公开(公告)号：EP1571223A2

公开(公告)日：2005-09-07

申请号：EP05004829.7

申请日：2005-03-04

申请人： Ajinomoto Co., Inc.

发明人： Takagi, Hiroshi ,  Nakamori, Shigeru ,  Wada, Masaru ,  Mori, Hirotada, c/o Nara Institute of

IPC分类号： C12P13/12 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，其被修饰以使得半胱氨酸脱硫酸酶活性降低或消除。 该细菌在培养基中培养以产生并导致培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

公开(公告)号：EP1571223B1

公开(公告)日：2010-01-20

申请号：EP05004829.7

申请日：2005-03-04

申请人： Ajinomoto Co., Inc.

发明人： Takagi, Hiroshi ,  Nakamori, Shigeru ,  Wada, Masaru ,  Mori, Hirotada, c/o Nara Institute of

IPC分类号： C12P13/12 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60

CPC分类号： C12P13/12

公开(公告)号：EP1571223A3

公开(公告)日：2005-11-30

申请号：EP05004829.7

申请日：2005-03-04

申请人： Ajinomoto Co., Inc.

发明人： Takagi, Hiroshi ,  Nakamori, Shigeru ,  Wada, Masaru ,  Mori, Hirotada, c/o Nara Institute of

IPC分类号： C12P13/12 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力并含有编码O-乙酰丝氨酸硫化酶B或MalY调节蛋白的基因的埃希氏菌属细菌产生L-半胱氨酸，所述基因被修饰以使半胱氨酸脱硫酶活性降低或消除。 将细菌在培养基中培养以在培养基中产生并引起L-半胱氨酸蓄积，并从培养基中收集L-半胱氨酸。