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    METHOD OF PROLIFERATING PLURIPOTENT STEM CELL
    1.
    发明公开
    METHOD OF PROLIFERATING PLURIPOTENT STEM CELL 有权
    VERFAHREN ZUR PROLIFEATION EINER PLURPOTENTEN STAMMZELLE

    公开(公告)号：EP1734112A1

    公开(公告)日：2006-12-20

    申请号：EP05727500.0

    申请日：2005-03-23

    申请人： Daiichi Asubio Pharma Co., Ltd. ,  Fukuda, Keiichi ,  Akaike, Toshihiro

    发明人： AKAIKE, Toshihiro ,  FUKUDA, Keiichi ,  NAGAOKA, Masato ,  KOSHIMIZU, Uichi;

    IPC分类号： C12N5/06 ,  C12N5/08 ,  C07K14/47 ,  C12N15/09

    CPC分类号： C12N5/0606 ,  C12N2533/50

    摘要： A novel growth method is provided for pluripotent stem cells such as ES cells. The method of the invention is a pluripotent stem cell growing method and gene transfer method in which pluripotent stem cells are cultured under conditions that maintain their undifferentiated state and pluripotency, the method being characterized by using a liquid medium and a culturing vessel having immobilized or coated on a substrate solid phase surface a molecule which is adhesive to the pluripotent stem cells in a fixed concentration, to grow the pluripotent stem cells in a dispersed state while maintaining their undifferentiated state and pluripotency, without using feeder cells, or to transfer and express a gene therein.

    摘要翻译： 为多能干细胞如ES细胞提供了一种新的生长方法。 本发明的方法是多能干细胞生长方法和基因转移方法，其中多能干细胞在保持其未分化状态和多能性的条件下培养，该方法的特征在于使用液体培养基和具有固定或涂覆的培养容器 在底物固相表面上以固定浓度与多能干细胞粘合的分子，以分散状态生长多能干细胞，同时保持其未分化状态和多能性，而不使用饲养细胞，或转移和表达 基因。