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    METHODS AND COMPOSITIONS FOR MODIFYING A TARGETED LOCUS
    7.
    发明公开
    METHODS AND COMPOSITIONS FOR MODIFYING A TARGETED LOCUS 审中-公开
    VERFAHREN UND ZUSAMMENSETZUNGEN ZUR MODIFIZIERUNG VON EINES GEZIELTEN LOCUS

    公开(公告)号：EP3152312A1

    公开(公告)日：2017-04-12

    申请号：EP15729724.3

    申请日：2015-06-05

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： AUERBACH, Wojtek ,  FRENDEWEY, David ,  DROGUETT, Gustavo ,  GAGLIARDI, Anthony ,  KUNO, Junko ,  VALENZUELA, David M.

    IPC分类号： C12N15/90

    摘要： Methods and compositions are provided for modifying one or more target loci in a cell. Such methods comprise providing a cell comprising a first polynucleotide encoding a first selection marker operably linked to a first promoter active in the cell, wherein the first polynucleotide further comprises a first recognition site for a first nuclease agent. A first nuclease agent is introduced into a cell, wherein the first nuclease agent induces a nick or double-strand break at the first recognition site. Further introduced into the cell is a first targeting vector comprising a first insert polynucleotide flanked by a first and a second homology arm that correspond to a first and a second target site located in sufficient proximity to the first recognition site. At least one cell is then identified comprising in its genome the first insert polynucleotide integrated at the target locus.

    摘要翻译： 提供了用于修饰细胞中一个或多个靶基因座的方法和组合物。 这样的方法包括提供包含编码第一选择标记的第一多核苷酸的细胞，所述第一选择标记可操作地连接到细胞中活性的第一启动子，其中所述第一多核苷酸还包含第一核酸酶试剂的第一识别位点。 将第一核酸酶试剂引入细胞中，其中第一核酸酶试剂在第一识别位点诱导切口或双链断裂。 进一步引入细胞中的是第一靶向载体，其包含侧翼为第一和第二同源性臂的第一插入片段，其对应于位于第一识别位点附近的第一和第二靶位点。 然后鉴定至少一个细胞，其在其基因组中包含整合在靶标位点的第一插入多核苷酸。

    METHODS AND COMPOSITIONS FOR MODIFYING A TARGETED LOCUS
    8.
    发明公开
    METHODS AND COMPOSITIONS FOR MODIFYING A TARGETED LOCUS 审中-公开

    公开(公告)号：EP3708671A1

    公开(公告)日：2020-09-16

    申请号：EP20155939.0

    申请日：2015-06-05

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： AUERBACH, Wojtek ,  FRENDEWEY, David ,  DROGUETT, Gustavo ,  GAGLIARDI, Anthony ,  KUNO, Junko ,  VALENZUELA, David

    IPC分类号： C12N15/90

    摘要： Methods and compositions are provided for modifying one or more target loci in a cell. Such methods comprise providing a cell comprising a first polynucleotide encoding a first selection marker operably linked to a first promoter active in the cell, wherein the first polynucleotide further comprises a first recognition site for a first nuclease agent. A first nuclease agent is introduced into a cell, wherein the first nuclease agent induces a nick or double-strand break at the first recognition site. Further introduced into the cell is a first targeting vector comprising a first insert polynucleotide flanked by a first and a second homology arm that correspond to a first and a second target site located in sufficient proximity to the first recognition site. At least one cell is then identified comprising in its genome the first insert polynucleotide integrated at the target locus.