公开(公告)号：EP4371663A2

公开(公告)日：2024-05-22

申请号：EP23220315.8

申请日：2017-03-30

申请人： Cytiva BioProcess R&D AB

发明人： MALOISEL, Jean-Luc ,  VESTIN, Ida ,  LIND, Ola ,  SÖDERMAN, Tobias, E.

IPC分类号： B01J20/289

CPC分类号： B01D15/3804 ,  B01J20/267 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3208 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3278 ,  B01J20/3285 ,  B01J20/3293 ,  B01J2220/5420130101 ,  B01D15/327 ,  B01D15/361 ,  B01D15/3847 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J39/26 ,  B01J41/20 ,  B01J47/02

摘要： The invention discloses a separation matrix for purification of biomacromolecules, comprising a plurality of particles (1) having a core region (2) and a shell region (3), wherein:
a) said shell region is accessible to a target biomacromolecule;
b) said core region is less accessible to the target biomacromolecule than the shell region; and
c) the core region comprises a grafted polymer comprising residues of at least one polymerizable monomer.

公开(公告)号：EP3188816A4

公开(公告)日：2018-05-16

申请号：EP15838269

申请日：2015-08-19

申请人： EMD MILLIPORE CORP

发明人： AMARA JOHN PAUL ,  BOYLE JOHN ,  YAVORSKY DAVID ,  CACACE BENJAMIN

IPC分类号： B01D15/08 ,  B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/281 ,  B01J20/285 ,  B01J20/32 ,  B01J39/19 ,  B01J39/26 ,  B01J41/13 ,  B01J41/20 ,  B01J47/127 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C12N7/00

CPC分类号： B01J20/285 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01J20/26 ,  B01J20/28007 ,  B01J20/28023 ,  B01J20/28028 ,  B01J20/281 ,  B01J20/3208 ,  B01J20/3278 ,  B01J20/3293 ,  B01J39/19 ,  B01J39/26 ,  B01J41/13 ,  B01J41/20 ,  B01J47/127 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C12N7/00

摘要： Chromatography media including a high surface area thermoplastic porous nanofiber and an ion-exchange ligand functionality on the surface of the fiber. The porous nanofibers display a convoluted structure that is comprised of discrete bundles of highly entangled nanofibrils that may be fibrillated or ridged. The porous fibers can be prepared through the extraction of a dissolvable mineral or polymeric porogen that is embedded into the fiber during its manufacture in a melt extrusion process.

公开(公告)号：EP2942353B1

公开(公告)日：2018-05-09

申请号：EP15159525.3

申请日：2012-08-09

申请人： EMD Millipore Corporation

发明人： Bian, Nanying ,  Gillespie, Christopher ,  Stone, Matthew ,  Kozlov, Mikhail ,  Chen, Jie ,  Siwak, Martin

IPC分类号： C07K1/16 ,  C07K1/20 ,  B01D15/26 ,  B01J20/282

CPC分类号： C07K1/36 ,  B01D15/125 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J20/20 ,  B01J20/282 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  C07K1/16 ,  C07K1/20 ,  C07K16/00 ,  C07K2317/10

摘要： A flow-through process for purifying a target molecule from a Protein A eluate comprising the steps of:(i) contacting the eluate recovered from a Protein A chromatography column with activated carbon, (ii) contacting the flow-through sample from step (i) with an anion exchange chromatography media, (iii) contacting the flow-through sample from step (ii) with a cation exchange chromatography media and (iv) obtaining the flow-through sample from step (iii) comprising the target molecule, wherein the eluate flows continuously through steps (i)-(iii) and wherein level of one or more impurities in the flow-through sample after step (iii) is lower than the level in the eluate in step (i). The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.

