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    L-Cysteine producing bacterium and method for producing l-cysteine
    2.
    发明公开
    L-Cysteine producing bacterium and method for producing l-cysteine 有权
    生产L-半胱氨酸的细菌和生产L-半胱氨酸的方法

    公开(公告)号：EP1298200A3

    公开(公告)日：2003-07-02

    申请号：EP02020588.6

    申请日：2002-09-17

    申请人： Ajinomoto Co., Inc.

    发明人： Takagi, Hiroshi ,  Wada, Masaru ,  Nakamori, Shigeru

    IPC分类号： C12N1/20 ,  C12R1/19 ,  C12P13/12

    CPC分类号： C12R1/19 ,  C12N9/88 ,  C12P13/12

    摘要： L-Cysteine is produced by culturing a bacterium belonging to the genus Escherichia having an L-cysteine producing ability and modified so that cystathionine-β-lyase activity or cystathionine-β-lyase activity and tryptophanase activity should be reduced or eliminated in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium.

    摘要翻译： L-半胱氨酸是通过培养具有L-半胱氨酸生产能力的埃希氏杆菌属细菌并进行修饰使得在培养基中降低或消除胱硫醚-β-裂解酶活性或胱硫醚-β-裂合酶活性和色氨酸酶活性 在培养基中产生并积累L-半胱氨酸并从培养基中收集L-半胱氨酸。

    Method for producing L-glutamic acid by fermentation and a method for producing a new microorganism
    6.
    发明公开
    Method for producing L-glutamic acid by fermentation and a method for producing a new microorganism 失效
    一种用于通过发酵生产L-谷氨酸，和用于生产新微生物的方法的过程。

    公开(公告)号：EP0030391A2

    公开(公告)日：1981-06-17

    申请号：EP80107790.0

    申请日：1980-12-10

    申请人： AJINOMOTO CO., INC.

    发明人： Tsuchida, Takayasu ,  Nakamori, Shigeru

    IPC分类号： C12P13/14 ,  C12N15/00

    CPC分类号： C12P13/14 ,  C12N15/52 ,  C12R1/19 ,  Y10S435/848 ,  Y10S435/849

    摘要： A method for producing L-glutamic acid by fermentation is provided, comprising culturing in a culture medium an L-glutamic acid producing microorganism of the genus Escherichia. According to this method a microorganism is cultered which is obtained by incorporating into a recipient strain of the genus Escherichia a hybrid plasmid having inserted therein a DNA fragment with the genetic information controlling the L-glutamic acid production, which fragment is derived from a donor strain of the genus Escherichia which is capable of producing L-glutamic acid.
    The invention further comprises a method for producing a new microorganism of the genus Escherichia having high productivity for L-glutamic acid, according to which chromosomal DNA is obtained from a mutant strain of the genus Eschierichia which is capable of producing L-glutamic acid, the obtained DNA is treated with a restrio- tion endonuclease to obtain the DNA-fragment with the genetic information responsible for the L-glutamic acid synthesis, the DNA fragment is inserted into a plasmid obtained from a microorganism of the genus Eschierichia and the recombinant plasmid is incorporated into a microorganism of the genus Escherichia as a recipient organism.

    摘要翻译： 提供了一种用于通过发酵生产L-谷氨酸的方法，在埃希氏菌属的L-谷氨酸生产微生物的培养基，其包含培养。 。根据该方法的微生物的所有其通过掺入一个杂交质粒插入有与所述遗传信息控制L-谷氨酸生产的DNA片段的埃希氏杆菌属的受体菌株获得横纹肌，该片段从供体菌株的 的埃希氏杆菌属的所有其能够产生L-谷氨酸的。 ... 本发明还包括用于生产埃希氏杆菌属具有高生产率的新微生物的L-谷氨酸的方法，雅丁到哪个染色体DNA从该属Eschierichia所有其能够产生L-中的突变株中获得 谷氨酸，将获得的DNA进行处理用限制性内切酶，以获得与负责L-谷氨酸合成的遗传信息的DNA片段，该DNA片段被插入到从属和Eschierichia的微生物中获得的质粒 重组质粒掺入到埃希氏杆菌属的微生物作为受体生物体。

    L-cysteine producing bacterium and method for producing L-cysteine
    7.
    发明公开
    L-cysteine producing bacterium and method for producing L-cysteine 有权
    L-Cystein herstellendes Bakterium und Verfahren zur Herstellung von L-cystein

    公开(公告)号：EP1645623A1

    公开(公告)日：2006-04-12

    申请号：EP05024341.9

    申请日：2002-09-17

    申请人： Ajinomoto Co., Inc.

    发明人： Takagi, Hiroshi ,  Wada, Masaru ,  Nakamori, Shigeru

    IPC分类号： C12N1/20 ,  C12R1/19 ,  C12P13/12

    CPC分类号： C12R1/19 ,  C12N9/88 ,  C12P13/12

    摘要： L-Cysteine is produced by culturing a bacterium belonging to the genus Escherichia having an L-cysteine producing ability and modified so that cystathionine-β-lyase activity or cystathionine-β-lyase activity and tryptophanase activity should be reduced or eliminated in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium.

    摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏杆菌属细菌产生L-半胱氨酸，并进行修饰，使得在培养基中减少或消除胱硫醚-2裂解酶活性或胱硫醚-β裂解酶活性和色氨酸酶活性 在培养基中产生并积累L-半胱氨酸，并从培养基中收集L-半胱氨酸。

    L-cysteine-producing microorganism and a method for producing L-cysteine
    9.
    发明公开
    L-cysteine-producing microorganism and a method for producing L-cysteine 有权
    Verfahren zur Herstellung von L-Cystein，und L-Cystein-produzierender Mikroorganismus

    公开(公告)号：EP1571223A2

    公开(公告)日：2005-09-07

    申请号：EP05004829.7

    申请日：2005-03-04

    申请人： Ajinomoto Co., Inc.

    发明人： Takagi, Hiroshi ,  Nakamori, Shigeru ,  Wada, Masaru ,  Mori, Hirotada, c/o Nara Institute of

    IPC分类号： C12P13/12 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60

    CPC分类号： C12P13/12

    摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

    摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，其被修饰以使得半胱氨酸脱硫酸酶活性降低或消除。 该细菌在培养基中培养以产生并导致培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。