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    MODIFIED BETA-GALACTOSIDASE
    1.
    发明公开
    MODIFIED BETA-GALACTOSIDASE 审中-公开

    公开(公告)号：EP3184633A4

    公开(公告)日：2017-12-27

    申请号：EP15834600

    申请日：2015-08-13

    申请人： AMANO ENZYME INC

    发明人： ISHIKAWA KAZUHIKO ,  ISHIHARA SATORU ,  YAMAGUCHI SHOTARO

    IPC分类号： C12N9/38 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/09 ,  C12P19/04

    CPC分类号： C12N9/2402 ,  C12N15/09 ,  C12P19/04 ,  C12P19/14 ,  C12Y302/01023

    摘要： The present invention has a purpose of providing a technique for increasing the heat resistance of a ²-galactosidase. According to the present invention, in a reference ²-galactosidase amino acid sequence which shows a 90% or more identity to the amino acid sequence of SEQ ID NO: 4, proline is substituted for one or more amino acids selected from the group consisting of the following amino acids: (1) an amino acid corresponding to lysine at position 166 of the amino acid sequence of SEQ ID NO: 4, (2) an amino acid corresponding to glycine at position 307 of the amino acid sequence of SEQ ID NO: 4, and (3) an amino acid corresponding to alanine at position 833 of the amino acid sequence of SEQ ID NO: 4.

    COMPOSITION HAVING IMPROVED HEAD STABILITY AND USE THEREOF
    2.
    发明公开
    COMPOSITION HAVING IMPROVED HEAD STABILITY AND USE THEREOF 有权
    ZUSAMMENSETZUNG MIT VERBESSERTERKOPFSTABILITÄTUND VERWENDUNG DAVON

    公开(公告)号：EP2749633A4

    公开(公告)日：2015-07-01

    申请号：EP12825652

    申请日：2012-08-24

    申请人： AMANO ENZYME INC

    发明人： IDO HIROKI ,  OBARA TAKAKIYO ,  YAMAGUCHI SHOTARO

    IPC分类号： C12C5/02

    CPC分类号： C12C5/02 ,  C12C5/00 ,  C12C5/004 ,  C12C7/00

    摘要： The object is to provide a composition effective for improving foam stability of a beer-based sparkling beverage and the like. Provided is a composition containing a protein or a polypeptide produced by using a protease and a transglutaminase to treat a protein material as an active component.

    摘要翻译： 本发明的目的是提供一种有效提高啤酒类起泡饮料等的泡沫稳定性的组合物。 本发明提供含有通过使用蛋白酶和转谷氨酰胺酶处理蛋白质材料作为活性成分而产生的蛋白质或多肽的组合物。

    METHOD FOR DESIGNING MUTANT ENZYME, METHOD FOR PREPARING MUTANT ENZYME, AND MUTANT ENZYME
    3.
    发明公开
    METHOD FOR DESIGNING MUTANT ENZYME, METHOD FOR PREPARING MUTANT ENZYME, AND MUTANT ENZYME 审中-公开
    处理对突变酶的建设，方法生产经酶突变体和酶突变体

    公开(公告)号：EP2351837A4

    公开(公告)日：2013-01-02

    申请号：EP09812834

    申请日：2009-08-12

    申请人： AMANO ENZYME INC

    发明人： HASHIZUME RYOTA ,  MIKAMI BUNZO ,  MATSUBARA HIROTAKA ,  MATSUNAGA AKIKO ,  YAMAGUCHI SHOTARO

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12P21/02

    CPC分类号： C12N9/80 ,  C12N9/48

    摘要： An object is to provide a novel method of improving an enzyme capable of deamidating a protein. A mutant enzyme is designed by the following steps: (1) specifying one or more amino acids selected from the following group, namely, consisting of an amino acid corresponding to the amino acid at position 35, an amino acid corresponding to the amino acid at position 38, an amino acid corresponding to the amino acid at position 39, an amino acid corresponding to the amino acid at position 40, an amino acid corresponding to the amino acid at position 41, an amino acid corresponding to the amino acid at position 42, an amino acid corresponding to the amino acid at position 43, an amino acid corresponding to the amino acid at position 45, an amino acid corresponding to the amino acid at position 46, an amino acid corresponding to the amino acid at position 49, an amino acid corresponding to the amino acid at position 79, an amino acid corresponding to the amino acid at position 80, an amino acid corresponding to the amino acid at position 81, an amino acid corresponding to the amino acid at position 82, an amino acid corresponding to the amino acid at position 83, an amino acid corresponding to the amino acid at position 84, an amino acid corresponding to the amino acid at position 103, an amino acid corresponding to the amino acid at position 104, an amino acid corresponding to the amino acid at position 105, an amino acid corresponding to the amino acid at position 106, an amino acid corresponding to the amino acid at position 117, an amino acid corresponding to the amino acid at position 142, an amino acid corresponding to the amino acid at position 143, an amino acid corresponding to the amino acid at position 146, an amino acid corresponding to the amino acid at position 166, and an amino acid corresponding to the amino acid at position 185 in an amino acid sequence set forth in SEQ ID NO: 2, in a protein deamidase (an enzyme to be mutated); and (2) constructing an amino acid sequence having substitution of the amino acid(s) specified in the step (1) by another amino acid(s) or having deletion of the amino acid(s) specified in the step (1) using the amino acid sequence for an enzyme to be mutated as a base sequence.

    摘要翻译： 一个目的是提供一种酶，该酶能脱酰胺的蛋白质的改进的新方法。 的突变体酶是通过以下步骤设计的：（1）指定从以下组中选择的一个或多个氨基酸，即，一个氨基酸在35位对应于所述氨基酸由......组成氨基酸对应于所述氨基酸在 位置38至位置39的氨基酸对应于氨基酸第40位到氨基酸对应于氨基酸41位氨基酸对应于氨基酸氨基酸对应于氨基酸42位 在氨基酸对应于氨基酸第43位到氨基酸第45位对应于氨基酸第46位到氨基酸对应于所述氨基酸在49位氨基酸对应于氨基酸 氨基酸位置79对应于该氨基酸在80位氨基酸对应于氨基酸到81位氨基酸对应于氨基酸氨基酸的氨基酸CORRESPONDING 在位置82至氨基酸位置83对应于该氨基酸的氨基酸在位置84对应于该氨基酸在103位氨基酸对应于氨基酸氨基酸对应于所述氨基酸在位置 104到在位置105的氨基酸对应于氨基酸氨基酸对应于氨基酸106位氨基酸对应于氨基酸第117位在位置142的氨基酸对应于氨基酸， 在氨基酸对应于所述氨基酸在143位的氨基酸在146位对应于该氨基酸在166位氨基酸对应于氨基酸，并且在在位置185的氨基酸对应于氨基酸 氨基酸序列示于SEQ ID NO：2，在一个蛋白质脱酰胺酶（待突变的酶）; 和（2）在氨基酸序列构建的步骤中指定具有在步骤（1）的另一种氨基酸（一个或多个）中指定的氨基酸（一个或多个）的取代或具有氨基酸（一个或多个）的缺失（1）使用 在酶的氨基酸序列被突变为碱基序列。