公开(公告)号：EP1708564A4

公开(公告)日：2007-03-07

申请号：EP04749444

申请日：2004-03-26

申请人： AVIGENICS INC

发明人： RAPP JEFFREY ,  CHRISTMANN LEANDRO ,  HARVEY ALEX ,  LEAVITT MARKLEY

IPC分类号： A01K67/00 ,  A01K67/027 ,  C12N15/00 ,  C12N15/85 ,  C12N15/90

CPC分类号： C12N15/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/02 ,  C12N15/8509

摘要： The invention provides methods for integrating a heterologous polynucleotide into the genome of an avian cell. The methods deliver to an avian cell a polynucleotide and a source of integrase activity that mediates recombination between the polynucleotide and the genomic DNA of the avian cell. The invention provides modified avian or artificial chromosomes as vectors to shuttle transgenes or gene clusters into an avian genome. Another aspect of the invention are avian cells genetically modified with a transgene vector. One cell line for the delivery and integration of a transgene comprises a heterologous attP site and, optionally, a region for expressing the integrase. Methods are also included for the production of a heterologous polypeptide by transgenic avian tissue involve integrating a heterologous polynucleotide into the avian genome. The present invention also relates to methods of producing transgenic chickens which include introducing into an avian cell a nucleic acid comprising a transgene and an integrase activity in addition to a cationic polymer and/or a nuclear localization signal and introducing the avian cell into a recipient avian wherein the recipient avian produces an offspring which includes the transgene. Also included are methods of dispersing a nucleic acid in a cell.

公开(公告)号：EP1608219A4

公开(公告)日：2007-03-14

申请号：EP04716170

申请日：2004-03-01

申请人： AVIGENICS INC

发明人： HARVEY ALEX ,  LEAVITT MARKLEY C ,  CHRISTMANN LEANDRO

IPC分类号： A01K20060101 ,  A01K67/00 ,  A01K67/027 ,  C12N15/85 ,  C12N15/86

CPC分类号： A01K67/0275 ,  A01K2217/072 ,  A01K2227/30 ,  A01K2267/01 ,  C12N15/8509 ,  C12N2800/204 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2830/40 ,  C12N2830/90 ,  C12N2840/20 ,  C12N2840/203

摘要： The invention provides methods for integrating a heterologous polynucleotide into the genome of an avian cell. The methods deliver to an avian cell a polynucleotide and a source of integrase activity that mediates recombination between the polynucleotides and the genomic DNA of the avian cell. The invention provides modified avian or artificial chromosomes as vectors to shuttle transgenes or gene clusters into an avian genome. Another aspect of the invention are avian cells genetically modified with a transgene vector. One cell line for the delivery and integration of a transgene comprises a heterologous attP site and, optionally, a region for expressing the integrase. Methods are also included for the production of a heterologous polypeptide by transgenic avian tissue involve integrating a heterologous polynucleotide into the avian genome. Subsequently, a mature transgenic avian is derived by transferring the transgenic blastodermal cells to an embryo and allowing that embryo to develop fully.

公开(公告)号：EP1623035A4

公开(公告)日：2006-08-30

申请号：EP03816706

申请日：2003-12-11

申请人： UNIV GEORGIA RES FOUND ,  AVIGENICS INC

发明人： IVARIE ROBERT ,  LEAVITT MARKLEY C ,  KARNUAH ARTHUR B ,  RAPP JEFF ,  CHRISTMANN LEANDRO

IPC分类号： C12N15/85 ,  C07K16/00

CPC分类号： C12N15/8509 ,  A01K67/0278 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/11 ,  C07K2317/21 ,  C12N2799/02 ,  C12N2800/10 ,  C12N2800/204 ,  C12N2800/206 ,  C12N2830/90

摘要： The present invention provides isolated and recombinant avian nucleic acid molecules comprising at least one avian MAR and an avian nucleic acid sequence encoding an ovalbumin transcriptional regulatory region. The isolated nucleic acid of the present invention is useful for reducing chromosomal positional effects upon the transcription of a transgene operably linked to the ovalbumin transcriptional regulatory region and transfected into a recipient avian cell The recombinant nucleic cid molecules of the present invention may further comprise a polyadenylation signal sequence or an avian 3' domain, and optionally, an internal ribosome entry site for expression of an operably linked heterologous nucleic acid insert in a transfected avian cell.

摘要翻译： 本发明提供了分离的和重组的禽核酸分子，其包含至少一个禽MAR和编码卵清蛋白转录调节区的禽核酸序列。 本发明的分离的核酸可用于降低染色体位置效应，所述染色体位置效应对与卵清蛋白转录调控区有效连接的转基因进行转录并转染至受体禽类细胞中。本发明的重组核酸分子可进一步包含聚腺苷酸化 信号序列或禽类3'结构域，并且任选地，用于在转染的禽细胞中表达可操作地连接的异源核酸插入物的内部核糖体进入位点。

公开(公告)号：EP1438401A4

公开(公告)日：2005-03-09

申请号：EP02775895

申请日：2002-09-18

申请人： AVIGENICS INC

发明人： RAPP JEFFREY C ,  CHRISTMANN LEANDRO

IPC分类号： A01K67/027 ,  C07K16/00 ,  C12N15/85 ,  C12N15/00

CPC分类号： A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/20 ,  C07K2317/23 ,  C12N15/8509 ,  C12N2799/027 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/46 ,  C12N2830/90 ,  C12N2840/203

摘要： This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.

公开(公告)号：EP1365643A4

公开(公告)日：2004-06-30

申请号：EP02718905

申请日：2002-02-06

申请人： AVIGENICS INC

发明人： CHRISTMANN LEANDRO

IPC分类号： A01K67/027 ,  C12M3/00 ,  C12N15/89 ,  A01K45/00

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12M35/00 ,  G02B21/32

摘要： The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.