公开(公告)号：EP2336175A1

公开(公告)日：2011-06-22

申请号：EP10179020.2

申请日：2001-12-05

申请人： Abbott Laboratories

发明人： Lou, Sheng, C. ,  Hunt, Jeffrey, C. ,  Konrath, John, G. ,  Qiu, Xiaoxing ,  Scheffel, James, W. ,  Tyner, Joan, D.

IPC分类号： C07K16/10 ,  C12N5/20 ,  G01N33/577 ,  G01N33/569 ,  G01N33/68 ,  C07K14/16

CPC分类号： C07K16/1054 ,  C07K7/08 ,  C07K14/005 ,  C07K14/161 ,  C07K2317/34 ,  C12N2740/16211 ,  C12N2740/16222 ,  G01N33/56983 ,  G01N33/68

摘要： The present invention relates to novel monoclonal antibodies which may be used in the detection of Human Immunodeficiency Virus (HIV). These antibodies exhibit an unusually high degree of sensitivity, a remarkably broad range of specificity, and bind to novel shared, non-cross-reactive epitopes. In particular, the monoclonal antibodies of the present invention may be utilized to detect HIV-1 antigen and HIV-2 core antigen in a patient sample.

公开(公告)号：EP2336175B1

公开(公告)日：2017-01-18

申请号：EP10179020.2

申请日：2001-12-05

申请人： ABBOTT LABORATORIES

发明人： Lou, Sheng, C. ,  Hunt, Jeffrey, C. ,  Konrath, John, G. ,  Qiu, Xiaoxing ,  Scheffel, James, W. ,  Tyner, Joan, D.

IPC分类号： C07K16/10 ,  C12N5/20 ,  G01N33/577 ,  G01N33/569 ,  G01N33/68 ,  C07K14/16 ,  C07K7/08 ,  C07K14/005

CPC分类号： C07K16/1054 ,  C07K7/08 ,  C07K14/005 ,  C07K14/161 ,  C07K2317/34 ,  C12N2740/16211 ,  C12N2740/16222 ,  G01N33/56983 ,  G01N33/68

公开(公告)号：EP2765206A1

公开(公告)日：2014-08-13

申请号：EP14162068.2

申请日：2008-10-22

申请人： Abbott Laboratories

发明人： Lou, Sheng, C. ,  Chau, Kurt, H. ,  Konrath, John, G.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6804 ,  B03C1/01 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/26 ,  C12Q1/703 ,  C12Q2563/143 ,  C12Q2563/131 ,  C12Q2563/125

摘要： A method for improving the sensitivity of an immunoassay by a factor of at least 10 to about 25, and perhaps greater. Furthermore, in addition to increasing the sensitivity of the assay, the method of this invention also improves assay specificity. In order to match or improve upon sensitivity of 0.2 pg/mL for an analyte, this invention provides an immunoassay involving amplification of a signal, e.g., a chemiluminescent signal, a specific binding member, e.g., a monoclonal antibody, and microparticle separation, e.g., magnetic microparticle separation, from large volumes of sample (e.g., from about 0.2 to about 3 mL). Such an immunoassay can be carried out with an automated immunoassay analyzers, e.g., an automated immunoassay analyzer in the ARCHITECT TM family of analyzers.

摘要翻译： 将免疫测定的灵敏度提高至少10至25，甚至更大的方法。 此外，除了增加测定的灵敏度之外，本发明的方法还提高了测定特异性。 为了匹配或提高分析物的0.2pg / mL的灵敏度，本发明提供了涉及扩增信号的免疫测定，例如化学发光信号，特异性结合成员，例如单克隆抗体和微粒分离，例如 ，磁性微粒分离，从大量的样品（例如，约0.2至约3mL）。 这样的免疫测定可以用自动化免疫分析仪进行，例如ARCHITECT TM系列分析仪中的自动化免疫分析仪。