摘要:
The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspect of the present invention aims to provide a cloning method for hESCs and hiPSCs based on culture of dispersed single cells utilizing shear stress. The above problem is solved by providing a method for culturing dispersed single pluripotent cells, wherein the dispersed single cells are cultured under laminar flow conditions.
摘要:
A cell culture device comprising: a channel having an inlet end for the introduction of a liquid and a bottom surface, wherein said channel is configured for the flow through of said liquid; and a plurality of recesses for containing cells, which are formed on said bottom surface of said channel; wherein said plurality of recesses are closely arranged on said bottom surface of said channel.
摘要:
A fluid agitation method is provided, whereby a swirling flow is generated in a trace amount of fluid, thereby agitating the fluid. The fluid agitation method includes introducing the fluid into an agitation chamber (3) including a wall having an uneven mass distribution, and applying oscillation (F) to the wall with frequencies varying in a predetermined frequency range. The uneven mass distribution of the wall is attained, for example, by arranging a plurality of thickened portions (11 to 18) of different thicknesses in a ring.
摘要:
The present invention provides a novel target analysis chip and analysis method for directly detecting a target such as a microRNA without performing PCR. The target analysis chip according to the invention includes a substrate, and the substrate includes a reaction section at which a sample containing a target is made to react with a reagent, a detection section at which label detection is performed, and a flow channel that communicates the reaction section with the detection section, in which the reagent includes a carrier; the carrier has a labeled probe immobilized thereon, the labeled probe being configured to bind to the target, and the carrier being configured to form a conjugate with the target; the flow channel includes a movement controller configured to control movement of the carrier from the reaction section to the detection section; the movement controller includes a hydrophobic inner wall in the flow channel; and, due to the movement controller, the conjugate is made to move to the detection section through the flow channel when a centrifugal force (C2), that is larger than a resistance force (R) caused by the hydrophobicity of the flow channel, is applied,.
摘要:
An analytical cartridge comprising a minute flow path is provided. The minute flow path includes flow paths (43a,43c,43d) connected to each other directly at respective first ends. The liquid feeding direction of blood S in the minute flowpath is controlled by creating a high-pressure state at a second end of the flow path (43a) located across the blood (S), while creating a low-pressure state or a closed state at a second end of the flow paths (43d,43c).
摘要:
A novel target analysis method and target analyzing chip are provided to directly detect a target, such as microRNA, without performing PCR. The target analysis method of the present embodiment includes: a process in which a target in a sample, a labeled probe that binds to the target, and a carrier-binding probe that binds to the target and to a carrier, contact each other, and the target, the labeled probe, and the carrier-binding probe react to bind to each other to form a first bound body; a process in which the first bound body and the carrier contact each other, and the first bound body and the carrier react to bind to each other to form a second bound body; a process to concentrate the carrier; and a process in which the target in the sample is analyzed by detecting a label of the labeled probe bound to the concentrated carrier.