公开(公告)号：EP3268101A1

公开(公告)日：2018-01-17

申请号：EP16708577.8

申请日：2016-02-26

申请人： Dow Global Technologies LLC ,  Rohm and Haas Company

发明人： GISCH, Daryl J. ,  EICHER, Christopher R. ,  PEASE, Stephen ,  PU, Gongwei

IPC分类号： B01D15/36 ,  B01J39/20 ,  B01J39/26 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  C13K1/00 ,  C13K11/00

CPC分类号： B01D15/362 ,  B01J39/05 ,  B01J39/20 ,  B01J39/26 ,  C13K1/00 ,  C13K1/08 ,  C13K11/00

摘要： A method for chromatographically separating a first saccharide from a liquid eluent comprising the first saccharide and a second saccharide by passing the liquid eluent through a bed including a polymeric macroporous alkylene-bridged resin in calcium form.

公开(公告)号：EP2639239B1

公开(公告)日：2017-09-06

申请号：EP13275053.0

申请日：2013-03-12

申请人： Merck Patent GmbH

发明人： Kozlov, Mikhail ,  Cataldo, William ,  Potty, Ajish ,  Galipeau, Kevin ,  Hamzik, James ,  Umana, Joaquin ,  Peeck, Lars

IPC分类号： C07K1/16 ,  C07K1/18

CPC分类号： C07K1/22 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J39/20 ,  B01J39/26 ,  B01J41/20 ,  B01J47/026 ,  C07K1/165 ,  C07K1/18 ,  C07K16/00

公开(公告)号：EP3210210A1

公开(公告)日：2017-08-30

申请号：EP15790195.0

申请日：2015-10-22

申请人： The University Of Hull

发明人： ARCHIBALD, Stephen James ,  HE, Ping ,  HASWELL, Stephen John ,  PAMME, Nicole ,  BROWN, Nathan Joel ,  TARN, Mark Duncan ,  ALEXANDER, Richard ,  ESFAHANI, Mohammad Mehdi Nasr

IPC分类号： G21G1/00 ,  B01J19/00

CPC分类号： B01J20/28042 ,  B01D15/322 ,  B01D15/362 ,  B01D15/363 ,  B01J19/0093 ,  B01J20/02 ,  B01J20/103 ,  B01J20/22 ,  B01J20/28061 ,  B01J20/28092 ,  B01J20/282 ,  B01J20/283 ,  B01J20/291 ,  B01J20/3007 ,  B01J20/3078 ,  B01J39/26 ,  B01J41/20 ,  B01J2219/0079 ,  B01J2219/00792 ,  B01J2219/00813 ,  B01J2219/00824 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00916 ,  B01J2220/82 ,  B29C45/14 ,  B29C71/02 ,  B29C2071/022 ,  B29K2105/0058 ,  B29K2709/00 ,  G01N30/96 ,  G01N33/15 ,  G21G1/001

摘要： The present invention relates to monolithic bodies, uses thereof and processes for the preparation thereof. Certain embodiments of the present invention relate to the use of a monolithic body in the preparation of a radioactive substance, for example a radiopharmaceutical, as part of a microfluidic flow system and a process for the preparation of such a monolithic body.

摘要翻译： 本发明涉及整体式体，其用途及其制备方法。 本发明的某些实施方案涉及整体式体在制备放射性物质例如放射性药物中的用途，作为微流体流动系统的一部分和用于制备这样的整体式体的方法。

公开(公告)号：EP3188816A1

公开(公告)日：2017-07-12

申请号：EP15838269.7

申请日：2015-08-19

申请人： EMD Millipore Corporation

发明人： AMARA, John Paul ,  BOYLE, John ,  YAVORSKY, David ,  CACACE, Benjamin

IPC分类号： B01D15/08

CPC分类号： B01J20/285 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01J20/26 ,  B01J20/28007 ,  B01J20/28023 ,  B01J20/28028 ,  B01J20/281 ,  B01J20/3208 ,  B01J20/3278 ,  B01J20/3293 ,  B01J39/19 ,  B01J39/26 ,  B01J41/13 ,  B01J41/20 ,  B01J47/127 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C12N7/00

摘要： Chromatography media including a high surface area thermoplastic porous nanofiber and an ion-exchange ligand functionality on the surface of the fiber. The porous nanofibers display a convoluted structure that is comprised of discrete bundles of highly entangled nanofibrils that may be fibrillated or ridged. The porous fibers can be prepared through the extraction of a dissolvable mineral or polymeric porogen that is embedded into the fiber during its manufacture in a melt extrusion process.

