group serving as the substrate of transglutaminase in the presence of a transglutaminase originating in a microorganism to thereby form an amide bond between the gamma -carboxyamide group of the glutamate residue in the physiologically active protein and the terminal amino group in the branched ligand; and a process for producing the same.