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公开(公告)号：EP3597772A1
公开(公告)日：2020-01-22
申请号：EP19179386.8
申请日：2014-04-17
IPC分类号： C12Q1/68 , C40B40/06 , C40B50/06 , C12Q1/6869 , C12N15/10
摘要： The present invention provides an approach to increase the effective read length of commercially available sequencing platforms to several kilobases and be broadly applied to obtain long sequence reads from mixed template populations. A method for generating extended sequence reads of long DNA molecules in a sample, comprising the steps of: assigning a specific barcode sequence to each template DNA molecule in a sample to obtain barcode-tagged molecules; amplifying the barcode-tagged molecules; fragmenting the amplified barcode-tagged molecules to obtain barcode-containing fragments; juxtaposing the barcode-containing fragments to random short segments of the original DNA template molecule during the process of generating a sequencing library to obtain demultiplexed reads; and assembling the demultiplexed reads to obtain extended sequence reads for each DNA template molecule, is disclosed.
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公开(公告)号：EP2986741B1
公开(公告)日：2019-07-24
申请号：EP14785836.9
申请日：2014-04-17
IPC分类号： C12Q1/6869 , C12N15/10
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公开(公告)号：EP2986741A2
公开(公告)日：2016-02-24
申请号：EP14785836.9
申请日：2014-04-17
CPC分类号： C12N15/1065 , C12Q1/6869 , C12Q2531/125 , C12Q2535/122 , C12Q2537/143 , C12Q2537/165 , C12Q2563/185
摘要： The present invention provides an approach to increase the effective read length of commercially available sequencing platforms to several kilobases and be broadly applied to obtain long sequence reads from mixed template populations. A method for generating extended sequence reads of long DNA molecules in a sample, comprising the steps of: assigning a specific barcode sequence to each template DNA molecule in a sample to obtain barcode-tagged molecules; amplifying the barcode-tagged molecules; fragmenting the amplified barcode-tagged molecules to obtain barcode-containing fragments; juxtaposing the barcode-containing fragments to random short segments of the original DNA template molecule during the process of generating a sequencing library to obtain demultiplexed reads; and assembling the demultiplexed reads to obtain extended sequence reads for each DNA template molecule, is disclosed. Also disclosed are methods systems and software for assembling paired end sequence reads to produce extended reads.
摘要翻译： 本发明提供了一种将商业上可用的测序平台的有效阅读长度增加到几千碱基的方法，并且广泛地应用于从混合模板群获得长序列阅读。 一种用于产生样品中长DNA分子的延伸序列读数的方法，包括以下步骤：将特定条形码序列分配给样品中的每个模板DNA分子以获得条形码标记的分子; 扩增条形码标记的分子; 将扩增的条形码标记的分子片段化以获得包含条形码的片段; 在产生测序文库的过程中将含有条形码的片段并置到原始DNA模板分子的随机短片段上以获得解多重读取; 并且组装解多重读取以获得每种DNA模板分子的延伸序列读数。
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