摘要翻译： 色谱介质包括高表面积热塑性多孔纳米纤维和纤维表面上的离子交换配体官能团。 多孔纳米纤维显示出卷曲结构，其由分散的高度缠结的纳米纤丝束构成，所述纳米纤丝可以被纤维化或脊形成。 多孔纤维可以通过在熔体挤出过程中提取在其制造过程中嵌入纤维中的可溶解矿物或聚合物致孔剂来制备。

公开(公告)号：EP2077158B1

公开(公告)日：2017-05-31

申请号：EP08170635.0

申请日：2008-12-04

申请人： LANXESS Deutschland GmbH

发明人： Vanhoorne, Pierre ,  Wedemeyer, Hans-Jürgen

IPC分类号： B01J39/20 ,  B01J39/26 ,  C08F8/36 ,  C02F1/42 ,  C07C37/20 ,  B01J31/10 ,  C08F257/02 ,  C13B20/14

CPC分类号： B01J39/20 ,  B01J31/10 ,  B01J39/26 ,  C02F2001/425 ,  C07C37/20 ,  C08F8/36 ,  C13B20/148 ,  C07C39/16 ,  C08F212/08

公开(公告)号：EP2997073B1

公开(公告)日：2017-04-19

申请号：EP14725897.4

申请日：2014-04-22

申请人： 3M Innovative Properties Company

发明人： WENDLAND, Michael S.

IPC分类号： C08G75/00 ,  C08J5/00 ,  C08G61/00 ,  C08G63/66 ,  C08L81/00

CPC分类号： B01J39/20 ,  B01J39/26 ,  B01J41/14 ,  B01J41/20 ,  C08F8/32 ,  C08F8/36 ,  C08F12/30 ,  C08F12/34 ,  C08F212/34 ,  C08F2800/10 ,  C08G75/00 ,  C08L81/00 ,  C08F212/36

公开(公告)号：EP3094392A1

公开(公告)日：2016-11-23

申请号：EP15702074.4

申请日：2015-01-16

申请人： Genzyme Corporation

发明人： GODAWAT, Rahul ,  WARIKOO, Veena ,  PATIL, Rohan ,  KONSTANTINOV, Konstantin ,  RYAKALA, Venkat Kishore

IPC分类号： B01D15/18 ,  B01D15/20 ,  A61L2/08

CPC分类号： B01J20/3441 ,  A61L2/081 ,  B01D15/1864 ,  B01D15/20 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/265 ,  B01J20/281 ,  B01J39/26 ,  B01J41/12 ,  B01J41/20 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  Y10T29/49826

摘要： Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein.

摘要翻译： 本文提供了降低色谱树脂的生物负载（例如灭菌）的方法，其包括将包含包含色谱树脂和至少一种抗氧化剂和/或螯合剂的组合物的容器暴露于足以减少生物负载的γ射线剂量 的容器和色谱树脂，其中至少一种抗氧化剂和/或螯合剂的存在量足以改善在暴露于γ-照射剂量之后/之后色谱树脂的结合能力的损失。 还提供了减少的生物负载层析柱，包括还原的生物负载层析树脂，包含色谱树脂和至少一种螯合剂和/或抗氧化剂的组合物，使用这些还原的生物负载层析色谱柱之一进行减少的生物负载层析色谱的方法，以及整合的封闭 以及用于减少生物负载生产纯化的重组蛋白质的连续方法。