公开(公告)号：EP3406623A1

公开(公告)日：2018-11-28

申请号：EP17741374.7

申请日：2017-01-17

申请人： Asahi Kasei Medical Co., Ltd.

发明人： YOKOYAMA, Yoshiro ,  TANIGUCHI, Hiroki

IPC分类号： C07K1/18 ,  B01D15/36 ,  B01J20/281 ,  G01N30/00 ,  G01N30/88

CPC分类号： B01D15/36 ,  B01J20/281 ,  C07K1/18 ,  G01N30/00 ,  G01N30/88

摘要： The present invention provides a method for purifying a protein, comprising: providing a solution containing a monomer and aggregates of the protein of interest; a purification step of removing the aggregates of the protein of interest using a cation-exchange chromatographic support media to obtain a purified solution of the monomer, the cation-exchange chromatographic support media comprising at least one type of weak cation-exchange group and having a cation-exchange group density higher than 30 mmol/L; and a virus removal step of removing viruses from the purified solution using a virus removal membrane having a virus logarithmic reduction value of 3 or more.

公开(公告)号：EP3639911A1

公开(公告)日：2020-04-22

申请号：EP18818937.7

申请日：2018-06-05

申请人： Asahi Kasei Medical Co., Ltd.

发明人： IWASAKI, Takuma ,  YOKOYAMA, Yoshiro

IPC分类号： B01D61/14 ,  B01D61/16 ,  B01D61/58 ,  B01D63/08 ,  B01D65/02 ,  B01D69/12 ,  B01D71/34 ,  B01D71/56 ,  B01D71/68 ,  A61K38/00 ,  A61K39/395 ,  A61P31/12 ,  B01D65/08 ,  C07K16/06 ,  C07K16/00

摘要： A method for filtering a protein-containing liquid containing protein at a concentration of 20 mg/mL or more and 100 mg/mL or less, the method including a prefiltration step of filtering the protein-containing liquid by a prefilter having a pore size of 0.08 µm to 0.25 µm and including a hydrophobic resin, and a virus removal step of filtering the protein-containing liquid by a virus removal membrane including a synthetic polymer, after the prefiltration step, wherein the protein-containing liquid before conducting the prefiltration step includes 0.25 g or more of a trimer or higher multimer of the proteins having an average diameter of less than 100 nm, per 1 m 2 of the virus removal membrane.

公开(公告)号：EP2987801B1

公开(公告)日：2019-06-12

申请号：EP14785266.9

申请日：2014-04-15

申请人： Asahi Kasei Medical Co., Ltd.

发明人： TANIGUCHI, Hiroki ,  GOTO, Masako ,  KOGUMA, Ichiro ,  YOKOYAMA, Yoshiro

IPC分类号： B01D15/36 ,  B01J39/26 ,  C07K1/18 ,  G01N30/30 ,  G01N30/54 ,  G01N30/96

公开(公告)号：EP2987801A1

公开(公告)日：2016-02-24

申请号：EP14785266.9

申请日：2014-04-15

申请人： Asahi Kasei Medical Co., Ltd.

发明人： TANIGUCHI, Hiroki ,  GOTO, Masako ,  KOGUMA, Ichiro ,  YOKOYAMA, Yoshiro

IPC分类号： C07K1/18 ,  B01D15/04 ,  B01D15/08 ,  B01J20/24 ,  B01J20/26 ,  B01J20/281 ,  B01J20/30 ,  B01J20/34 ,  B01J39/04 ,  B01J39/26 ,  C07K1/22 ,  C08F291/00 ,  G01N30/26 ,  G01N30/54 ,  G01N30/88

CPC分类号： C07K1/22 ,  B01D15/362 ,  B01D15/3809 ,  B01J39/05 ,  B01J39/17 ,  B01J39/20 ,  B01J39/26 ,  B01J49/06 ,  C07K1/18 ,  C07K16/00 ,  G01N30/30 ,  G01N30/54 ,  G01N30/96 ,  G01N2030/8813

摘要： A method for purifying a biologically active substance from a solution mixture containing impurities and the biologically active substance, in which an ion exchange chromatography carrier comprising a matrix and a copolymer containing at least N-isopropylacrylamide as a monomer unit and immobilized to a surface of the matrix is used, and the solution mixture is allowed to flow through a container storing the carrier at a uniform temperature, thereby recovering the biologically active substance.

摘要翻译： 一种从含有杂质和生物活性物质的溶液混合物中纯化生物活性物质的方法，其中包含基质和至少含有N-异丙基丙烯酰胺作为单体单元的共聚物的离子交换色谱载体固定在 使溶液混合物在均匀的温度下流过容纳载体的容器，从而回收生物活性物质。