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    CONCATEMERS OF DIFFERENTIALLY EXPRESSED MULTIPLE GENES
    8.
    发明授权
    CONCATEMERS OF DIFFERENTIALLY EXPRESSED MULTIPLE GENES 有权
    连环差异表达的多基因

    公开(公告)号：EP1360308B1

    公开(公告)日：2007-10-10

    申请号：EP02709978.7

    申请日：2002-01-25

    申请人： Evolva Ltd.

    发明人： GOLDSMITH, Neil ,  SORENSEN, Alexandra, M., P., SantAna ,  NIELSEN, Soren, V., S. ,  NAESBY, Michael

    IPC分类号： C12N15/66 ,  C12N15/10

    CPC分类号： C12N15/81 ,  C12N15/66 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/20 ,  C12N2800/206

    摘要： In the present invention are disclosed concatemers of concatenated expression cassettes and vectors that enable the synthesis of such concatemers. The main purpose of these concatemers is the controllable and co-ordinated expression of large numbers of heterologous genes in a single host. Furthermore, the invention relates to a concatemers of cassettes of nucleotide sequences and a method for preparing the concatemers. In a further aspect, the invention relates to transgenic host cells comprising at least one concatemer according to the invention, as well as to a method for preparatin the transgenic host cells. Finally, the invention relates to a vector comprising a cassette of nucleotides, a method for preparing said vector, a nucleotide library comprising at least two primary vectors each comprising a cassette of nucleotides, a method for prepararing the library.

    GENE MANIPULATION AND EXPRESSION USING GENOMIC ELEMENTS
    10.
    发明授权
    GENE MANIPULATION AND EXPRESSION USING GENOMIC ELEMENTS 无效
    基因操作和表达的基因组元素

    公开(公告)号：EP0584214B1

    公开(公告)日：2004-07-28

    申请号：EP92911887.5

    申请日：1992-05-05

    申请人： CELL GENESYS, INC.

    发明人： SHERWIN, Stephen ,  KLAPHOLZ, Sue ,  SKOULTCHI, Arthur

    IPC分类号： C12N15/85 ,  C12N15/81 ,  C12P21/06 ,  C12N15/53 ,  C12N1/19 ,  C12N5/10

    CPC分类号： C07K14/535 ,  C07K14/59 ,  C12N15/81 ,  C12N15/85 ,  C12N2800/206 ,  C12N2840/44

    摘要： Expression of mammalian target genes is achieved by employing chromosomal target DNA, either native primary cells or YACs in a yeast host, where the YACs include a fragment of a mammalian chromosome, the fragment comprising the target gene. Employing homologous recombination, an amplifiable gene is integrated into the mammalian fragment at a site to allow for amplification. In the same step, or one or more steps, as desired, the mammalian gene and/or the transcriptional system may be modified by in vivo mutagenesis. The resulting construct from homologous recombination may then be transformed into a mammalian expression host and integrated into the host genome, either randomly or by homologous recombination. The amplifiable gene may then be amplified by an appropriate agent providing for multiple copies of the target gene and the expression host grown to provide for high yields of the desired wild-type or modified protein.