公开(公告)号：EP1243652A2

公开(公告)日：2002-09-25

申请号：EP02009293.8

申请日：1992-09-17

申请人： Amgen Inc.,

发明人： Lin, Leu-Fen H. ,  Collins, Franklin D. ,  Doherty, Daniel H. ,  Lile, Jack

IPC分类号： C12N15/12 ,  C07K14/475 ,  A61K38/18 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C07K16/18 ,  A61K48/00 ,  A61P25/00 ,  A61P25/16 ,  A61P25/28

CPC分类号： A61K9/0085 ,  A61K38/00 ,  C07K14/475 ,  Y10S530/809 ,  Y10S530/839

摘要： A novel neurotrophic factor referred to as glial derived neurotrophic factor (GDNF) has been identified and isolated from serum free growth conditioned medium of B49 glioblastoma cells. Rat and human genes encoding GDNF have been cloned and sequenced. A gene enconding GDNF has been subcloned into a vector, and the vector has been used to transform a host cell in order to produce biologically active GDNF in a recombinant DNA process.

摘要翻译： 已经鉴定了一种称为神经胶质神经营养因子（GDNF）的新型神经营养因子，并从B49胶质母细胞瘤细胞的无血清生长条件培养基中分离出来。 编码GDNF的大鼠和人基因已被克隆并测序。 将包含GDNF的基因亚克隆到载体中，并且该载体已经用于转化宿主细胞，以便在重组DNA过程中产生生物活性的GDNF。

公开(公告)号：EP1243652A3

公开(公告)日：2003-03-26

申请号：EP02009293.8

申请日：1992-09-17

申请人： Amgen Inc.,

发明人： Lin, Leu-Fen H. ,  Collins, Franklin D. ,  Doherty, Daniel H. ,  Lile, Jack

IPC分类号： C12N15/12 ,  C07K14/475 ,  A61K38/18 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C07K16/18 ,  A61K48/00 ,  A61P25/00 ,  A61P25/16 ,  A61P25/28

CPC分类号： A61K9/0085 ,  A61K38/00 ,  C07K14/475 ,  Y10S530/809 ,  Y10S530/839

摘要： A novel neurotrophic factor referred to as glial derived neurotrophic factor (GDNF) has been identified and isolated from serum free growth conditioned medium of B49 glioblastoma cells. Rat and human genes encoding GDNF have been cloned and sequenced. A gene enconding GDNF has been subcloned into a vector, and the vector has been used to transform a host cell in order to produce biologically active GDNF in a recombinant DNA process.